[논문]Neuroprotective effect of Coreopsis lanceolata extract against hydrogen-peroxide-induced oxidative stress in PC12 cells

Kyung Hye Seo; Hyung Don Kim; Jeong-Yong Park; Dong Hwi Kim; Seung-Eun Lee; Gwi Young Jang; Yun-Jeong Ji; Ji Yeon Lee

doi:10.7744/kjoas.20220014

Neuroprotective effect of Coreopsis lanceolata extract against hydrogen-peroxide-induced oxidative stress in PC12 cells 원문보기

Korean journal of agricultural science, v.49 no.2, 2022년, pp.175 - 184

Kyung Hye Seo (Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration) , Hyung Don Kim (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) , Jeong-Yong Park (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) , Dong Hwi Kim (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) , Seung-Eun Lee (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) , Gwi Young Jang (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) , Yun-Jeong Ji (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration) , Ji Yeon Lee (Development of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Rural Development Administration)

Abstract ▼ AI-Helper

The present study investigated the neuroprotective effects of Coreopsis lanceolate extract against hydrogen-peroxide (H2O2)-induced oxidative damage and cell death in pheochromocytoma 12 (PC12) cells. Reactive oxygen species (ROS), 2,2'-azinobis (3-ethylbebzothiazoloine-6-sulfonic acid) diammonium salt, and 1,1-diphenyl-2-picrrylhydrazyl radical scavenging activities, as well as the expression levels of proteins associated with oxidative damage and cell death were investigated. According to the results, C. lanceolate extract exhibited inhibitory activity against intracellular ROS generation and cell-damaging effects induced by hydroxyl radicals in a dose-dependent manner. Total phenolic and flavonoid contents were 22.3 mg·g-1 gallic acid equivalent and 16.2 mg·g-1 catechin equivalent, respectively. Additionally, a high-performance liquid chromatography (HPLC) assay based on the internal standard method used to detect phenolic compounds. The phenolic compounds identified in C. lanceolata extract contained (+)-catechin hydrate (5.0 ± 0.0 mg·g-1), ferulic acid (1.6 ± 0.0 mg·g-1), chlorogenic acid (1.5 ± 0.0 mg·g-1), caffeic acid (1.2 ± 0.0 mg·g-1), naringin (0.9 ± 0.0 mg·g-1), and p-coumaric acid (0.5 ± 0.0 mg·g-1). C. lanceolata extract attenuated pro-apoptotic Bax expression levels and enhanced the expression levels of anti-apoptotic Bcl-2, caspase-3, and caspase-9 proteins. Therefore, C. lanceolata is a potential source of materials with neuroprotective properties against neurodegenerative disorders, such as Alzheimer's and Parkinson's diseases.

주제어

표/그림 (6)

표 Table 1. Antioxidant components and activities of CLA.
그림 Fig. 1. The chromatographys of phenolics (100 μg·mL^-1; A) and CLA (10 mg·mL^-1; B) using by high-performance liquid chromatography (HPLC). Peak identification: 1, gallic acid; 2, homogentisic acid; 3, chlorogenic acid; 4, (+) -catechin hydrate; 5, caffeic acid; 6, p- coumaric acid; 7, ferulic acid; 8, naringin; 9, quercetin hydrate; 10, cinnamic acid. CLA, aerial parts of Coreopsis lanceolate.
표 Table 2. Phenolics contents of CLA analyzed by high-performance liquid chromatography (HPLC) (n = 3).
그림 Fig. 2. Protect effect of intracellular reactive oxygen species (ROS) generation of CLA on H₂O₂-induced PC12cells. The data indicated that CLA against oxidative stress induced PC12 cells in a dose-dependent manner. All values are means ± SD, n = 3. ^##p < 0.01 comparing H₂O₂ non- treated control and H₂O₂ treated control column; ^**p < 0.01 and ^***p < 0.001, comparing H₂O₂ treated control and each column. CLA, aerial parts of Coreopsis lanceolate.
그림 Fig. 3. Cell viability of CLA extracts (50, 100, 200 μg·mL^-1) on H₂O₂-induced PC12cells by MTS assay. All values are means ± SD (n = 3). Values with different superscript letters indicate a statistically significant difference determined by Duncan's test (p < 0.05). CLA, aerial parts of Coreopsis lanceolate; MTS, [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium].
그림 Fig. 4. Protective effect of apoptotic factor of CLA H₂O₂ induced PC12cells. (A) Protein expression related with intrinsic pathway (Bax, Bcl-xL and cytochrome C). (B) Protein expression related with extrinsic pathway (caspase-3 and caspase-9). Cells were treated with CLA (50, 100, and 200 μg·mL^-1) for 24h and then treated with H₂O₂ (50 μM) for 20 min. CLA, aerial parts of Coreopsis lanceolate.

참고문헌 (30)

Balasundram N, Sundram K, Samman S. 2006. Phenolic compounds in plants and agri-industrial by-products:？Antioxidant activity, occurrence, and potential uses. Food Chemistry 99:191-203.

상세보기
Bidchol AM, Wilfred A, Abhijn P, Abhijn R. 2011. Free radical scavenging activity of aqueous and ethanolic extract of？Brassica oleracea L. var. italica. Food and Bioprocess Technology 4:1137-1143.

상세보기
Boatright KM, Salvesen GS. 2003. Mechanisms of caspase activation. Current Opinion in Cell Biology 15:725-731.

상세보기
Chung IM, Park MR, CHun JC, Yun SJ. 2003. Resveratrol accumulation and resveratrol synthase gene expression in？response to abiotic stresses and hormones in peanut plants. Plant Science 164:103-109.

