[논문]Balb/c 생쥐에 대한 어싱 매트리스에 의한 항염 효과

김지연

doi:10.15188/kjopp.2022.06.36.3.89

Balb/c 생쥐에 대한 어싱 매트리스에 의한 항염 효과
Anti-inflammatory Effects of Earthing Mattress in Mouse 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.36 no.3, 2022년, pp.89 - 93

김지연 ((주)월드홈닥터 & 아주대학교 글로벌제약임상대학원 의약전공)

Abstract ▼ AI-Helper

Earthing, caused by direct skin contact with the Earth's surface, is used to reduce the symptoms of inflammation (fever, fever, swelling and pain). However, there is little evidence to support the anti-inflammatory effects of earthing mattresses. Therefore, this study was conducted to investigate whether anti-inflammatory effect of earthing mattress using an in vivo animal model. The anti - inflammatory effect was evaluated by measuring ear thickness and foot volume in 12-O-tetradecanoylphorbol-13 acetate (TPA) - induced ear edema and carrageenan - induced paw edema model, respectively. Balb/c mouse in carrageenan paw edema model showed significant anti - inflammatory effect in the group treated with earthing mattress for 4 hours or 24 hours for 3 days. For females, the anti-inflammatory effect was greater when the earthing mattress was added to the mattress than the mattress alone treatment. From the above results, it was found that the female responds more to the effect of the earthing as well as the mattress effect. In addition, when the male and female Balb/c mice were exposed to mattresses and earthing mattresses for 24 h for 3 days, respectively, the mattress and earthing mattresses showed significant inhibition of IL (Interleukin)-1β levels compared to the control. In the TPA ear edema model, Balb/c mouse showed significant anti - inflammatory effect in the group treated with the earthing mattress for 4 hours or 24 hours for 3 days. Both males and females showed more anti-inflammatory effects when they were exposed to earthing mattresses with mattresses added to the mattresses. From the above results, it was found that both male and female respond to the effect of earthing as well as the mattress effect in the TPA ear edema model. In conclusion, in this study, we have verified that earthing mattress shows inhibitory effects on TPA and carrageenan-induced inflammation. From these results, it is suggested that the anti-inflammatory effect can be expected by applying the earthing mattress to patients suffering from inflammatory diseases. However, there is a need to pinpoint exactly how the earthing mattress relieves inflammation, and further research is needed to investigate the mechanism.

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표/그림 (8)

