[논문]작약(Paeonia lactiflora Pall.) 뿌리절편 유래 캘러스 배양으로부터 부정근발생을 위한 최적 배양조건

이영진; 최명석; 최필선

doi:10.5010/jpb.2022.49.3.207

작약(Paeonia lactiflora Pall.) 뿌리절편 유래 캘러스 배양으로부터 부정근발생을 위한 최적 배양조건
Optimal conditions for adventitious root organogenesis from peony root explant callus cultures 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.49 no.3, 2022년, pp.207 - 212

이영진 (남부대학교 응급구조학과) , 최명석 (경상대학교 농업생명과학대학 환경산림과학부) , 최필선 (남부대학교 응급구조학과)

초록
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작약 뿌리 절편으로부터 유도된 캘러스 클론으로부터 부정근 발생에 대한 최적의 배양조건을 조사하기 위하여 뿌리 절편으로부터 캘러스 유도를 위해서 먼저 0.5, 1.0, 2.0, 3.0, 4.0 mg/L 농도의 오옥신(IAA, NAA, IBA, 2,4-D)과 0, 0.5, 1.0, 2.0 mg/L 농도의 싸이토카닌(kinetin, zeatin, BAP)를 조합한 MS 배지에서 배양하였다. 캘러스 클론으로부터 부정근 유도와 길이 생장은 0.1, 0.5, 1.0 mg/L 농도의 오옥신(IAA, NAA, IBA) 또는 싸이토카닌(kinetin, zeatin, BAP)을 단독으로 첨가한 배지에서 암 조건으로 그리고 0.1, 0.5, 1.0 mg/L 농도의 IBA와 zeatin을 각각 조합 첨가한 배지에서 명/암 조건으로 6주 동안 배양하였다. 캘러스 형성은 다른 조합 처리보다 1.0 mg/L 농도의 NAA와 zeatin을 조합 첨가한 배지에서 가장 효과적이었으며, 캘러스 클론으로부터 부정근 발생 수와 부정근의 길이 생장은 IBA 단독처리의 경우 각각 6.66개와 4.82 cm, zeatin 단독 처리의 경우 2.32개와 0.92 cm로 다른 호르몬에 비해 우수하였다. 특히 0.1 mg/L IBA와 0.5 mg/L zeatin을 조합 첨가한 배지에서 광 조건으로 배양할 경우 가장 많은 부정근이(14.06) 형성되었으며, 동일배지에서 암 조건으로 배양할 경우 부정근의 길이가 가장 긴 5.45 cm로 측정되어 가장 효과적인 농도와 조합임을 알 수 있었다. 이러한 작약의 기내 배양에 대한 최적의 배양 조건은 기내배양을 통해 작약 부정근의 대량 생산을 위한 배지로 사용할 수 있음을 보여 주었다.

Abstract ▼ AI-Helper

The optimal culture conditions for root organogenesis from the callus of peonies (Paeonia lactiflora Pall.) were investigated. Root explants with vascular bundles were cultured in Murashige and Skoog (MS) medium combined with 0.5-4.0 mg/L auxins (indole acetic acid [IAA], naphthalene acetic acid [NAA], indolebutyric acid [IBA], and 2,4-dichlorophenoxyacetic acid [2,4-D]) and 0.0-2.0 mg/L cytokinins (kinetin, zeatin, and benzylaminopurine [BAP]) to induce callus formation. The callus was then cultured in MS medium combined with three concentrations (0.1, 0.5, and 1.0 mg/L) of IAA, NAA, IBA, kinetin, zeatin, and BAP in the dark for 6 weeks. Based on the results, the effects of dark and light conditions on the callus cultured in MS medium with combinations of 0.1-1.0 mg/L IBA and zeatin for 6 weeks were studied. Callus formation was most effective (>+++) in the medium with a combination of 1.0 mg/L NAA and 1.0 mg/L zeatin. A high number of long adventitious roots were observed in the mediums with 0.1 mg/L IBA (6.66 and 4.82 cm) and 0.5 mg/L zeatin (2.32 and 0.72 cm) among auxins and cytokinins, respectively. The highest number (14.06) of adventitious roots were formed from the callus cultured in light in the MS medium combined with 0.1 mg/L IBA and 0.5 mg/L zeatin. This same medium induced the formation of the longest adventitious root (5.45 cm) in the dark. Thus, optimization of in vitro culture conditions may be possible for the mass propagation of adventitious roots in peonies.

주제어

표/그림 (4)

그림 Fig. 1 Adventitious root formation on callus derived from root explant cultures of Paeonia lactiflora Pall. Light yellowish compact callus from root explant cultured in MS medium with 1.0 mg/L NAA and 1.0 mg/L zeatin (A) and adventitious roots formed from callus clone cultures in MS medium with 0.1 mg/L IAA (B), 0.1 mg/L IBA (C), 0.5 mg/L zeatin (D), 0.1 mg/L kinetin (E), and a combination of 0.1 mg/L IBA and 0.5 mg/L zeatin (F). MS: Murashige and Skoog, IAA: indole acetic acid, NAA, naphthalene acetic acid, IBA: indolebutyric acid, 2,4-D: 2,4-dichlorophenoxyacetic acid, BAP: benzylaminopurine
표 Table 1 Effects of plant growth regulators on callus formation from root explant of Paeonia lactiflora Pall. cultured in Murashige and Skoog medium containing auxins and cytokinins in the dark for 6 weeks
표 Table 2 Optimal amount of auxin and cytokinin required for the greatest number and length of adventitious roots formed from callus clone cultures of Paeonia lactiflora Pall. in Murashige and Skoog medium containing various concentrations of plant growth regulators grown in dark condition for 6 weeks
표 Table 3 Effects of dark and light conditions after 6 weeks on the number and length of adventitious roots formed from callus clone cultures of Paeonia lactiflora Pall. in MS medium containing IBA and zeatin

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