[논문]반응표면법에 의한 Lactiplantibacillus plantarumK79를 이용한 ACE(Angiotensin Converting Enzyme) 억제활성 향상을 위한 탈지유 발효조건 최적화

박유경; 홍상필; 임상동

doi:10.22424/jdsb.2022.40.3.93

반응표면법에 의한 Lactiplantibacillus plantarumK79를 이용한 ACE(Angiotensin Converting Enzyme) 억제활성 향상을 위한 탈지유 발효조건 최적화
Optimization of Skim Milk Fermentation Conditions by Response Surface Methodology to Improve ACE Inhibitory Activity Using Lactiplantibacillus plantarum K79 원문보기

Journal of dairy science and biotechnology, v.40 no.3, 2022년, pp.93 - 102

박유경 (한국식품연구원) , 홍상필 (한국식품연구원) , 임상동 (한국식품연구원)

초록
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본 연구는 L. plantarum K79를 이용하여 ACE 억제활성 향상을 위한 최적의 발효조건을 RSM을 이용하여 예측하고자 하였다. 4개의 독립변수[탈지유(포도당 1% 첨가) 농도(6%-14%), 배양 온도(32℃-42℃), 배양 시간(8-24시간), 스타터 첨가량(0.02%-0.2%)] 5단계 중심 합성 설계 및 반응 표면 분석법을 사용하여 최적의 발효 조건을 결정하는 데 사용하였다. 종속변수는 ACE 억제 활성과 pH였다(이때 ACE 억제율은 조건별 발효원액에서 100배 희석하여 나타낸 값이다). 결정 계수(R²)는 ACE 억제 활성, pH에 대해 각각 0.791, 0.905이었다. 최대 ACE 억제 활성은 10% 탈지유(포도당 1% 첨가) 농도, 37℃ 배양 온도, 17.8 h 배양 시간 및 0.2% 스타터 첨가량 조건에서 90%이었다. RSM에 기초하여 예측된 최적 발효 ACE 조건은 탈지유(포도당 1% 첨가) 농도 13.49%, 스타터 0.0578%, 배양온도 33.4℃에서 21.5시간 동안 배양할 때 ACE 억제 활성 및 pH의 예측 값은 86.69% 및 pH 4.6이었으며 실제 값은 각각 85.5%와 pH 4.58이었다.

Abstract ▼ AI-Helper

This study was conducted using response surface methodology (RSM) to elucidate fermentation conditions that will optimize ACE inhibitory activity using Lactiplantibacillus plantarum K79. Four independent variables [skim milk (with 1% added glucose) concentration (6%-14%), incubation temperature (32℃-42℃), incubation time (8-24 h), and amount of added starter (0.02%-0.2%)] were evaluated using five-level central composite design and response surface methodology to determine the optimum fermentation condition. The dependent variables were angiotensin converting enzyme (ACE) inhibitory activity (the value obtained from 102 diluted supernatant), and pH. The respective coefficients of determinations (R2) were 0.791 and 0.905 for ACE inhibitory activity and pH. The maximum ACE inhibitory activity was 90% under the following conditions: 10% skim milk (with 1% added glucose) concentration, 37℃ incubation temperature, 17.8 h incubation time, and 0.2% added starter. Based on the RSM, using predicted best ACE conditions for fermentation of 13.49% skim milk (with 1% added glucose) with 0.0578% starter at 33.4℃ for 21.5 h, the predicted ACE inhibitory activity and pH values were 86.69% and 4.6, respectively. Actual ACE inhibitory activity and pH values were 85.5% and 4.58, respectively

주제어

표/그림 (8)

표 Table 1. Independent values, their coded and actual values for optimization condition of fermented milk by Lactiplantibacillus plantarum K79
표 Table 2. Central composite design and responses of dependent variables for fermented milk by Lactiplantibacillus plantarum K79 to independent variables
표 Table 3. Estimated effects and coefficients for ACE inhibitory activity and pH (coded units) about fermented milk by Lactiplantibacillus plantarum K79
표 Table 4. Response surface model for making condition
표 Table 5. Analysis of variance for ACE inhibitory activity, pH (coded units) about fermented milk by Lactiplantibacillus plantarum K79
표 Table 6. Optimal conditions of ACE inhibitory activity and pH
그림 Fig. 1. Response surface plots for the effect of independent variables on dependent (ACE inhibitory activity and pH) in Lactobacillus plantarum K79. X₁, skim milk conc. (%); X₂, temperature (℃); X₃, incubation time (h); X₄, starter conc. (%); Y₁, ACE inhibitory activity (%); Y₂, pH; ACE, angiotensin converting enzyme.
표 Table 7. Predicted results of verification under optimized conditions

AI 본문요약
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문제 정의

따라서 본 연구에서는 탈지유 농도, 스타터 첨가량, 배양온도, 배양시간을 이용하여 ACE 억제활성을 극대화하기 위하여 반응표면분석법을 통해 발효조건을 확립하고자 하였다.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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