[논문]수입 냉동새우에서 검출된 YHV3와 IHHNV의 계통학 및 병원성 분석

백은진; 정예진; 정민아; 박지연; 김광일

doi:10.7847/jfp.2022.35.1.027

수입 냉동새우에서 검출된 YHV3와 IHHNV의 계통학 및 병원성 분석
Phylogenetic and pathogenic traits of YHV3 and IHHNV detected from imported frozen shrimp 원문보기

韓國魚病學會誌= Journal of fish pathology, v.35 no.1, 2022년, pp.27 - 40

백은진 (부경대학교 수산생명의학과) , 정예진 (부경대학교 수산생명의학과) , 정민아 (부경대학교 수산생명의학과) , 박지연 (부경대학교 수산생명의학과) , 김광일 (부경대학교 수산생명의학과)

초록
AI-Helper

2019년부터 2020년도까지 국내로 수입 및 유통되고 있는 냉동 새우에 대하여 갑각류 전염병인 전염성피하및조혈기괴사증(infectious hypodermal andhematopoietic necrosis, IHHN), 노란머리병(yellow head disease, YHD), 타우라증후군(taura syndrome, TS), 전염성근괴사증(infectious myonecrosis, IMN)의 병원체 유전자 검출 여부를 조사하였다. Polymerase chain reaction (PCR) 분석을 수행한 결과 5개 국가에서 수입된 총 10개 그룹의 시료에서 YHV (1/10), IHHNV (2/10)가 검출되었으며 TSV와 IMNV는 검출되지 않았다. 또한, 검출된 IHHNV의 경우 type A IHHNV related gene을 증폭시킬 수 있는 MG831F/R primer를 이용한 PCR 결과에서도 양성으로 확인되었다. 유전자가 검출된 시료에 대해 염기서열 및 계통학적 분석 결과 YHV는 genotype 3 (YHV3)로 분류되었으며 IHHNV는 infectious IHHNV type 2 로 분류되었다. PCR 양성 조직 시료를 마쇄 후 흰다리새우에 인위 감염 실험을 수행한 결과, 폐사는 나타나지 않았으나 접종한 바이러스 유전자는 검출되었다. 이러한 결과는 YHV3와 IHHNV가 냉동 새우의 수입 과정에서 국내로 유입되고 있음을 시사한다. 본 연구에서 검출된 YHV3와 IHHNV는 병원성이 없는 유전형 또는 수입 및 유통 과정에서 불활화에 따라 흰다리새우에 폐사를 유발하기에는 감염성이 낮은 수준의 형태로 존재하고 있을 것으로 사료된다.

Abstract ▼ AI-Helper

Yellow head virus (YHV), Infectious hypodermal and hematopoietic necrosis (IHHNV), Taura syndrome virus (TSV), and Infectious myositis virus (IMNV) cause serious mortality to Penaeidae shrimp in the aquaculture. In this study, YHV, IHHNV, TSV, and IMNV were surveyed from imported frozen shrimps between 2019 and 2020 via molecular diagnostic assay. Among 10 shrimp groups, YHV (n=1) and IHHNV (n=4) were detected by RT-PCR and PCR, respectively. From the phylogenetic analysis based on the partial ORF 1b region of YHV, YHV was classified into YHV genotype 3 (YHV3). And IHHNVs (n=2) detected from Litopenaeus vannamei belong to infectious IHHNV type 2. Although IHHNVs (n=2) identified from Penaeus monodon showed PCR positive results (MG 831F/R primer set), the sequences of ORF 2 and 3 were not amplified, suggesting that those samples might possess type A IHHNV related sequence of P. monodon. Furthermore, in the challenge test, even though PCR-detected isolates (YHV3/type A IHHNV related sequence or infectious IHHNV type 2) were not induced mortality to L. vannamei, viral genes were amplified suggesting that the viruses in the frozen shrimp could be non-pathogenic particles which are not enough to induce mortality.

주제어

표/그림 (6)

표 Table 1. PCR primers used in this study
표 Table 1. Continued
표 Table 2. The results of monitoring from imported frozen shrimp between 2019 and 2020
그림 Fig. 1. Phylogenetic tree of YHV genotype 1 to 8 based on nucleotide sequence of the partial YHV ORF 1b region retrieved from GeneBank. The distinct genotype of YHV determined by Maximum likelihood analysis using Mega (ver 11.0.10) software. The numbers indicate the percentages of bootstrap support from 1000 replicates.
그림 Fig. 2. Phylogenetic tree based on nucleotide sequence of the IHHNV ORF 2 retrieved from GeneBank. The distinct geographical isolates of IHHNV determined by Maximum likelihood analysis using MEGA (ver 11.0.10) software. The numbers indicate the percentages of bootstrap support from 1000 replicates.
그림 Fig. 3. Phylogenetic tree based on nucleotide sequence of the IHHNV ORF 3 retrieved from GeneBank. The distinct geographical isolates of IHHNV determined by Maximum likelihood analysis using MEGA (ver 11.0.10) software. The numbers indicate the percentages of bootstrap support from 1000 replicates.

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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