[논문]갈산과 타닌산의 자동산화에 미치는 식품 감광성분의 영향

이은빈; 이효원; 홍정일

doi:10.9721/kjfst.2022.54.3.272

갈산과 타닌산의 자동산화에 미치는 식품 감광성분의 영향
The effects of dietary photosensitizers on auto-oxidation of gallic and tannic acids 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.54 no.3, 2022년, pp.272 - 279

이은빈 (서울여자대학교 자연과학대학 식품응용시스템학부) , 이효원 (서울여자대학교 자연과학대학 식품응용시스템학부) , 홍정일 (서울여자대학교 자연과학대학 식품응용시스템학부)

초록
AI-Helper

본 연구에서는 감광제에 의한 폴리페놀의 갈변 정도 및 폴리페놀 함량의 변화와 산화방지활성과 ROS 생성을 분석하였다. 그 결과 감광제에 의해 갈산과 타닌산의 자동산화가 현저히 가속화 되었으며, 타닌산에서 그 효과가 더욱 두드러졌다. 폴리페놀 함량은 산화가 진행됨에 따라 유의적으로 감소하였으나, 감광제에 농도에 의한 유의적인 차이는 EB에 의해서만 나타났으며, ABTS 라디칼 소거능 변화에서도 유사한 양상을 보였다. 갈산과 타닌산에서 ROS 생성의 증가는 명소에서 감광제 존재 하에 농도 유의적으로 증가하였다. 본 연구 결과는 폴리페놀이 감광제와 함께 빛에 노출 시 폴리페놀의 산화를 촉진시키며, 식품의 가공 및 저장 시 이들의 상호작용을 통한 품질저하 및 생리활성 변화에 주의할 필요가 있음을 시사한다.

Abstract ▼ AI-Helper

Polyphenols are chemically unstable, and their bioactivities are reduced through oxidation. Photosensitizers (PS) induce photo-oxidation in various food systems. In this study, effects of dietary PS such as riboflavin (Rb), erythrosin B (EB), and zinc protoporphyrin on the auto-oxidation of polyphenols, gallic acid (GA) and tannic acid (TA) were evaluated under a fluorescent light. The formation of oxidation products from GA and TA increased in a PS concentration- and irradiation time-dependent manner. In addition, Rb and EB induced significant reduction in the polyphenols contents and ABTS radical scavenging activity of GA and TA under light. PS significantly enhanced the amount of reactive oxygen species generated from GA and TA. Therefore, the interaction of polyphenols with PS under light results in acceleration of polyphenol oxidation. This phenomenon should be carefully considered during food processing and storage.

주제어

표/그림 (6)

그림 Fig. 1. Changes in absorbance spectra of gallic acid or tannic acid incubated with erythrosin B under light. Absorbance spectra of 2 mM gallic acid (GA) (A) and 200 μM tannic acid (TA) (B) incubated with 4 μM erythrosin B (EB) dissolved in PBS (pH 7.4) under fluorescent light irradiation for 4 h were analyzed within 400-700 nm.
그림 Fig. 2. Effects of different photosensitizers on gallic acid oxidation under light. Gallic acid oxidation was induced in PBS (pH 7.4) in the absence or presence of different concentrations of Rb (A), EB (B), and ZnPP (C) under a fluorescent light or in a dark (D). The formation of oxidation products was analyzed at absorbance at 405 nm, and half-saturation time (the time required for 50% maximum absorbance of oxidation products, ST₅₀) were also calculated (E). Each value represents the mean±SD (n=4). Different letters indicate significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test.
그림 Fig. 3. Effects of different photosensitizers on tannic acid oxidation under light. Gallic acid oxidation was induced in PBS (pH 7.4) in the absence or presence of different concentrations of Rb (A), EB (B), and ZnPP (C) under a fluorescent light or in a dark (D). The formation of oxidation products was analyzed at absorbance at 405 nm, and half-saturation time (ST₅₀) were also calculated (E). Each value represents the mean±SD (n=4). Different letters indicate significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test.
표 Table 1. Changes in total polyphenol content of gallic and tannic acids incubated with different photosensitizers under light or in a dark condition for 0, 4, and 24 h
표 Table 2. Changes in ABTS scavenging activities of gallic and tannic acids incubated with different photosensitizers under light or in a dark condition for 0, 4, and 24 h
그림 Fig. 4. Effects of different photosensitizers on hydrogen peroxide formation from gallic acids and tannic acid under light or in a dark. Gallic (A and B) and tannic (C and D) acids were incubated with different concentrations of photosensitizers for 4 h under light (A and C) or in a dark (B and D), and formation of hydrogen peroxide was analyzed. Each value represents the mean±SD (n=3). Different letters indicate significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test.

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