[논문]모유 유래 유산균 Enterococcus faecalis BMSE-HMP005의 다제내성 균에 대한 항균효과

이정은; 김수빈; 유두나; 조소연; 김애정; 국무창

doi:10.9721/kjfst.2022.54.2.209

모유 유래 유산균 Enterococcus faecalis BMSE-HMP005의 다제내성 균에 대한 항균효과
Antimicrobial effect of Enterococcus faecalis BMSE-HMP005 isolated from human breast milk against multidrug-resistant bacteria 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.54 no.2, 2022년, pp.209 - 217

이정은 (경기대학교 일반대학원 대체의학과) , 김수빈 (중앙대학교 식품영양학과) , 유두나 (경희대학교 생명공학원) , 조소연 (배화여자대학교 식품영양학과) , 김애정 (경기대학교 대체의학대학원) , 국무창 (배화여자대학교 식품영양학과)

초록
AI-Helper

본 연구에서는 모유 유래 유산균 E. faecalis BMSE-HMP005의 다제내성 균주에 대한 항균효과 및 probiotics로써 잠재적인 가능성을 확인하였다. E. faecalis BMSE-HMP005는 다제내성 균주 20주(Enterococcus spp., Staphylococcus spp., Escherichia spp., Pseudomonas spp., Salmonella spp., Klebsiella spp., Enterobacter spp.)에서 모두 MBC가 확인되어 다제내성 균주에 대한 우수한 항균효과를 입증하였다. 또한 RP-HPLC를 이용하여 배양액 내 bacteriocin을 확인하였으며, 이에 대한 분획은 gram 양성 및 gram 음성균주에서 모두 항균력이 나타나, E. faecalis BMSE-HMP005가 생산하는 bacteriocin의 광범위한 항균 스펙트럼을 입증하였다. E. faecalis BMSE-HMP005는 발암 화합물을 유발하는 β-glucuronidase에 대한 활성과 용혈성은 나타나지 않아 안전한 것으로 판단된다. E. faecalis BMSE-HMP005는 vancomycin에 대해서는 내성을 보이나, kanamycin (>0.058), ampicillin (>0.002), erythromycin (>0.002)은 EFSA 기준의 허용범위보다 낮은 MIC가 확인되었다. 또한 인공위액(pH 2.0) 및 인공 담즙산(0.3% bile acid) 조건에서 각각 98.67%, 95.70%의 생존율을 보였다. 이와 같은 결과는 본 연구에서 분리한 모유 유래 유산균 E. faecalis BMSE-HMP005가 다제내성 균주에 대한 우수한 항균활성을 갖는 probiotics로써 잠재적인 가능성을 보여준다. 따라서 건강기능식품의 기준 및 규격에 제시된 바와 같이 Enterococcus spp.는 항생제 내성 및 독성 유전자가 없는 경우에 한하여 probiotics로 사용이 가능한 고시하고 있어 E. faecalis BMSE-HMP005의 안전성에 대한 추가적인 검증이 필요할 것으로 판단된다.

Abstract ▼ AI-Helper

In this study, Enterococcus faecalis BMSE-HMP005 isolated from human breast milk demonstrated antimicrobial effects against multidrug-resistant (MDR) bacterial strains. The bacteriocin produced by E. faecalis BMSE-HMP005 was fractionated using reverse-phase high-performance liquid chromatography. This fraction showed antimicrobial effects against both gram-positive and gram-negative MDR bacteria. No hemolytic reactions were observed. E. faecalis BMSEHMP005 was resistant to vancomycin; however, kanamycin, ampicillin, and erythromycin showed minimum inhibitory concentrations that were lower than the acceptable range provided by the European Food Safety Authority. For artificial gastric juice and bile acid, the survival rates were 98.67% and 95.70%, respectively. These results show the potential utility of E. faecalis BMSE-HMP005 as a probiotic with remarkable antimicrobial effects against MDR bacteria.

주제어

표/그림 (8)

표 Table 1. List of indicator strains
표 Table 2. Enzyme assay for biochemical reactions of Enterococcus faecalis BMSE-HMP005 using API ZYM kit
표 Table 3. Determination of the MIC and MBC of Enterococcus faecalis BMSE-HMP005 against multidrug-resistant bacteria
그림 Fig. 1. Release of DNA. (A); Enterococcus faecalis CCARM 5171, (B); Staphylococcus aureus CCARM 3855, (C); Escherichia coli DC 2 CCARM 0238, (D); Pseudomonas aeruginosa CCARM 0223, (E); Salmonella enterica CCARM 0240, (F); Klebsiella oxytoca CCARM 0248, (G); Enterobacter cloacae P 99 CCARM 0252. Bar graph showed mean±SD of released DNA concentration. p values refer to the analysis by unpaired t-test. ***p<0.001: MIC vs MBC, control vs MIC by unpaired t-test.
그림 Fig. 2. RP-HPLC chromatogram of a cultured supernatant from Enterococcus faecalis BMSE-HMP005 using ACE C18 column. The retention time of a bacteriocin as metabolite was 22.00 min. (A); MRS broth, (B); supernatant. Indicators for the antimicrobial assay were (a); S. aureus KCCM 11335, (b); E. coli KCTC 2571, (c); S. aureus CCARM 3855, (d); E. coli DC 2 CCARM 0238, (e); P. aeruginosa CCARM 0223.
그림 Fig. 3. Hemolysis of Enterococcus faecalis BMSE-HMP005 isolated from human breast milk.
표 Table 4. Antibiotic sensitivity and resistance of Enterococcus faecalis BMSE-HMP005
그림 Fig. 4. Survival effects of pH 2.0 and 0.3% bile acid of Enterococcus faecalis BMSE-HMP005 isolated from human breast milk. Data are presented as mean±SD of three independent experiments. □; 0 time, ■; after 3 hours.

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