[논문]우리나라 콩 생육초기 바이러스병 발생 양상

박상민; 권민아; 강동현; 이홍규; 윤영남; 백인열; 이영규; 문제선; 이수헌

doi:10.7740/kjcs.2022.67.4.253

우리나라 콩 생육초기 바이러스병 발생 양상
Occurrence of Viral Diseases in the Early Growth Stage of Soybean in Korea 원문보기

Korean journal of crop science = 韓國作物學會誌, v.67 no.4, 2022년, pp.253 - 264

박상민 (경북대학교 응용생명과학부) , 권민아 (경북대학교 응용생명과학부) , 강동현 (경북대학교 응용생명과학부) , 이홍규 (경북대학교 응용생명과학부) , 윤영남 (국립식량과학원 남부작물부 생산기술개발과) , 백인열 (국립식량과학원 남부작물부 밭작물개발과) , 이영규 (국립식량과학원 고령지농업연구소) , 문제선 (한국생명공학연구원 식물시스템공학연구센터) , 이수헌 (경북대학교 응용생명과학부)

초록
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본 연구는 콩 바이러스병 관리대책 수립을 위하여 생육 초기에 발생하는 바이러스병의 감염실태를 조사하였다. 국립식량과학원 육종포장에서 파종 후 약 3-4주 된 콩 이상증상 시료 83점을 채집하였다. 채집한 시료의 감염 바이러스 진단은 12종 바이러스의 RT-PCR 진단과 전체 시료에 대한 메타전사체 분석을 통하여 수행하였다. 1. 포장에서 바이러스병 발병율은 전체적으로 1% 미만으로 조사되었으며 일부 품종 및 라인에서는 최대 50%까지 나타내었다. 2. RT-PCR 진단과 메타전사체 분석 결과 SYMMV, SMV, SYCMV, PSV, SGVA가 검출되었다. 3. 검출된 바이러스 중에서 SYMMV와 SMV는 각각 검출율이 53.7%, 42.6%로 나타내어 콩 생육초기에 발생하는 주요 바이러스로 확인되었다. 4. SYMMV에 감염된 콩은 전형적인 모자이크 증상을 나타내었으며, SMV에 감염된 콩은 모자이크, 얼룩, 위축, 퇴록반점과 같이 다양한 증상을 나타내었다. 5. 바이러스 전염 특성을 고려할 때 SMV는 주로 종자전염을 통하여 발생한 것으로 판단되며, SYMMV는 종자전염과 함께 충매전염 및 토양전염의 가능성이 복합적으로 고려되어야 할 것으로 판단된다. 6. SGVA는 본 연구를 통하여 콩 생육초기 포장에서 검출되었으며, 생육초기 이후 발생실태 및 콩에 미치는 영향에 관한 연구가 신속히 수행되어야 할 것이다.

Abstract ▼ AI-Helper

In this study, we investigated the occurrence of viral diseases in the early growth stage of soybean to establish management practices. We collected 83 soybean samples showing abnormal symptoms, approximately 3-4 weeks after seeding in the breeding field of the National Institute of Crop Science. Viruses were detected in the collected samples using reverse transcription polymerase chain reaction (RT-PCR) and metatranscriptome analysis of all those samples. The incidence of viral diseases in the field was less than 1% overall and up to 50% in certain cultivars and lines. RT-PCR and metatranscriptome analysis detected Soybean yellow mottle mosaic virus (SYMMV), Soybean mosaic virus (SMV), Soybean yellow common mosaic virus, Peanut stunt virus, and soybean geminivirus A (SGVA). Among these detected viruses, SYMMV and SMV were identified as major viruses causing infection in the early growth stage of soybean, with detection rates of 53.7% and 42.6%, respectively. Soybeans infected with SYMMV showed typical mosaic symptoms, whereas those infected with SMV showed a variety of symptoms such as mosaic, mottle, stunt, and chlorotic spots. Transmission characteristics of these viruses are variable, such that SMV is primarily transmitted by seeds, whereas SYMMV could be transmitted by insects, soil, and seeds. In this study, SGVA was detected in the early growth stage of soybean, and research on the current status and its effects on soybean after the early growth stage should be conducted.

