[논문]산백국(山白菊) 열수추출물이 HaCaT 피부각질세포에서 미치는 항염증 효과

김세중; 이규영; 김근립; 홍철희

doi:10.6114/jkood.2022.35.2.001

산백국(山白菊) 열수추출물이 HaCaT 피부각질세포에서 미치는 항염증 효과
Effects of Aster glehni Water Extracts on Skin Inflammation in HaCaT Keratinocytes 원문보기

韓方眼耳鼻咽喉皮膚科學會誌 = The journal of Korean Medicine Ophthalmology & Otolaryngology & Dermatology, v.35 no.2, 2022년, pp.1 - 12

김세중 (상지대학교 한의과대학 안이비인후피부과학교실) , 이규영 (상지대학교 한의과대학 안이비인후피부과학교실) , 김근립 (상지대학교 한의과대학 안이비인후피부과학교실) , 홍철희 (상지대학교 한의과대학 안이비인후피부과학교실)

Abstract ▼ AI-Helper

Objectives : This study was conducted to verify the anti-inflammatory effects of Aster glehni water extracts in HaCaT keratinocytes. Methods : In this study, cell viability was confirmed by MTT assay. Production of TNF-α and IL-6 was determined by ELISA. mRNA expression of TARC and MDC were measusred by qRT-PCR. Also, expressions of p-JNK, JNK, p-ERK, ERK, p-p38, and p-38 were investigated by using western blot assay. Results : Aster glehni water extracts were not shown any significant cytotoxicity at 15.625-500㎍/㎖ in HaCaT keratinocytes. Aster glehni extracts inhibited the TNF-α and IL-6 production in HaCaT keratinocytes treated with TNF-α and IFN-γ. Also, expression of TARC, MDC, p-ERK, and p-STAT1 was decreased. Conclusions : These results suggest that Aster glehni water extracts have anti-inflammatory effects in HaCaT keratinocytes and can be applied to the development of anti-inflammatory treatment substances.

주제어

표/그림 (7)

표 Table 1. Sequences of the Real-Time Reverse Transcription Polymerase Chain Reaction(RT-PCR) Primers
그림 Fig. 1. Effects of A.glehni DW Extracts on Cell Viability in HaCaT Keratinocytes HaCaT cells were treated with various concentrations of A.glehni DW extracts for 24hrs, and their viability were determined by MTT assay. The data shown represent the mean±S.D. of three independent experiments. ***p<0.001 vs. A.glehni non-treated group.
그림 Fig. 2. Effects of A.glehni DW Extracts on Production and mRNA Expression of TNF-α in TNF-α, IFN-γ-stimulated HaCaT Keratinocytes HaCaT keratinocytes were treated with A.glehni DW extracts(125㎍/㎖, 250㎍/㎖, and 500㎍/㎖) for 1hr, and the cells were stimulated with TNF-α/IFN-γ(10ng/㎖) for (A) 24hrs and (B) 6hrs for cytokine production and detection of mRNA expression level, respectively. (A) Cytokine production of TNF-α was measured with ELISA kit. (B) The mRNA level of TNF-α was measusred by qRT-PCR. Values are the mean±S.D. of three independent experiments. ###p<0.001 vs. the control group; **p<0.01, and ***p<0.001 vs. TNF-α/IFN-γ-treated group; significances between treated groups were determined using ANOVA and Dunnett's post hoc test.
그림 Fig. 3. Effects of A.glehni DW Extracts on Production and mRNA Expression of IL-6 in TNF-α, IFN-γ -stimulated HaCaT Keratinocytes HaCaT keratinocytes were treated with A.glehni DW extracts(125㎍/㎖, 250㎍/㎖, and 500㎍/㎖) for 1hr. And the cells were stimulated with TNF-α/IFN-γ(10ng/㎖) for (A) 24hrs and (B) 6hrs for cytokine production and detection of mRNA expression level, respectively. (A) Cytokine production of IL-6 was measured with ELISA kit. (B) The mRNA level of IL-6 was measusred by qRT-PCR. Values are the mean±S.D. of three independent experiments. ^###p<0.001 vs. the control group; **p<0.01, and ***p<0.001 vs. TNF-α/IFN-γ-treated group; significances between treated groups were determined using ANOVA and Dunnett's post hoc test.
그림 Fig. 4. Effects of A.glehni DW Extracts on mRNA Expression of Th2-chemokines in TNF-α, IFN-γ -stimulated HaCaT Keratinocytes HaCaT keratinocytes were treated with A.glehni DW extracts(125㎍/㎖, 250㎍/㎖, and 500㎍/㎖) for 1hr. And the cells were stimulated with TNF-α/IFN-γ(10ng/㎖) for 6hrs for detection of mRNA expression. The relative mRNA expression of (A) TARC(CCL17) and (B) MDC(CCL22) were measusred by qRT-PCR. Values are the mean±S.D. of three independent experiments. ^##p<0.01 and ^###p<0.001 vs. the control group; *p<0.05, and **p<0.01 vs. TNF-α/IFN-γ-treated group; significances between treated groups were determined using ANOVA and Dunnett's post hoc test.
그림 Fig. 5. Effects of A.glehni DW Extracts on TNF-α, IFN-γ-stimulated Phosphorylation of MAPKs in HaCaT Keratinocytes HaCaT keratinocytes were treated with A.glehni DW extracts(125㎍/㎖, 250㎍/㎖, and 500㎍/㎖) for 1hr. And the cells were stimulated with TNF-α/IFN-γ(10ng/㎖) for 20 min and then their total proteins were extracted and western blotted for p-ERK, ERK, p-JNK, JNK, p-p38, p38, and β-actin. Each total form was used as internal control, respectivelyl. Densitometric analysis was performed using Bio-Rad Quantity One® software. Values are the mean±S.D. of three independent experiments. ^##p<0.01 and ^###p<0.001 vs. the control group; *p<0.05, **p<0.01, and ***p<0.001 vs. TNF-α/IFN-γ -treated group; significances between treated groups were determined using ANOVA and Dunnett's post hoc test.
그림 Fig. 6. Effects of A.glehni DW Extracts on TNF-α, IFN-γ-stimulated STAT1 Activation in HaCaT Keratinocytes HaCaT keratinocytes were treated with A.glehni DW extracts(125㎍/㎖, 250㎍/㎖, and 500㎍/㎖) for 1hr. And the cells were stimulated with TNF-α/IFN-γ(10ng/㎖) for 1hr. The total protein was extracted and western blotted for p-STAT1 and STAT1. β-actin was used as internal control. Densitometric analysis was performed using Bio-Rad Quantity One® software. Values are the mean±S.D. of three independent experiments. ^###p<0.001 vs. the control group; *p<0.05, and ***p<0.001 vs. TNF-α /IFN-γ-treated group; significances between treated groups were determined using ANOVA and Dunnett's post hoc test.

