[논문]양자점 기반 다중 바이오마커 검출법의 연구동향

김예린; 최유림; 김봉근; 나현빈

doi:10.14478/ace.2022.1093

양자점 기반 다중 바이오마커 검출법의 연구동향
Recent Progress in Multiplexed Detection of Biomarkers Based on Quantum Dots 원문보기

공업화학 = Applied chemistry for engineering, v.33 no.5, 2022년, pp.451 - 458

김예린 (명지대학교 화학공학과) , 최유림 (명지대학교 화학공학과) , 김봉근 (명지대학교 화학공학과) , 나현빈 (명지대학교 화학공학과)

초록
AI-Helper

반도체 양자점은 우수한 형광 특성을 가진 광학 탐침자로 생명-의학 영상화 기술 및 바이오센싱 분야에서 광범위하게 활용되고 있다. 양자점은 넓은 광흡수 에너지띠, 좁은 형광 에너지띠와 같은 광학 특성을 가지므로 서로 다른 형광 파장을 지닌 양자점을 조합해 다종의 신호를 생성할 수 있도록 구성하면 복수의 바이오마커를 동시에 검출할 수 있다. 본 총설에서는 이와 같은 다중 검출 분석법에서의 양자점 및 이에 기반한 양자점 나노비드가 가지는 장점과 활용 사례를 기술하고 다중 형광 바이오마커 검출법의 최근 개발 동향 및 개선사항을 요약 정리하였다. 특히 양자점을 활용한 형광-결합 면역흡착 분석법, 양자점 나노비드를 이용한 면역크로마토그래피 분석법 등 면역 분석법에서의 신호 전환 소재 디자인을 중심으로 최근의 연구 결과를 검토하였다. 정확성과 민감도가 우수한 다중 바이오마커 검출 기술이 확보된 데이터를 처리하고 해석하는 인공지능 알고리즘과 결합될 경우 질병의 조기 진단을 포함한 다양한 분야에 활용가능한 새로운 검출 플랫폼의 개발로 이어질 것으로 기대된다.

Abstract ▼ AI-Helper

Semiconductor quantum dots (QDs) are optical probes with excellent fluorescence properties. Therefore, they have been applied to various bio-medical imaging techniques and biosensors. Due to the unique optical characteristics of wide absorption and narrow fluorescence energy bands, multiple types of signals can be generated by the combination of fluorescence wavelengths from different QDs, which enables the simultaneous detection of more than two biomarkers. In this review, the advantages and applications of QDs and QD nanobeads (QBs) in multiple biomarker assays were described, and new developments or improvements in multiplexed biomarker detection techniques were summarized. In particular, recent reports were summarized, focusing on the design strategies in immunoassay construction and signal transducing materials for fluorescence-linked immunosorbent assays using QDs and immunochromatographic assays using QBs. New detection platforms will be developed for early diagnosis of diseases and other fields if multiplexed detection technologies of excellent accuracy and sensitivity are combined with artificial intelligence algorithms.

주제어

표/그림 (3)

그림 Figure 1. Schematic illustration of preparation for the antibody-conjugated QDs and dQDs-FLISA procedure.
그림 Figure 2. Schematic representations for the synthesis of tricolor QBs via using emulsification evaporation method (a) and dual color QBs by coatingof QDs onto SiO₂ nanoparticles (b). (c) Schematic representation of the multiplexed ICA strip.
그림 Figure 3. (A) Schematic illustration of the assembly of QD-Cy3-Texas Red-Cy5 tetrahedron DNA nanostructure for multiple enzymes assay. (B) Biotin-Cy3-Texas Red-Cy5 tetrahedron DNA contains the endonuclease incision sites for PvuII, EcoRV, and HaeIII. (C) Schematic illustration of FRET between QD and dyes [32]. (Reprinted with permission from Ref. [32]).

참고문헌 (34)

B. Purohit, P. R. Vernekar, N. P. Shetti, and P. Chandra, Biosensor nanoengineering: Design, operation, and implementation for biomolecular analysis, Sens. Int., 1, 100040 (2020).

상세보기
J. Lee, T. Kim, B.-G. Kim, and H. B. Na, Recent progress in inorganic nanoparticles with enzyme-mimetic activities and their applications to diagnosis and therapy, Appl. Chem. Eng., 31, 352-359 (2020).

원문보기 상세보기
M. Harun-Ur-Rashid, T. Foyez, I. Jahan, K. Pal, and A. B. Imran, Rapid diagnosis of COVID-19 via nano-biosensor-implemented biomedical utilization: a systematic review, RSC Adv., 12, 9445-9465 (2022).