그림 Fig. 1. Inflammation levels in the male mouse exposed to Earthing mattress for 1h/day, 2h/day, 4h/day, 6h/day for 3 days. Fig1A After exposed to Earthing mattress for 1h/day for 3 days. Fig 1B After exposed to Earthing mattress for 2h/day for 3 days. Fig 1C After exposed to Earthing mattress for 4h/day for 3 days. Fig 1D After exposed to Earthing mattress for 6h/day for 3 days. λ-Carrageenan at 1% was injected into the hindpaw, causing acute inflammation. Respectively, average of each group converted to % control and result represent the mean ± S.E.M. (n=5) ** p < 0.01 vs control.
그림 Fig. 2. Inflammation levels in the Carrageenan injected mouse exposed to Earthing mattress for 4h/day. Anti-inflammation effect of Earthing mattress in the Carrageenan induced mouse inflammation. Fig 3A Male mouse was exposed to the Earthing mattress 4h/day for 3days. Fig 3B Male mouse was exposed to the Earthing mattress for 4h/day for 5 days. Fig 3C Female mouse was exposed to the Earthing mattress for 4h/day for 3days. Fig 4D Female mouse was exposed to the Earthing mattress for 4h/day for 5days. Fig 4E Female mouse was exposed to the Earthing mattress for 4h/day for 7days. After that, λ-Carrageenan at 1% was injected into the hindpaw, causing acute inflammation. Respectively, average of each group converted to % control and result represent the mean ± S.E.M (n=5) * p < 0.05 vs controls, ** p < 0.01 vs control.
그림 Fig. 3. Inflammation levels in Carrageenan injected mouse exposed to Earthing mattress for 24h/day. Fig 4A Male mouse was exposed to the Earthing mattress for 24h/day for 3 days. Fig 4B Female mouse was exposed to the Earthing mattress for 4h/day for 3 days. After that λ -Carrageenan at 1% was injected into the hindpaw, causing acute inflammation. Respectively, average of each group converted to % control and result represent the mean ± S.E.M. (n=5) ** p < 0.01 vs control.
그림 Fig. 4. Inflammation levels in Carrageenan injected male mouse and female mouse exposed to mattress or Earthing mattress for 24h/day. Fig 5A Male mouse was exposed to the mattress or Earthing mattress for 4h/day for 3 days. Fig 5B Female mouse was exposed to the mattress or Earthing mattress for 4h/day for 3 days. After that λ-Carrageenan at 1% was injected into the hindpaw, causing acute inflammation. Respectively, average of each group converted to % control and result represent the mean ± S.E.M. (n=5) * p < 0.05 vs controls, ** p < 0.01 vs control.
그림 Fig. 5. Changes of cytokine levels in the Carrageenan injected male mouse and female mouse exposed to Earthing mattress for 24h/day. for 3 days Fig 6A Inflammatory male mouse model induced to Carrageenan. Fig 6B Inflammatory female mouse model induced to Carrageenan. Respectively, after exposed to Earthing mattress for 24h/day for 3days, used to IL-1β kit. Average of each group converted to % control and result represent the mean ± S.E.M. (n=5) * p < 0.05 vs controls.
그림 Fig. 6. Inflammation levels in the TPA injected male mouse and female mouse exposed to Earthing mattress for 4h/day. Fig 8A Male mouse was exposed to the Earthing mattress for 4h/day for 3days. Fig 8B Male mouse was exposed to the Earthing mattress for 4h/day for 5days. Fig 8C Female mouse was exposed to the Earthing mattress for 4h/day for 3days. Fig 8D Female mouse was exposed to the Earthing mattress for 4h/day for 5days. Mouse was induced to ear edema on using TPA. Average of each group converted to % control and result represent the mean ± S.E.M. (n=5) * p < 0.05 vs controls, ** p < 0.01 vs control.
그림 Fig. 7. Inflammatory levels in the TPA injected male mouse and female mouse exposed to Earthing mattress for 24h/day. Fig 9A Male mouse was exposed to Earthing mattress for 24h/day for 3 days or 5 days. Fig 9B Female mouse was exposed to Earthing mattress for 24h/day for 3 days or 5 days. Mouse was induced to ear edema on using TPA. Average of each group converted to % control and result represent the mean ± S.E.M. (n=5) * p < 0.05 vs controls, ** p < 0.01 vs control.
그림 Fig. 8. Inflammatory levels in the TPA injected male mouse and female mouse exposed to mattress or Earthing mattress for 24h/day. Fig 10A Male mouse was exposed to the mattress or Earthing mattress for 24h/day for 3 days or 5 days. Fig 10B Female mouse was exposed to the mattress or Earthing mattress for 24h/day for 3 days or 5 days. Mouse was induced to ear edema on using TPA. Average of each group converted to % control and result represent the mean ± S.E.M. (n=5) * p < 0.05 vs controls, ** p < 0.01 vs control.

참고문헌 (25)

Ferrero-Miliani L, Nielsen OH, Andersen PS, Girardin SE. Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin Exp Immunol. 2007;147(2):227-35.
Medzhitov R. Origin and physiological roles of inflammation. Nature. 2008;454(7203):428-35.

상세보기
Barton GM. A calculated response: control of inflammation by the innate immune system. J Clin Invest. 2008;118(2):413-20.

상세보기
Chevalier G, Sinatra ST, Oschman JL, Sokal K, Sokal P. Earthing: health implications of reconnecting the human body to the Earth's surface electrons. J Environ Public Health. 2012;2012:291541.
Menigoz W, Latz TT, Ely RA, Kamei C, Melvin G, Sinatra D. Integrative and lifestyle medicine strategies should include Earthing (grounding): Review of research evidence and clinical observations. Explore (NY). 2020;16(3):152-60.
Borthakur A, Bhattacharyya S, Dudeja PK, Tobacman JK. Carrageenan induces interleukin-8 production through distinct Bcl10 pathway in normal human colonic epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2007;292(3):G829-38.
Tobacman JK. Review of harmful gastrointestinal effects of carrageenan in animal experiments. Environ Health Perspect. 2001;109(10):983-94.