상세보기
Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. 2008. Iron chelating activity, phenol and flavonoid content of some？medicinal plants from Iran. African Journal of Biotechnology 7:3188-3192.
Folin O, Denis W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. Journal of Biological？Chemistry 12:239-243.

상세보기
Heo HJ, Kim DO, Choi SJ, Shin DH, Lee CY. 2004. Potent inhibitory effect of flavonoids in Scutellaria baicalensis on？amyloid β protein-induced neurotoxicity. Journal of Agricultural and Food Chemistry 52:4128-4132.

상세보기
Ho CT. 1992. Phenolic compounds in food: An overview. ACS Publications 507:2-7.
Ilavenil S, Arasu MV, Lee JC, Kim DH, Roh SG, Park HS, Choi GJ, Mayakrishnan V, Choi KC. 2014. Trigonelline attenuates？the adipocyte differentiation and lipid accumulation in 3T3-L1 cells. Phytomedicine 21:758-765.

상세보기
Kim HG, Oh HJ, Ko JH, Song HS, Lee YG, Kang SC, Lee DY, Baek NI. 2019. Lanceoleins A-G, hydroxychalcones, from the？flowers of Coreopsis lanceolata and their chemopreventive effects against human colon cancer cells. Bioorganic？Chemistry 85:274-281.
Kim MY, Jang GY, Lee SH, Kim KM, Lee JS, Jeong HS. 2018. Preparation of black soybean (Glycine max L) extract？with enhanced levels of phenolic compound and estrogenic activity using high hydrostatic pressure and pregermination. High Pressure Research 38:177-192.

상세보기
Ko MS, Lee HJ, Kang MJ. 2012. Antioxidant activities and whitening effects of extracts from Hippophae rhamnoides L.？Journal of the East Asian Society of Dietary Life 22:812-817.
Lee AY, Kim MJ, Lee SH, Shim JS, Cho EJ. 2018. Protective effect of Cirsium japonicum var. maackii against oxidative？stress in C6 glial cells. Korean Journal of Agricultural Science 45:509-519.[in Korean]

원문보기 상세보기
Li P, Nijhawan D, Budihardjo I, Srinivasula SM, Ahmad M, Alnemri ES, Wang X. 1997. Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade. Cell 91:479-489.

상세보기
Mandel S, Youdim MB. 2004. Catechin polyphenols: Neurodegeneration and neuroprotection in neurodegenerative？diseases. Free Radical Biology and Medicine 37:304-317.

상세보기
Masella R, Benedetto RD, Vari R, Filesi C, Giovannini C. 2005. Novel mechanisms of natural antioxidant compounds？in biological systems: Involvement of glutathione and glutathione-related enzymes. The Journal of Nutritional？Biochemistry 16:577-586.

상세보기
Meister A. 1992. On the antioxidant effects of ascorbic acid and glutathione. Biochemical Pharmacology 44:1905-1915.

상세보기
Nam MN, Lee AY, Sin SM, Goo YM, Cho EJ. 2019. Protective effect of Samultang and its four herbal plants against？reactive oxygen speciesin vitro and cellular system. Korean Journal of Agricultural Science 46:427-437.

원문보기 상세보기
Ozben T. 2007. Oxidative stress and apoptosis: Impact on cancer therapy. Journal of Pharmaceutical Sciences？96:2181-2196.
Park SJ. 2014. Antioxidant and anti-adipogenic effects of ethanolic extracts from Ixeris dentata Nakai. Culinary Science？and Hospitality Research 20:133-142.
Pourmorad F, Hosseinimehr S, Shahabimajd N. 2006. Antioxidant activity, phenol and flavonoid contents of some？selected Iranian medicinal plants. African Journal of Biotechnology 5:1142-1145.
Rains TM, Agarwal S, Maki KC. 2011. Antiobesity effects of green tea catechins: A mechanistic review. The Journal of？Nutritional Biochemistry 22:1-7.

상세보기
Reichling J, Horz KH, Ohlenmacher U. 1994. Coreopsis spp: In vitro culture and the formation of phenylpropanoids？and other secondary metabolites. In Medicinal and Aromatic Plants VI. pp. 106-128. Springer, Berlin, Heidelberg.
Reichling J, Thron U. 1989. Comparative study on the production and accumulation of unusual phenylpropanoids in？plants and in vitro cultures of Coreopsis tinctoria and C. lanceolata. Pharmaceutisch Weekblad 11:83-86.
Rice-Evans C, Miller N. 1996. Antioxidant activities of flavonoids as bioactive components of food. Biochemical Society？Transactions 24:790-795.

상세보기
Simon HU, Haj-Yehia A, Levi-Schaffer F. 2000. Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis？5:415-418.

상세보기
Srinivasula SM, Hegde R, Saleh A, Datta P, Shiozaki E, Chai J, Lee RA, Robbins PD, Fernandes-Alnemri T, Shi Y, et？al. 2001. A conserved XIAP-interaction motif in caspase-9 and Smac/DIABLO regulates caspase activity and？apoptosis. Nature 410:112-116.

상세보기
Tait SW, Green DR. 2010. Mitochondria and cell death: Outer membrane permeabilization and beyond. Nature？Reviews Molecular Cell Biology 11:621-632.

상세보기
Yang E, Zha J, Jockel J, Boise LH, Thompson CB, Korsmeyer DJ. 1995. Bad, a heterodimeric partnerfor Bcl-XL and Bcl-2, displaces Bax and promotes cell death. Cell 80:285-291.

상세보기
Zhang Y, Zhang Y, Shi S, Zhao M, Jiang Y, Tu P. 2006. A novel chalcone from Coreopsis tinctoria Nutt. Biochemical？Systematics and Ecology 10:766-769.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증