상세보기
V. Naresh, N. Lee, Review on biosensors and recent development of nanostructured materials-enabled biosensors, Sensors, 21, 1109 (2021).
Y. I. Park, Inorganic Nanoparticles for Near-infrared-II Fluorescence Imaging, Appl. Chem. Eng., 33, 17-27 (2022).
S. M. Mousavi, S. A. Hashemi, M. Y. Kalashgrani, N. Omidifar, C. W. Lai, N. V. Rao, A. Gholami, and W.-H. Chiang, The pivotal role of quantum dots-based biomarkers integrated with ultra-sensitive probes for multiplex detection of human viral infections, Pharmaceuticals, 15, 880 (2022).

상세보기
R. C. Castro, M. L. M. F. S. Saraiva, J. L. M. Santos, and D. S. M. Ribeiro, Multiplexed detection using quantum dots as photoluminescent sensing elements or optical labels, Coord. Chem. Rev., 448, 214181 (2021).

상세보기
G. Jarockyte, V. Karabanovas, R. Rotomskis, and A. Mobasheri, Multiplexed Nanobiosensors: Current Trends in Early Diagnostics, Sensors, 20, 6890 (2020).

상세보기
C. Li, Y. Yang, D. Wu, T. Li, Y. Yin, and G. Li, Improvement of enzyme-linked immunosorbent assay for the multicolor detection of biomarkers, Chem. Sci., 7, 3011-3016 (2016).

상세보기
H. Y. Shin, B.-G. Kim, S. Cho, J. Lee, H. B. Na, and M. I. Kim, Visual determination of hydrogen peroxide and glucose by exploiting the peroxidase-like activity of magnetic nanoparticles function-alized with a poly(ethylene glycol) derivative, Microchim. Acta, 184, 2115-2122 (2017).

상세보기
D. Quesada-Gonzalez and A. Merkoci, Nanoparticle-based lateral flow biosensors, Biosens. Bioelectron., 73, 47-63 (2015).

상세보기
F. A. Esteve-Turrillas and A. Abad-Fuentes, Applications of quantum dots as probes in immunosensing of small-sized analytes, Biosens. Bioelectron., 41, 12-29 (2013).

상세보기
Y. Park, S. Jeong, and S. Kim, Medically translatable quantum dots for biosensing and imaging, J. Photochem. Photobiol. C: Photochem. Rev., 30, 51-70 (2017).

상세보기
W. Wang and H. Mattoussi, Engineering the bio-nano interface using a multifunctional coordinating polymer coating, Acc. Chem. Res., 53, 1124-1138 (2020).

상세보기
Y. Lv, F. Wang, N. Li, R. Wu, J. Li, H. Shen, L. S. Li, and F. Guo, Development of dual quantum dots-based fluorescence-linked immunosorbent assay for simultaneous detection on inflammation biomarkers, Sens. Actuators B Chem., 301, 127118 (2019).

상세보기
J. Zhou, Z. Liu, Q. Yang, W. Qian, Y. Chen, Y. Qi, and A. Wang, Multiple fluorescence immunoassay for the simultaneous detection of Zearalenone and Ochratoxin A, Anal. Biochem., 628, 114288 (2021).

상세보기
Z. Su, W. Dou, X. Liu, J. Ping, D. Li, Y. Ying, and L. Xie, Nano-labeled materials as detection tags for signal amplification in immunochromatographic assay, Trends Analyt. Chem., 154, 116673 (2022).
R. Li, Y. Wen, L. Yang, A. Liu, F. Wang, and P. He, Dual quantum dot nanobeads-based fluorescence-linked immunosorbent assay for simultaneous detection of aflatoxin B1 and zearalenone in feed-stuffs, Food Chem., 366, 130527 (2022).

상세보기
L. Ding, X. Chen, L. He, F. Yu, S. Yu, J. Wang, Y. Tian, Y. Wang, Y. Wu, L.-E. Liu, and L. Qu, Fluorometric immunoassay for the simultaneous determination of the tumor markers carcinoembryonic antigen and cytokeratin 19 fragment using two kinds of CdSe/ZnS quantum dot nanobeads and magnetic beads, Microchim. Acta, 187, 171 (2020).

상세보기
W. Wu, X. Liu, M. Shen, L. Shen, X. Ke, D. Cui, and W. Li, Multicolor quantum dot nanobeads based fluorescence-linked immunosorbent assay for highly sensitive multiplexed detection, Sens. Actuators B. Chem., 338, 129827 (2021).