주제어

표/그림 (7)

표 Table 1. List of viruses found in soybean in Korea.
표 Table 2. Primer sets for multiplex RT-PCR of 11 soybean virus species.^a
표 Table 3. Investigation of the incidence of viral diseases in the early growth stage using the cultivars, lines, symptoms, and detected viruses of soybean.^a,b
그림 Fig. 1. Genomic structure of the plant viruses and location of plant virus-associated contigs detected through metatranscriptome analysis. The blue bars indicate detected contigs and are located in relative positions to the reference genome. The reference genome used the sequence with the highest similarity in BLASTn analysis. A: Soybean yellow mottle mosaic virus (GenBank : MT603848), B: Soybean mosaic virus (KY986929), C: Soybean yellow common mosaic virus (LC332541), D: Soybean geminivirus A (MH428829), E: Peanut stunt virus (LC380689).
그림 Fig. 2. The proportion of viruses detected in soybean in the early growth stage. Virus detection was confirmed via RT-PCR and metatranscriptome analysis. (A) the sample ratio of detected and undetected viruses; and (B) individual virus proportion in the infected samples. Coinfection of Soybean yellow mottle mosaic virus and Soybean mosaic virus was confirmed in one sample.
그림 Fig. 3. Symptoms in virus-infected soybeans in the early growth stage. Soybeans were collected approximately 3-4 weeks after seeding. Infection with the following five virus species was confirmed via RT-PCR: Soybean yellow mottle mosaic virus (SYMMV), Soybean mosaic virus (SMV), Soybean yellow common mosaic virus (SYCMV), Peanut stunt virus (PSV), and soybean geminivirus A (SGVA). Soybeans infected with SYMMV (A-C) showed distinct mosaic symptoms, whereas those infected with SMV (D-K) showed various symptoms such as mosaic, mottle, stunt, and chlorotic spots. Samples coinfected with SYMMV and SMV (L) were similar to those infected with SYMMV. Soybeans infected with SYCMV (M) exhibited chlorosis and golden mosaic symptoms. PSV (N) infection caused severe symptoms in soybeans, such as mosaic, vein banding, and necrotic spots, whereas soybeans infected with SGVA (O) showed chlorotic spots.
그림 Fig. 4. Various abnormal symptoms observed in the early growth stages of soybean. No virus was detected in the samples via RT-PCR and metatranscriptome analysis; therefore, abnormal symptoms were presumably caused by physiological disorders and insects. (A and B) chlorotic spots; (C) yellowing, distortion, and malformation; (D-F) yellowing; (G-I) distortion and malformation; (J and K) vein banding and vein necrosis; and (L) chlorosis and yellow spots.

참고문헌 (34)

Cho, E. K. 1981. Strains of soybean mosaic virus with emphasis？on the mechanism of aphid transmissibility. Korean J. Pl. Prot.？20(3) : 177-180.
Cho, E. K. 1995. Identification of Soybean Mosaic Virus Strains？and a Consideration on Genetics of Soybean for Resistance to？SMV Strains. Plant Dis. and Agriculture. 1(2) : 22-25.
Cho, E. K. and B. J. Chung. 1976. Studies on Identification and？Classification of Soybean Virus Diseases in Korea. Korean J.？Plant Prot. 15(2) : 61-68.
Cho, E. K. and R. M. Goodman. 1982. Field Spread of Soybean？Mosaic Virus Strains. Korean J. Plant Prot. 21(2) : 53-60.
Cho, E. K. and S. H. Lee. 2003. Cowpea mosaic virus from？Vegetable Soybeans in Korea. Plant Pathol. J. 19(3) : 166-170.

