[논문]Biological Control Potential of Penicillium brasilianum against Fire Blight Disease

Kim, Yeong Seok; Ngo, Men Thi; Kim, Bomin; Han, Jae Woo; Song, Jaekyeong; Park, Myung Soo; Choi, Gyung Ja; Kim, Hun

doi:10.5423/ppj.oa.06.2022.0076

Biological Control Potential of Penicillium brasilianum against Fire Blight Disease 원문보기

The plant pathology journal, v.38 no.5, 2022년, pp.461 - 471

Kim, Yeong Seok (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) , Ngo, Men Thi (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) , Kim, Bomin (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) , Han, Jae Woo (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) , Song, Jaekyeong (Agricultural Microbiology Division, National Institute of Agricultural Sciences, Rural Development Administration) , Park, Myung Soo (Department of School of Biological Sciences, Seoul National University) , Choi, Gyung Ja (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) , Kim, Hun (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)

Abstract ▼ AI-Helper

Erwinia amylovora is a causative pathogen of fire blight disease, affecting apple, pear, and other rosaceous plants. Currently, management of fire blight relies on cultural and chemical practices, whereas it has been known that few biological resources exhibit disease control efficacy against the fire blight. In the current study, we found that an SFC20201208-M01 fungal isolate exhibits antibacterial activity against E. amylovora TS3128, and the isolate was identified as a Penicillium brasilianum based on the 𝛽-tubulin (BenA) gene sequence. To identify active compounds from the P. brasilianum culture, the culture filtrate was partitioned with ethyl acetate and n-butanol sequentially. From the ethyl acetate layer, we identified two new compounds (compounds 3-4) and two known compounds (compounds 1-2) based on spectroscopic analyses and comparison with literature data. Of these active compounds, penicillic acid (1) exhibited promising antibacterial activity against E. amylovora TS3128 with a minimal inhibitory concentration value of 25 ㎍/ml. When culture filtrate and penicillic acid (125 ㎍/ml) were applied onto Chinese pearleaf crab apple seedlings prior to inoculation of E. amylovora TS3128, the development of fire blight disease was effectively suppressed in the treated plants. Our results provide new insight into the biocontrol potential of P. brasilianum SFC20201208-M01 with an active ingredient to control fire blight.

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표/그림 (8)

그림 Fig. 1. Phylogenetic analysis of an isolate SFC20201208-M01 based on the sequence of β-tubulin (BenA) gene.
그림 Fig. 2. Antibacterial activity of an SFC20201208-M01 isolate against Erwinia amylovora TS3128. (A) The cultural phenotype of Penicillium brasilianum SFC20201208-M01 on various media. The upper layer showed cultures grown solid plate, and the lower layer showed cultures grown liquid medium. (B) Antibacterial activity of culture filtrates derived from P. brasilianum SFC20201208-M01 grown in various media against E. amylovora TS3128. SDB, Sabouraud’s dextrose broth; MEB, malt extract broth; PDB, potato dextrose broth; CDB, Czapek-Dox broth medium; NC, normal control. (C) Minimal inhibitory concentration (MIC) and IC50 values of culture filtrate of P. brasilianum SFC20201208-M01 grown on PDB medium at 25°C for 14 days.
그림 Fig. 3. Isolation and identification of active compounds from Penicillium brasilianum SFC20201208-M01. (A) Isolation scheme of active compounds from P. brasilianum SFC20201208-M01 culture filtrate. (B) High-performance liquid chromatography chromatograms of organic solvent extracts of P. brasilianum SFC20201208-M01 culture. (C) Chemical structures of active compounds identified from P. brasilianum SFC20201208-M01. 1, penicillic acid; 2, 5,6-dihydro-6-hydroxypenicillic acid; 3, cis-brasilianone; 4, trans-brasilianone.
그림 Fig. 4. The key 2D-NMR correlations of compounds 3 and 4. NMR, nuclear magnetic resonance.
표 Table 1. ¹H and ¹³C NMR data of compounds 1 and 2 (400 MHz in methanol-d₄)
표 Table 2. ¹H and ¹³C NMR data of compounds 3 and 4 (400 MHz in methanol-d4)
그림 Fig. 5. In vivo antibacterial activity of culture filtrate of Penicillium brasilianum SFC20201208-M01 and penicillic acid. Representatives of Chinese pearleaf crab apple seedlings treated with culture filtrate (A) and penicillic acid (B). Treatments of streptomycin (100 μg/ml) and 1% dimethyl sulfoxide were used as positive and negative controls, respectively. Plants were inoculated with a cell suspension (4 × 108 cfu/ml) of Erwinia amylovora TS3128 at 24 h after the treatment. Photos were taken at 9 dpi. (C) Effect of penicillic acid on bacterial growth on apple leaves. Photos were taken at 48 hpi. The values represent the mean ± standard deviation of two runs with three replicates. Asterisks (*) indicate significant differences at P < 0.05. NC, non-treatment control; DSI, disease severity index; PA, penicillic acid; PT, phytotoxicity; ST, streptomycin.
표 Table 3. In vitro antibacterial activity of compounds 1-4 against Erwinia amylovora TS3128

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