Echinodorus cordifolius (L.)은 택사과(Alismataceae)에 속하는 수생 식물이다. Echinodorus cordifolius (L.)의 피부 항노화 활성에 대한 연구는 아직까지 보고되지 않았다. 따라서 본 연구의 목적은 Echinodorus cordifolius (L.)로부터 70% 에탄올 추출물 (ECEE)을 제조하고 항산화 및 hyaluronidase 억제 활성을 통해 피부 항노화 기능성 소재로서의 가능성을 확인하였다. ECEE는 EC50과 IC50 값이 각각 31.4, 300, 450 ㎍/mL로 우수한 DPPH, hydrogen peroxide 소거 활성 및 hyaluronidase 억제 활성을 가지는 것으로 나타났다. ECEE는 불멸화된 인간 각질세포인 HaCaT 세포에서 1 mM H2O2로 유도된 산화적 스트레스에 대해 농도 의존적으로 세포 보호 효과 증가 및 세포 내 활성산소종을 생성을 억제하는 것으로 나타났다. 또한 ECEE는 hyaluronic acid (HA) 합성 효소인 hyaluronan synthase 2 발현 수준을 상향 조절하고 HA 분해 효소인 hyaluronidase 2(HYAL2)의 발현 수준을 하향 조절하여 HaCaT 세포에서 HA 생성량을 증가시켰다. 따라서 이러한 결과를 종합하면 ECEE는 항산화, hyaluronidase 억제 및 HA 생성 촉진의 우수한 잠재력을 가진다는 것을 확인하였고 이를 통해 피부 노화를 방지하기 위한 천연 기능성 화장품 소재로서의 충분한 활용 가치가 있다고 판단된다.
Echinodorus cordifolius (L.)은 택사과(Alismataceae)에 속하는 수생 식물이다. Echinodorus cordifolius (L.)의 피부 항노화 활성에 대한 연구는 아직까지 보고되지 않았다. 따라서 본 연구의 목적은 Echinodorus cordifolius (L.)로부터 70% 에탄올 추출물 (ECEE)을 제조하고 항산화 및 hyaluronidase 억제 활성을 통해 피부 항노화 기능성 소재로서의 가능성을 확인하였다. ECEE는 EC50과 IC50 값이 각각 31.4, 300, 450 ㎍/mL로 우수한 DPPH, hydrogen peroxide 소거 활성 및 hyaluronidase 억제 활성을 가지는 것으로 나타났다. ECEE는 불멸화된 인간 각질세포인 HaCaT 세포에서 1 mM H2O2로 유도된 산화적 스트레스에 대해 농도 의존적으로 세포 보호 효과 증가 및 세포 내 활성산소종을 생성을 억제하는 것으로 나타났다. 또한 ECEE는 hyaluronic acid (HA) 합성 효소인 hyaluronan synthase 2 발현 수준을 상향 조절하고 HA 분해 효소인 hyaluronidase 2(HYAL2)의 발현 수준을 하향 조절하여 HaCaT 세포에서 HA 생성량을 증가시켰다. 따라서 이러한 결과를 종합하면 ECEE는 항산화, hyaluronidase 억제 및 HA 생성 촉진의 우수한 잠재력을 가진다는 것을 확인하였고 이를 통해 피부 노화를 방지하기 위한 천연 기능성 화장품 소재로서의 충분한 활용 가치가 있다고 판단된다.
Echinodorus cordifolius (L.) is an aquatic plant in the family Alismataceae. The anti-skin aging activity of E. cordifolius (L.) has not been yet reported. Therefore, the objective of the present study was to prepare 70% ethanol extract (ECEE) from E. cordifolius (L.) and investigate their antioxida...