상세보기
Menegazzi M, Di Paola R, Mazzon E, Muia C, Genovese T, Crisafulli C, et al. Hypericum perforatum attenuates the development of carrageenan-induced lung injury in mice. Free Radic Biol Med. 2006;40(5):740-53.

상세보기
Ahmad TB, Liu L, Kotiw M, Benkendorff K. Review of anti-inflammatory, immune-modulatory and wound healing properties of molluscs. J Ethnopharmacol. 2018;210:156-78.

상세보기
Watanabe K, Reddy BS, Wong CQ, Weisburger JH. Effect of dietary undegraded carrageenan on colon carcinogenesis in F344 rats treated with azoxymethane or methylnitrosourea. Cancer Res. 1978;38(12):4427-30.

상세보기
Onderdonk AB. The carrageenan model for experimental ulcerative colitis. Prog Clin Biol Res. 1985;186:237-45.

상세보기
Moyana TN, Lalonde JM. Carrageenan-induced intestinal injury in the rat--a model for inflammatory bowel disease. Ann Clin Lab Sci. 1990;20(6):420-6.

상세보기
Nacife VP, Soeiro MD, Araujo-Jorge TC, Castro-Faria Neto HC, Meirelles MD. Ultrastructural, immunocytochemical and flow cytometry study of mouse peritoneal cells stimulated with carrageenan. Cell Struct Funct. 2000;25(6):337-50.

상세보기
Cuzzocrea S, Mazzon E, Dugo L, Serraino I, Ciccolo A, Centorrino T, et al. Protective effects of n-acetylcysteine on lung injury and red blood cell modification induced by carrageenan in the rat. Faseb j. 2001;15(7):1187-200.

상세보기
Redondo P, Garcia-Foncillas J, Espana A, Cuevillas F, Quintanilla E. Differential modulation of IL-8 and TNF-alpha expression in human keratinocytes by buflomedil chlorhydrate and pentoxifylline. Exp Dermatol. 1997;6(4):186-94.

상세보기
Xian YF, Lin ZX, Xu XY, Su ZR, Chen JN, Lai XP, et al. Effect of Rhizoma Polygonati on 12-O-tetradecanoylphorbol-acetate-induced ear edema in mice. J Ethnopharmacol. 2012;142(3):851-6.

상세보기
Wilches I, Tobar V, Penaherrera E, Cuzco N, Jerves L, Vander Heyden Y, et al. Evaluation of anti-inflammatory activity of the methanolic extract from Jungia rugosa leaves in rodents. J Ethnopharmacol. 2015;173:166-71.

상세보기
Maurya AK, Mohanty S, Pal A, Chanotiya CS, Bawankule DU. The essential oil from Citrus limetta Risso peels alleviates skin inflammation: In-vitro and in-vivo study. J Ethnopharmacol. 2018;212:86-94.

상세보기
Rao TS, Currie JL, Shaffer AF, Isakson PC. Comparative evaluation of arachidonic acid (AA)- and tetradecanoylphorbol acetate (TPA)-induced dermal inflammation. Inflammation. 1993;17(6):723-41.

상세보기
Oschman JL. Charge transfer in the living matrix. J Bodyw Mov Ther. 2009;13(3):215-28.

상세보기
Hancock RE, Haney EF, Gill EE. The immunology of host defence peptides: beyond antimicrobial activity. Nat Rev Immunol. 2016;16(5):321-34.

상세보기
Lo D, Feng L, Li L, Carson MJ, Crowley M, Pauza M, et al. Integrating innate and adaptive immunity in the whole animal. Immunol Rev. 1999;169:225-39.

상세보기
Nasef NA, Mehta S, Ferguson LR. Susceptibility to chronic inflammation: an update. Arch Toxicol. 2017;91(3):1131-41.

상세보기
Norris GH, Blesso CN. Dietary and Endogenous Sphingolipid Metabolism in Chronic Inflammation. Nutrients. 2017;9(11).

상세보기
Huang SS, Chiu CS, Chen HJ, Hou WC, Sheu MJ, Lin YC, et al. Antinociceptive activities and the mechanisms of anti-inflammation of asiatic Acid in mice. Evid Based Complement Alternat Med. 2011;2011:895857.

상세보기

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