참고문헌 (34)

Spergel JM, Paller AS. Atopic dermatitis and the atopic march. Journal of Allergy and Clinical Immunology. 2003;112(6):S118-27.

상세보기
Leung DY, Bieber T. Atopic dermatitis. Lancet. 2003;361(9352):151-60.

상세보기
Howlett S. Emotional dysfunction, child-family relationships and childhood atopic dermatitis. British Journal of Dermatology. 1999;140(3):381-4.

상세보기
Foolad N, Brezinski EA, Chase EP, Armstrong AW. Effect of nutrient supplementation on atopic dermatitis in children: a systematic review of probiotics, prebiotics, formula, and fatty acids. JAMA Dermatology. 2013;149(3):350-5.

상세보기
Leung DY, Boguniewicz M, Howell MD, Nomura I, Hamid QA. New insights into atopic dermatitis. Journal of Clinical Investigation. 2004;113(5):651-7.

상세보기
Schultz-Larsen F, Hanifin JM. Epidermiology of atopic dermatitis. Immunology and Allergy Clinics of North America. 2002;22 (1):1-24.

상세보기
Leung DY. Atopic dermatitis: new insights and opportunities for therapeutic intervention. Journal of Allergy and Clinical Immunology. 2000;105(5):860-76.

상세보기
Mohan GC, Lio PA. Comparison of dermatology and allergy guidelines for atopic dermatitis management. JAMA Dermatology. 2015;151(9):1009-13.

상세보기
Lyons JJ, Milner JD, Stone KD. Atopic dermatitis in children: clinical features, pathophysiology, and treatment. Immunology and Allergy Clinics of North America. 2015;35(1):161-83.

상세보기
Eun SY, Yoon JJ, Kim HY. Protective Effects of Chijabaegpi-tang on Atopic Dermatitis in TNF-α/IFNγ-induced HaCaT Cells. Journal of Physiology & Pathology in Korean Medicine. 2018;32(4):226-31.

원문보기 상세보기
Lee TB. Coloured flora of Korea. Seoul:Hyang moon sa. 2006:322.
Jung SI, Choi BM, Yoon YG, Lee JW, Jang SI. A Noble Therapeutic Approach of Atopic dermatitisby Development of Th2 Chemokine Inhibitors from Natural Products: Inhibitory Effect of Sophora flavescens Extract Atopic Dermatitis Model mice, NC/Nga. Herbal Formula Science. 2009;17(1):1218-29.
Kim CM, Sin MK, An TK, Lee KS. Dictionary of Chinese Herb. Seoul:JungDam Publisher. 1997:1431.
Lee JY, Park JY, Kim HD, Lee SE, Lee JH, Lee YJ, et al. Anti-oxidant and anti-adipocyte differentiation of Aster glehni and Aster yomena. Journal of Nutrition and Health. 2019;52(3):250-7.