Echinodorus cordifolius (L.) is an aquatic plant in the family Alismataceae. The anti-skin aging activity of E. cordifolius (L.) has not been yet reported. Therefore, the objective of the present study was to prepare 70% ethanol extract (ECEE) from E. cordifolius (L.) and investigate their antioxidant and anti-hyaluronidase activities for confirm the potential of anti-skin aging. ECEE showed good activities of DPPH, hydrogen peroxide scavenging, and hyaluronidase inhibition, with EC50 and IC50 values of 31.4, 300, and 450 ㎍/mL, respectively. ECEE also significantly improved cell viability and inhibited intracellular reactive oxygen species dose-dependently against 1 mM hydrogen peroxide-induced oxidative stress in immortalized human keratinocytes (HaCaT cells). Furthermore, ECEE upregulated hyaluronic acid (HA)-synthesizing enzyme hyaluronan synthase 2 (HAS2) expression level, but downregulated expression level of HA-degrading enzyme hyaluronidase 2, resulting in increased HA production in HaCaT cells. Taken together, these results suggest that ECEE shows antioxidant and anti-hyaluronidase potential and could be a functional cosmetic ingredients for anti-skin aging.
Echinodorus cordifolius (L.) is an aquatic plant in the family Alismataceae. The anti-skin aging activity of E. cordifolius (L.) has not been yet reported. Therefore, the objective of the present study was to prepare 70% ethanol extract (ECEE) from E. cordifolius (L.) and investigate their antioxidant and anti-hyaluronidase activities for confirm the potential of anti-skin aging. ECEE showed good activities of DPPH, hydrogen peroxide scavenging, and hyaluronidase inhibition, with EC50 and IC50 values of 31.4, 300, and 450 ㎍/mL, respectively. ECEE also significantly improved cell viability and inhibited intracellular reactive oxygen species dose-dependently against 1 mM hydrogen peroxide-induced oxidative stress in immortalized human keratinocytes (HaCaT cells). Furthermore, ECEE upregulated hyaluronic acid (HA)-synthesizing enzyme hyaluronan synthase 2 (HAS2) expression level, but downregulated expression level of HA-degrading enzyme hyaluronidase 2, resulting in increased HA production in HaCaT cells. Taken together, these results suggest that ECEE shows antioxidant and anti-hyaluronidase potential and could be a functional cosmetic ingredients for anti-skin aging.
Mukherjee PK, Maity N, Nema NK, Sarkar BK (2011) Bioactive compounds from natural resources against skin aging. Phytomedicine 19(1): 64-73. doi: 10.1016/j.phymed.2011.10.003?
Lee SJ, Choi HR, Lee JC, Park HJ, Lee HK, Jeong JT, Lee TB (2014) The anti-aging effects of various berries in the human skin keratinocyte (HaCaT) cells. Korean J Food Sci Technol 46(2): 198-204. doi: 10.9721/KJFST.2014.46.2.198?
Miracle Uwa L (2017) The anti-aging efficacy of antioxidants. Curr Trends Biomedical Eng & Biosci. 7(4): 66-68. doi: 10.19080/CTBEB.2017.07.555716?
Rinnerthaler M, Bischof J, Streubel MK, Trost A, Richter K (2015) Oxidative stress in aging human skin. Biomolecules 5(2): 545-589. doi: 10.3390/biom5020545?
Nguyen JK, Masub N, Jagdeo J (2020) Bioactive ingredients in Korean cosmeceuticals: Trends and research evidence. J Cosmet Dermatol 19(7): 1555-1569. doi: 10.1111/jocd.13344?
Faria-Silva C, Ascenso A, Costa AM, Marto J, Carvalheiro M, Ribeiro HM, Simoes S (2020) Feeding the skin: A new trend in food and cosmetics convergence. Trends Food Sci Technol 95: 21-32. doi: 10.1016/j.tifs.2019.11.015?
Ahmed IA, Mikail MA, Zamakshshari N, Hussah AS (2020) Natural anti-aging skincare: role and potential. Biogerontology 21: 293-310. doi: 10.1007/s10522-020-09865-z?
Cho YJ, Kim SH, Choi YJ, Lee JB (2022) Antioxidant and Anti-inflammatory activity of Colpomenia sinuosa extract. Journal of Convergence for Information Technology 12(1): 135-143. doi: 10.22156/CS4SMB.2022 12.01.135?
Lee TB, So YK, Kim SY, Hwang JY (2020) Biological Activities of Cosmetic Material from Ten Kinds of Flower Ethanol Extracts. Korean J. Medicinal Crop Sci. 28(4): 260-275. doi: 10.7783/KJMCS.2020.28.4.260?
