[논문]포르말린과 청색 LED 병용처리에 의한 항스쿠티카 효과

강문경; 이주엽; 이윤항; 김도형

doi:10.7847/jfp.2022.35.1.113

포르말린과 청색 LED 병용처리에 의한 항스쿠티카 효과
Anti-scuticociliate effects of a combined treatment with formalin and blue LED 원문보기

韓國魚病學會誌= Journal of fish pathology, v.35 no.1, 2022년, pp.113 - 120

강문경 (부경대학교 수산생명의학과) , 이주엽 (부경대학교 수산생명의학과) , 이윤항 (부경대학교 수산생명의학과) , 김도형 (부경대학교 수산생명의학과)

Abstract ▼ AI-Helper

Scuticociliatosis caused by Miamiensis avidus is a very important parasitic disease in olive flounder farming industry. The aim of this study was to determine effect of combined treatment with blue LED (light-emitting diode) illumination and formalin on olive flounder (Paralichthys olivaceus) infected with M. avidus. Different intensity of 405 nm LED (20, 40, and 60 μmol·m-2·s-1) was illuminated on 2.2×104 cells/well of M. avidus in a 24 well microplate for 24 h. Also, 2.4×104 cells/well of M. avidus were exposed to varying combinations of 60 μmol·m-2·s-1 of 405 nm LED and serial 10-fold dilutions of formalin (from 10 to 100 ppm) for 15, 30, 45, and 60 min. Surviving M. avidus were counted using a hemocytometer. For in vivo test, flounder acclimatized at 11-12 practical salinity unit (psu) were challenged with 2×106 cells/ml of M. avidus by immersion method for 1 h. Then, fish were moved and divided into four groups; "F" group, treated with formalin at 50 ppm; "L" group, treated with 60 μmol·m-2·s-1 of 405 nm LED; "C" group, treated with combination of the two methods; and the control group. After treatment for 30 min, fish were transferred to new tanks (salinity = 11-12 psu) and observed for 3 weeks. As a result, illumination of 405 nm LED at 60 μmol·m-2·s-1 killed 100% of M. avidus after 12 h, while 67% and 90% of the scuticociliate died at 20 and 40 μmol·m-2·s-1, respectively, after 24 h exposure. One hundred percent of M. avidus was killed at 90, 80, 80 and 70 ppm after exposure to formalin for 15, 30, 45 and 60 min, respectively. However, combined method (e.g., 60 μmol·m-2·s-1 of 405 nm-LED plus 50 ppm formalin) killed the parasite within 30 min. From in vivo test, similarly, survival rates of fish challenged with M. avidus were 100%, 43%, 29% and 0% in the C, F, L, and control groups, respectively. Results obtained in this study demonstrates that the combined treatment method has clear synergistic effect on scuticociliatosis in fish.

주제어

표/그림 (5)

그림 Fig. 1 LED wavelength range of 405 nm LED.
그림 Fig. 2. Viable M. avidus counts after exposure to various intensity of 405 nm LED. Ambient light: control, 20 μmol·m^-2·s^-1 : 20 μmol·m^-2·s^-1 of 405 nm LED, 40 μmol·m^-2·s-1: 40 μmol· m^-2·s^-1 of 405 nm LED, 60 μmol·m^-2· s^-1: 60 μmol·m^-2·s^-1 of 405 nm LED.
그림 Fig. 3. Reduction rate of M. avidus after exposure to various combinations of 60 μmol·m^-2·s^-1 405 nm LED with serial dilutions of formalin (from 10 to 100 ppm) and treatment of serial dilutions of formalin only (from 10 to 100 ppm) for (15 minutes, (A), 30 minutes (B), 45 minutes (C) and 60 minutes (D). *, Significant difference, P < 0.05. **, Significant difference, P < 0.01 and ***, Significant difference, P < 0.001.
그림 Fig. 4. Cumulative survival rate (%) of olive flounder after M. avidus immersion challenge; Formalin-treated group (formalin at 50 ppm), LEDtreated group (60 μmol·m^-2·s^-1 of 405 nm LED), Combined LED- and formalin- treated group (Formalin at 50 ppm+60 μmol·m^-2·s^-1 of 405 nm LED) and Control group.
표 Table 1. Reduction rate (%) of M. avidus according to exposure time of various concentrations of formalin (10 to 100 ppm) with 405 nm LED (60 μmol·m^-2·s^-1)

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