원문보기 상세보기
Cho MJ. A study on the antioxidant nutrients analysis and antioxidative activities of Adenophora remotiflora and Aster glehni. Duksung women's Univ Masters degree thesis. 2001:1-50.
Sim JH, Lee HS, Lee S, Park DE, Oh K, Hwang KA, et al. Anti-asthmatic activities of an ethanol extract of Aster yomena in an ovalbumin-induced murine asthma model. Journal of Medicinal Food. 2014;17(5): 606-11.

원문보기 상세보기
Kim HH, Park GH, Park KS, Lee JY, Kim TH, An BJ. The Effect of Aster glehni Fr. Schm. Extracts on Whitening and Anti-Wrinkle. Department of Cosmeceutical Science. Journal of Life Science. 2010;20 (7):1034-40.

원문보기 상세보기
Rollins BJ, Yoshimura T, Leonard EJ, Pober JS. Cytokine-activated human endothelial cells synthesize and secrete a monocyte chemoattractant, MCP-1/JE. The American Journal of Pathology. 1990;136(6):1229-33.

상세보기
Ko HG, Lee KY, Hong CH. Anti-inflammatory Effects of Aster glehni Water Extracts in LPS-stimulated RAW 264.7 Macrophages. J Korean Med Ophthalmol Otolaryngol Dermatol. 2022;35(1):1-10.
Bae GS, Park SJ. Anti-inflammatory Effect of Nypa fruticans Wurmb. on tumor necrosis factor(TNF)-α-induced Inflammatory response in HaCaT cells. The Korea Journal of Herbology. 2019;34(1):51-7.

원문보기 상세보기
Kang GJ, Kang NJ, Han SC, Koo DH, Kim YS, Lee JH, et al. Inhibitory Effect of Artemisinic Acid Isolated from Artemisia Annua Lon the MDC in HaCaT Keratinocytes. Korean Journal of Pharmacognosy. 2012; 43(3):217-23.
Pivarcsi A, Nagy I, Kemeny L. Innate Immunity in the Skin: How Keratinocytes Fight Against Pathogens. Current Immunology Reviews. 2005;1(1):29-42.
Homey B, Steinhoff M, Ruzicka T, Leung DY. Cytokines and chemokines orchestrate atopic skin inflammation. Journal of Allergy and Clinical Immunology. 2006;118(1): 178-89.

상세보기
Abramovits W. Atopic dermatitis. Journal of the American Academy of Dermatology. 2005;53(1):S86-93.

상세보기
Bonness S, Bieber, T. Molecular basis of atopic dermatitis. Current Opinion in Allergy and Clinical Immunology. 2007;7 (5):382-6.

상세보기
Lee MS, Jung MS. Anaysis of Volatile Flavor Components of Aster glehni. Korean Society of Food and Cookery Science. 1998;14 (5):547,548.
Min YD, Kwon HC, Choi SZ, Lee KR. Terpenoids from the aerial parts of Aster glehni. YAKHAK HOEJI. 2004;48(1):65-9.
Kim KW, Shin JG, Lee DG. Isolation of herbicidal compound from Aster glehni Fr. Schm. Korean Journal of Weed Science. 2008;28(4):434-41.
Nam YK, Baik JA. Status of research and possibility of development about endemic wild vegetables in Korea. Korean Society for People, Plant, and Environment. 2005;8: 1-10.
Kang NL, Han EJ, Park SY, Jee Y, Jeon YJ, Ahn CB. Inhibitory Effects of Ecklonia cava Ethanol Extracts against TNF-α/IFN-γ Induced Inflammation in Human Keratinocytes. Journal of Chitin and Chitosan. 2017;22(2):82-8.

상세보기
Pivarcsi A, Homey B. Chemokine networks in atopic dermatitis : traffic signals of disease. Current Allergy and Asthma Reports. 2005;5(4):284-90.

상세보기
Hong JS, Koh JM, Kim SW, An CS, Kim DK, Kim GS. Intracellular Signal Transduction Pathway of Leptin-Induced Apoptosis in Human Bone Marrow Stromal Cells: ERK/cPLA2 and JAK-STAT1. Journal of bone metabolism. 2003;10(2):143-51.
Madhani HD, Styles CA, Fink GR. MAP kinases with distinct inhibitory functions impart signaling specificity during yeast differentiation. Cell. 1997;91(5):673-84.

상세보기
Hagemann C, Blank JL. The ups and downs of MEK kinase interactions. Cell Signal. 2001:13(12):863-75.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증