[논문]유산균 생산 후 폐기되는 부산물 첨가 사료의 급이가 잉어(Cyprinus carpio)의 성장률, 선천성 면역, 면역연관 유전자 발현 및 항균효과에 미치는 영향

최재혁; 정상목; 양은총; 장태원; 이찬흔; 박관하; 최상훈

doi:10.7847/jfp.2022.35.1.103

유산균 생산 후 폐기되는 부산물 첨가 사료의 급이가 잉어(Cyprinus carpio)의 성장률, 선천성 면역, 면역연관 유전자 발현 및 항균효과에 미치는 영향
Effects of dietary by-products discarded after probiotics production (BPPP) on growth performance, innate immunity, immune gene expression, and disease resistance against Edwardsiella tarda in carp, Cyprinus carpio 원문보기

韓國魚病學會誌= Journal of fish pathology, v.35 no.1, 2022년, pp.103 - 111

최재혁 (군산대학교 해양과학대학 수산생명의학과) , 정상목 (군산대학교 해양과학대학 수산생명의학과) , 양은총 (군산대학교 해양과학대학 수산생명의학과) , 장태원 (군산대학교 해양과학대학 수산생명의학과) , 이찬흔 ((유)금성상공) , 박관하 (군산대학교 해양과학대학 수산생명의학과) , 최상훈 (군산대학교 해양과학대학 수산생명의학과)

Abstract ▼ AI-Helper

This study has been performed to investigate the potential effects of by-product discarded after probiotics production (BPPP) on growth performance, immune gene expression, innate-immunity status, and disease resistance of carp, Cyprinus carpio. For 3 weeks, carp were fed four diets containing different levels of BPPP at 0, 0.1, 0.2 and 0.5% per kg of normal diet. Every 7 days of feeding, immune-related gene expression, serum lysozyme activity and ACH50 were analyzed. Growth rates and challenge test with E. tarda were conducted after 3 weeks of BPPP feeding. Both lysozyme activity and ACH50 were significantly (p<0.05) increased in all BPPP supplemented groups compared to the control at every 7 day for 3 weeks of feeding trial. The gene expression of pro-inflammatory cytokines, IL-1β and TNF-α was significantly (p<0.05) up-regulated until 21 days of feeding in all groups except for 0.2% group on day 7 post feeding. The anti-inflammatory cytokine IL-10 gene expression was only significantly (p<0.05) increased in 0.1% group on day 7 and decreased (p<0.05) on day 14 in all BPPP supplemented groups. On day 21, the IL-10 gene expression was augmented (p<0.05) in all groups. SOD gene expression was significantly (p<0.05) increased compared to the control on day 14 and 21 post feeding, whereas no significant difference was observed on day 7. In challenging test, 0.2%, 0.1%, 0.5% and control group showed 80%, 70%, 60% and 40% of survival rate, respectively. Feed conversion rate was only improved in 0.5% group. In conclusion, the present study indicates that dietary BPPP suplementation improved growth performance, innate immune response and bactericidal activity in carp.

주제어

표/그림 (5)

표 Table 1. Primers used for amplification of specific transcripts by quantitative PCR
그림 Fig. 1. Effects of dietary BPPP on lysozyme activity (A) and ACH₅₀ (B) of carp. Data represent the mean±S.D. (n=5). Different letters above the bars indicate significant differences (p<0.05) in different groups.
그림 Fig. 2. Effects of BPPP on relative mRNA gene expression of IL-1β and TNF-α, IL-10 and SOD in carp. Data represent the mean±S.D. (n=5). Different letters above the bars indicate significant differences (p<0.05) in different groups.
그림 Fig. 3. Relative percent survival of carp for 10 days following Edwardsiella tarda (2×10⁸ CFU/ml) challenge. Carp were fed 0.1, 0.2 and 0.5% BPPP added diet, respectively for 21 days followed by i. p. injection with E. tarda. Normal diet was fed as a control.
표 Table 2. Growth performances and feed utilization of carp fed different levels of dietary BPPP.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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