[논문]TNF-α/IFN-γ 유도된 각질형성세포 염증에서 가자 추출물의 케모카인 저해 효과

조일주

doi:10.6116/kjh.2022.37.3.41.

TNF-α/IFN-γ 유도된 각질형성세포 염증에서 가자 추출물의 케모카인 저해 효과
Inhibitory activity of Terminalia chebula extract against TNF-α/IFN-γ-induced chemokine increase on human keratinocyte, HaCaT cells 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.37 no.3, 2022년, pp.41 - 47

조일주 (원광대학교 한의과대학 뇌졸중한의중점연구센터)

Abstract ▼ AI-Helper

Objectives : Terminalia chebula (TC) has been used as a traditional remedy to treat gastrointestinal infectious and inflammatory diseases. However, its protective effects and mechanisms against skin inflammation have not been well-elucidated. Thus, the aim of this study is to evaluate the protective effects of the TC water extract and also to suggest a putative mechanism of TC against skin injury on human keratinocytes, HaCaT cells. Methods : HaCaT cells were pre-treated with TC for 1 h and then stimulated with tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) (10 ng/mL each) to induce skin inflammation and injury. After 24 h, the cells were harvested to evaluate the expression of Th2 chemokines, such as C-C motif chemokine ligand 5 (CCL5, also known as RANTES), C-C chemokine ligand 17 (CCL17, also known as TARC) and C-C chemokine ligand 22 (CCL22, also known as MDC). To investigate the regulatory mechanisms of TC, we also assessed the phosphorylation of signal transducer and activator of transcription 1 (STAT1) signaling pathways in HaCaT cells. Results : Treatment of TC decreased the mRNA levels of RANTES, TARC and MDC with a concentration dependent manner against co-stimulation of TNF-α and IFN-γ. In addition, TC significantly reduced TNF-α and IFN-γ induced phosphorylation of STAT1. Conclusions : In summary, we propose that TC may be a promising candidate for anti-inflammatory skin protector through the inhibition of chemokines via STAT1 deactivation.

주제어

표/그림 (5)

그림 Fig. 1. Cytotoxic effects of Terminalia chebula (TC) in HaCaT cells. The cell viability was measured by MTT assay. HaCaT cells were incubated with or without TC as indicated doses (0.05, 0.1, 1, 2, 5, 10, 20 or 50 mg/mL) for 24 h. #p < 0.05 versus normal. The values are means ± S.D. of three independent experiments.
표 Table 1. Sequences of realtime RT-PCR primers.
그림 Fig. 2. Effects of TC on mRNA expression of RANTES chemokine in HaCaT cells. HaCaT cells were pre-treated with TC (1, 2 or 5 mg/mL) for 1 h and incubation with TNF-α and IFN-γ (10 ng/mL) for 24 h. The mRNA levels of RANTES were measured by realtime RT-PCR. ^#p < 0.05 versus normal. ⁎p < 0.05 versus TNF-α + IFN-γ alone. The values are means ± S.D. of three independent experiments.
그림 Fig. 3. Effects of TC on mRNA expression of TARC and MDC chemokines in HaCaT cells. HaCaT cells were pre-treated with TC (1, 2 or 5 mg/mL) for 1 h and incubation with TNF-α and IFN-γ (10 ng/mL) for 24 h. The mRNA levels of TARC and MDC were measured by realtime RT-PCR. ^#p < 0.05 versus normal. ⁎p < 0.05 versus TNF-α + IFN-γ alone. The values are means ± S.D. of three independent experiments.
그림 Fig. 4. Effects of TC on TNF-α and IFN-γ induced STAT1 phosphorylation in HaCaT cells. HaCaT cells were pre-treated with TC (1, 2 or 5 mg/mL) for 1 h and incubation with TNF-α and IFN-γ (10 ng/mL) for 15 min. Phosphorylated and total expression of STAT1 and β-actin were detected by Western blot analysis using specific antibodies. The similar results were obtained from three additional experiments.

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