원문보기 상세보기
Cho, E. K., S. H. Choi, and C. Y. Hwang. 1984. Epidemiology of？Soybean Mosaic Virus Diseases. Korean J. Plant Prot. 23(4) :？197-202.
Cho, S., J. Kim, M. Li, E. Seo, S. Lim, S. M. Hong, J. S. Moon, J.？Hammond, and H. S. Lim. 2013. Occurrence of Three Major？Soybean Viruses, Soybean mosaic virus, Soybean yellow mottle？mosaic virus and Soybean yellow common mosaic virus？Revealed by a Nationwide Survey of Subsistence Farming？Soybean Fields. Res. Plant Dis. 19(4) : 319-325.
Fiallo-Olive, E., J. M. Lett, D. P. Martin, P. Roumagnac, A.？Varsani, F. M. Zerbini, and J. Navas-Castillo. 2021. ICTV？Virus Taxonomy Profile: Geminiviridae 2021. J. Gen. Virol.？102(12) : 1-2.
Kil, E. J., J. Park, H. S. Choi, C. S. Kim, and S. Lee. 2017. Seed？Transmission of Tomato yellow leaf curl virus in White？Soybean (Glycine max). Plant Pathol. J. 33(4) : 424-428.
Kim, J. S., S. H. Lee, H. S. Choi, M. K. Kim, H. R. Kwak, J. S.？Kim, M. Nam, J. D. Cho, I. S. Cho, and G. S. Choi. 2012.？2007-2011 Characteristics of Plant Virus Infections on Crop？Samples Submitted from Agricultural Places. Res. Plant Dis.？18(4) : 277-289.
Kim, S. M., J. B. Kim, Y. H. Lee, S. H. Choi, H. S. Choi, J. W.？Park, J. S. Lee, G. S. Lee, J. K. Moon, J. S. Moon, K. W. Lee,？and S. H. Lee. 2006. First Report of Soybean Dwarf Virus on？Soybean (Glycine max) in Korea. Res. Plant Dis. 12(3) :？213-220.
Kim, Y. H., O. S. Kim, B. C. Lee, J. K. Moon, S. C. Lee, and J. Y.？Lee. 2003. G7H, a new Soybean mosaic virus strain: Its？virulence and nucleotide sequence of CI gene. Plant Dis. 87 :？1372-1375.

상세보기
Lee, H., W. Song, H. R. Kwak, J. D. Kim, J. Park, C. K. Auh, D.？H. Kim, K. Y. Lee, S. Lee, and H. S. Choi. 2010. Phylogenetic？analysis and inflow route of Tomato yellow leaf curl virus？(TYLCV) and Bemisia tabaci in Korea. Mol. Cells. 30 :？467-476.

원문보기 상세보기
Lee, J. D., J. K. Yu, Y. H. Hwang, S. Blake, Y. S. So, G. J. Lee, H.？T. Nguyen and J. Grover Shannon. 2008. Genetic Diversity of？Wild Soybean (Glycine soja Sieb. and Zucc.) Accessions from？South Korea and Other Countries. Crop Science 48 : 606-616.

상세보기
Lee, S. H., Y. M. Choi, J. S. Kim, and B. J. Chung. 1985.？Identification of Alfalfa Mosaic Virus from Soybean. Korean？J. Plant Pathol. 1(1) : 33-37.
Lee, S. H., M. H. Lee, and H. Tochihara. 1980. An infectious？virus isolated from soybeans. Korean J. Plant Prot. 9(3) :？175-179.
Lee, S. H. and C. S. Kim. 2013. Natural Hosts and Disease Cycle？of Soybean yellow mottle mosaic virus. Res. Plant Dis. 19(4) :？281-287.
Lee, S. H., J. B. Lee, S. M. Kim, H. S. Choi, J. W. Park, J. S. Lee,？K. W. Lee, and J. S. Moon. 2004. The Incidence and Distribution of Viral Diseases in Pepper by Cultivation Types.？Res. Plant Dis. 10(4) : 231-240.
Lee, S. H., M. Nam, H. S. Choi, J. S. Lee, J. S. Kim, I. Y. Baek,？and J. K. Moon. 2011. Specific primers for multiple detection？of the viruses in soybean, and uses thereof. R.O.K Patent？10-1250388.
Lee, T. S. 2009. The Life Cycle of SYMMV Infected in Soybean.？Master's thesis, Jeonbuk National University.
Lee, Y. H. and T. S. Park. 2006. Origin of Legumes Cultivation in？Korea Peninsula by Viewpoint of Excavated Grain Remains？and Genetic Diversity of Legumes. Korean Agricultural？History Association 5(1) : 1-31.
Lee, Y. H., S. T. Lim, Y. N. Yoon, M. G. Jeon, H. T. Yun, J. M.？Ko, S. H. Lee, K. W. Lee, and I. Y. Baek. 2012. Incidence of？Soybean Viral Diseases in Korea. Korea Soybean Digest.？29(1) : 7-15.
Lee, Y. H., Y. N. Yoon, H. T. Yun, I. Y. Baek, S. Lim, J. S. Moon,？and S. H. Lee. 2015. First Report of Bean common mosaic？virus Infecting Soybean in South Korea. Plant Dis. 99(8) : 1189.

