[논문]질량분석기를 이용한 약령시장 내 유통 식물성 식품원료의 곰팡이독소 분석 및 위해성 평가

최은정; 고숙경; 조성애; 박영애; 정삼주; 홍성초; 조석주; 정지헌; 박주성

doi:10.22889/kjp.2022.53.3.162

[국내논문] 질량분석기를 이용한 약령시장 내 유통 식물성 식품원료의 곰팡이독소 분석 및 위해성 평가
Analysis of Multi-class Mycotoxins and Risk Assessment in Edible and Medicinal Plants by LC-MS/MS 원문보기

생약학회지, v.53 no.3, 2022년, pp.162 - 169

Abstract ▼ AI-Helper

This study investigated the mycotoxins (aflatoxin B1, B2, G1, G2, fumonisin B1, B2, ochratoxin A and zearalenone) contained in edible and medicinal plants in Seoul Yangnyeong market during 2020-2021. We analyzed contamination of mycotoxins using LC-MS/MS and evaluated risk assessment. The method was validated by assessing matrix effects, linearity, limit of detection (LOD), limit of quantification(LOQ) and recovery. Matrix-matched standard calibration was used for calibration curves showed good linearity (r2>0.999). The LOD, LOQ and recovery were 0.01-0.23 ㎍/kg, 0.04-0.71 ㎍/kg and 75.5-117.9% respectively. Mycotoxins were detected in 22 of 171 samples; aflatoxin B1 (6.66 ㎍/kg), fumonisin (7.54-64.68 ㎍/kg), ochratoxin A (4.21-10.56 ㎍/kg) and zearalenone (7.31-60.76 ㎍/kg). In the risk assessment, the MOE (Margine of Exposure) of aflatoxin B1 and ochratoxin A were in the range of 1.48×103-2.36×105. No items exceeded 100% in %TDI (Tolerable Daily Intake) of fumonisin (B1+B2) and zearalenone.

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표/그림 (8)

표 Table II. Analytical conditions of LC-MS/MS for mycotoxins
표 Table I. List of edible and medicinal plants for monitoring of mycotoxins
표 Table III. LC/MS-MS parameters for mycotoxins determination
그림 Fig. 1. LC-MS/MS chromatograms of mycotoxins in the standard solutions: AFB₁ and AFG₁ 10 μg/L; AFB₂ and AFG₂ 2.5 μg/L; FUMB₁, FUMB₂, OTA and ZEA 20 μg/L.
표 Table IV. Matrix-effect (ME) and Determination coefficient (r²)
표 Table V. LOD, LOQ and recovery of mycotoxins
표 Table VI. Incidence and level of mycotoxin contamination in edible and medicinal plants
표 Table VII. Risk assessment of mycotoxins

AI 본문요약
AI-Helper

성능/효과

본 연구의 결과에서 식·약공용농산물의 과실류, 근경류 및 종자류에서 8종의 곰팡이독소의 오염도는 우려할 수준이 아닌 것으로 나타났다
약령시 내 식·약공용농산물의 위해성 평가는 위해 우려가 낮은 수준으로 평가되었다

후속연구

그러나 곰팡이독소 오염도의 불검출율에 따라 위해 평가시 과대평가 될 가능성이 있으며, 식·약공용농산물은 단일, 혼합으로 가열 추출 및 환 등으로 국민들의 섭취 방법이 다양하고, 이에 따른 곰팡이독소 이행률에 의한 불확실성이 존재할 가능성이 있어 다양한 노출 환경에 따른 평가가 필요하다.
그러나 곰팡이독소 생성에 영향을 줄수 있는 국내 및 수입국의 기후 변화, 식·약공용농산물의 용도 확대에 따른 독소 노출 가능성이 증가할 수 있다. 따라서 국내 및 수입국의 신뢰성과 대표성이 확보된 다양한 품목과 시료 수로 지속적인 곰팡이독소의 모니터링이 필요하다고 생각된다.

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