상세보기
Y. Wu, J. Sun, X. Huang, W. Lai, and Y. Xiong, Ensuring food safety using fluorescent nanoparticles-based immunochromatographic test strips, Trends Food Sci. Technol., 118, 658-678 (2021).

상세보기
H. Duan, Y. Li, Y. Shao, X. Huang, and Y. Xiong, Multicolor quantum dot nanobeads for simultaneous multiplex immunochromatographic detection of mycotoxins in maize, Sens. Actuators B Chem., 291, 411-417 (2019).

상세보기
H. Cao, S. Cao, Y. Han, W. Zhang, Z. Wei, T. Ye, M. Yuan, J. Yu, X. Wu, L. Hao, F. Yin, D. Feng, and F. Xu, Synthesis of quantum dot encoded multicolour nanobeads for the ultrasensitive and multiplex immunochromatographic detection of neonicotinoid insecticides, Sens. Actuators B Chem., 361, 131671 (2022).

상세보기
Y. Cao, Z. Chen, X. Li, Z. Li, G. Lin, T. Liu, and Y. Wu, Dual-color quantum dot-loaded nanoparticles based lateral flow biosensor for the simultaneous detection of gastric cancer markers in a single test line, Anal. Chim. Acta, 1218, 339998 (2022).

상세보기
R. Li, C. Meng, Y. Wen, W. Fu, and P. He, Fluorometric lateral flow immunoassay for simultaneous determination of three mycotoxins (aflatoxin B1, zearalenone and deoxynivalenol) using quantum dot microbeads, Microchim. Acta, 186, 748 (2019).

상세보기
X. Yang, X. Liu, B. Gu, H. Liu, R. Xiao, C. Wang, and S. Wang, Quantitative and simultaneous detection of two inflammation biomarkers via a fluorescent lateral flow immunoassay using dual-color SiO 2 @QD nanotags, Microchim. Acta, 187, 570 (2020).

상세보기
J. Xiong, L. Qin, H. Zhang, S. Zhang, S. He, Y. Xu, L. Zhang, Z. Wang, and H. Jiang, Sensitive and simultaneous detection of ractopamine and salbutamol using multiplex lateral flow immunoassay based on polyethyleneimine-mediated SiO 2 @QDs nanocomposites: Comparison and application, Microchem. J., 181, 107730 (2022).
X. Liu, X. Ren, L. Chen, J. Zou, T. Li, L. Tan, C. Fu, Q. Wu, C. Li, J. Wang, Z. Huang, and X. Meng, Fluorescent hollow ZrO 2 @CdTe nanoparticles-based lateral flow assay for simultaneous detection of C-reactive protein and troponin T, Microchim. Acta, 188, 209 (2021).

상세보기
L. Ao, T. Liao, L. Huang, S. Lin, K. Xu, J. Ma, S. Qiu, X. Wang, and Q. Zhang, Sensitive and simultaneous detection of multi-index lung cancer biomarkers by an NIR-II fluorescence lateral-flow immunoassay platform, Chem. Eng. J., 436, 135204 (2022).
A. Kaur and S. Dhakal, Recent applications of FRET-based multiplexed techniques, Trends Analyt. Chem., 123, 115777 (2020).
X. Jiao, Y. Zhou, D. Zhao, D. Pang, C. Wang, H. Du, Y. Wen, and X. Zhang, An indirect ELISA-inspired dual-channel fluo-rescent immunoassay based on MPA-capped CdTe/ZnS QDs, Anal. Bioanal. Chem., 411, 5437-5444 (2019).

상세보기
J. Hu, M.-H. Liu, and C.-Y. Zhang, Construction of tetrahedral dna-quantum dot nanostructure with the integration of multistep forster resonance energy transfer for multiplex enzymes assay, ACS Nano, 13, 7191-7201 (2019).

상세보기
S. Bhuckory, K. D. Wegner, X. Qiu, Y.-T. Wu, and T. L. Jennings, A. Incamps, and N. Hildebrandt, Triplexed CEA-NSE-PSA immunoassay using time-gated terbium-to-quantum dot FRET, Molecules, 25, 3679 (2020).

상세보기
F. Cui, Y. Yue, Y. Zhang, Z. Zhang, and H. S. Zhou, Advancing biosensors with machine learning, ACS Sens., 5, 3346-3364 (2020).

상세보기

저자의 다른 논문 :

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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