상세보기
Lim, S., Y. H. Lee, D. Igori, F. Zhao, R. H. Yoo, S. H. Lee, I.Y.？Baek, and J. S. Moon. 2014. First Report of Peanut mottle？virus Infecting Soybean in South Korea. Plant Dis. 98(9) : 1285.

상세보기
Lim, W. S., Y. H. Kim, and K. H. Kim. 2003. Complete Genome？Sequences of the Genomic RNA of Soybean mosaic virus？Strains G7H and G5. Plant Pathol. J. 19(3) : 171-176.
MAFRARK. 2021. Statistical Yearbook of Agriculture, Food and？Rural Affairs, Sejong, Republic of Korea.
Nam, M., J. S. Kim, S. J. Park, C. Y. Park, J. S. Lee, H. S. Choi, J.？S. Kim, H. H. Kim, S. Lim, J. S. Moon, and S. H. Lee. 2012a.？Biological and molecular characterization of Soybean yellow？common mosaic virus, a new species in the genus Sobemovirus.？Virus Res. 163(1) : 363-367.

상세보기
Nam, M., S. M. Kim, L. L. Domier, S. Koh, J. K. Moon, H. S.？Choi, H. G. Kim, J. S. Moon, and S. H. Lee. 2009. Nucleotide？sequence and genomic organization of a newly identified？member of the genus Carmovirus, soybean yellow mottle？mosaic virus, from soybean. Arch Virol. 154(10) : 1679-1684.

상세보기
Nam, M., S. J. Park, Y. J. Kim, J. S. Kim, C. Y. Park, J. S. Lee, H.？S. Choi, J. S. Kim, and S. H. Lee. 2012b. First Report of？Peanut stunt virus on Glycine max in Korea. Plant Pathol. J.？28(3) : 330.
Park, C. Y., H. G. Min, H. K. Lee, R. Maharjan, Y. Yoon, and S.？H. Lee. 2018. Development of a Multiplex Reverse Transcription-Polymerase Chain Reaction Assay for the Simultaneous Detection of Three Viruses in Leguminous Plants.？Res. Plant Dis. 24(4) : 348-352.
Sandra, N., A. Tripathi, H. K. Dikshit, B. Mandal, and R. K. Jain. 2020. Seed transmission of a distinct soybean yellow mottle？mosaic virus strain identified from India in natural and？experimental hosts. Virus Res. 280 : 197903.

상세보기
Seo, J. K., J. Y. Park, H. R. Kwak, M. K. Kim, M. Nam, S. H. Lee,？J. S. Kim, and H. S. Choi. 2014. Severe Outbreak of the？Emerging Soybean Viruses in Korea: An Increasing Threat.？Plant Health Prog. 15(3) : 122.
Shin, J. C., M. K. Kim, H. R. Kwak, H. S. Choi, J. S. Kim, C. Y.？Park, and B. J. Cha. 2014. First Report of Clover yellow vein？virus on Glycine max in Korea. Plant Dis. 98(9) : 1283.

상세보기
Yoon, Y. N., Y. Jo, W. K. Cho, H. Choi, Y. Jang, Y. H. Lee, J. Y.？Bae, and B. C. Lee. 2017. First Report of Tomato spotted wilt？virus Infecting Soybean in Korea. Plant Dis. 102(2) : 461.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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