Teamkao P, Thiravetyan P (2010) Phytoremediation of ethylene glycol and its derivatives by the burhead plant (Echinodorus cordifolius (L.)): Effect of molecular size. Chemosphere 81: 1069-1074. doi: 10.1016/j.chemosphere.2010.09.049?
Bercu R (2009) Histoanatomy of Echinodorus cordifolius (L.) Griseb. (Alismataceae). Studia Univ. VG, SSV 19: 283-286?
Yu SR, Kim SH, Shin SM (2018) The effect of Alisma canaliculatum and Polyporus umbellatus extracts on adipogenic differentiation of human bone marrow derived mesenchymal stromal stem cells. Korean J Orient Int Med 39: 1181-1190. doi: 10.22246/jikm.2018.39.6.1181?
Muller HE (1995) Detection of hydrogen peroxide produced by microorganism on ABTS peroxidase medium. Zentralbl Bakterio Mikrobio Hyg 259: 151-158. doi: 10.1016/S0176-6724(85)80045-6?
AOAC (1980) Official methods of analysis. 13th ed, Association of Official Analysis Chemists, Washington, DC, USA. Method 914-915?
Im ST, Kang NL, Kim JS, Heo SJ, Lee SH (2022) Antioxidant and anti-hyaluronidase activities of taurine-enriched enzymatic hydrolysates prepared from Turbo cornutus, a by-product of fish processing in Jeju Island. Fish Sci 88: 509-517. doi: 10.1007/s12562-022-01605-1?
Yoon Y, Lee YM, Song S, Lee YY, Yeum KJ (2018) Black soybeans protect human keratinocytes from oxidative stress-induced cell death. Food Sci. Nutr. 6: 2423-2430. doi: 10.1002/fsn3.842?
Herrmann K (1989) Occurrence and content of hydroxycinnamic and hydroxybenzoic acid compounds in foods. Crit Rev Food Sci Nutr 28: 315-347. doi: 10.1080/10408398909527504?
Kim PJ, Ha HY (2018) Effect of Lonicera japonica ethanol extract on hyaluronic acid production in HaCaT cells. J Invest Cosmetol 14: 441-446. doi: 10.15810/jic.2018.14.4.005?
Razak DLA, Jamaluddin A, Rashid NYA, Sani NA, Manan MA (2020) Assessment of Cosmeceutical Potentials of Selected Mushroom Fruitbody Extracts Through Evaluation of Antioxidant, Anti-Hyaluronidase and Anti-Tyrosinase Activity. J 3(3): 329-342. doi: 10.3390/j3030026?
Seo SH, Choi MO (2013) Protectvie effects of Lonicerae Japonicae Flos against hydrogen peroxidase-induced oxidative stress on Human keratinocyte, HaCaT cells. Kor J Herbology 28: 57-62. doi: 10.6116/kjh.2013.28.4.57?
Chang TM, Tsen JH, Yen H, Yang TY, Huang HC (2017) Extract from Periostracum cicadae Inhibits Oxidative Stress and Inflammation Induced by Ultraviolet B Irradiation on HaCaT Keratinocytes. Evid Based Complement Alternat Med 2017. doi: 10.1155/2017/8325049?
Kim AR, Jung MC, Jeong HI, Song DG, Seo YB, Jeon YH, Park SH, Shin HS, Lee SL, Park SN (2018) Antioxidative and cellular protective effects of Lysimachia christinae hance extract and fractions. Appl Chem Eng 29: 176-184. doi: 10.14478/ace.2017.1113?
Kolakul P, Sripanidkulchai B (2017) Phytochemicals and anti-aging potentials of the extracts from Lagerstroemia speciosa and Lagerstroemia floribunda. Ind Crops Prod 109: 707-716. doi: 10.1016/ j.indcrop.2017.09.026?
Kang MS, Ha HY, Kim HT (2015) An experimental study on the effect of Angelica acutiloba ethanol extract on hyaluronic acid synthesis. J Korean Med Ophthalmol Otolaryngol Dermatol 28: 32-40. doi: 10.6114/jkood.2015.28.1.032
※ AI-Helper는 부적절한 답변을 할 수 있습니다.