[논문]순무가루를 첨가한 순무김치의 항산화 활성 및 대장암세포(HT-29) 항암효과

권국원; 강순아

doi:10.9799/ksfan.2022.35.5.359

순무가루를 첨가한 순무김치의 항산화 활성 및 대장암세포(HT-29) 항암효과
Antioxidant Activity and Anticancer Effects of Turnip Kimchi with Turnip Powder on Colorectal Cancer Cells (HT-29) 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.35 no.5, 2022년, pp.359 - 368

권국원 (호서대학교 보건산업연구소) , 강순아 (호서대학교 벤처대학원 융합공학과)

Abstract ▼ AI-Helper

In this study, the quality characteristics of kimchi, such as its salinity, pH, and acidity, were measured and compared, and the HT-29 human colon cancer cells were used to show the anticancer effects of kimchi. The kimchi samples used herein included standard kimchi (SK), turnip kimchi (TK), and turnip-powder-added kimchi (TPK). The measured pH and acidity of TK and TPK showed no significant differences with those of SK. Compared to SK and TK, TPK had higher DPPH scavenging activity and higher total flavonoid content, confirming its antioxidant activity. The cancer cell growth inhibition rates of TK and TPK were significantly higher than that of SK. In HT-29 cells treated with TPK, the mRNA expression of Bcl-xL, an anti-apoptosis-related gene, was lower, and the mRNA expressions of the apoptosis-related genes Bax, Bad, and caspase-9 were higher. TPK showed significantly higher levels of mRNA expressions for the cell-cycle-related genes p53 and p21 than the other samples, in addition to suppression effects on cancer cell proliferation. Compared to SK, TK and TPK suppressed the growth of colon cancer cells and showed higher anticancer effects. Therefore, it is shown that kimchi with added turnip powder had high anticancer effects.

주제어

표/그림 (9)

표 Table 1. Ingredients of kimchi
그림 Fig. 1. Change of salinity in various kinds of kimchi fermented at 5℃. SK: standard kimchi. TK: turnip kimchi. TPK: turnip powder kimchi. ^a-cMeans with the different letters at the same storage period are significantly different (p<0.05) by Duncan's multiple range tests. ^NSnot significantly different.
표 Table 2. The primers used in real-time PCR
그림 Fig. 2. Change of pH (a) and acidity (b) in various kinds of kimchi during fermentation at 5℃. SK: standard kimchi. TK: turnip kimchi. TPK: turnip powder kimchi. ^a-cMeans with the different letters at the same storage period are significantly different (p<0.05) by Duncan's multiple range tests. ^NSnot significantly different.
그림 Fig. 3. DPPH radical scavenging effect of various kinds of kimchi. SK: standard kimchi. TK: turnip kimchi. TPK: turnip powder kimchi. ^a,bMeans with the different letters at the same concentration are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 4. Total Flavonoid contents of various kinds of kimchi. SK: standard kimchi. TK: turnip kimchi. TPK: turnip powder kimchi. ^a,bMeans with the different letters at the same concentration are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 5. MTT assay of kimchi in the HT-29 human colon cancer cells. SK: standard kimchi. TK: turnip kimchi. TPK: turnip powder kimchi. ^a,bMeans with the different letters at the same concentration are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 6. mRNA expression levels of apoptosis related genes (Bcl-xL, Bax, Bad, caspase-9) in the HT-29 human colon cancer cells. Con: control group, SK: standard kimchi, TK: turnip kimchi, TPK: turnip powder kimchi. ^a-cMeans with the different letters at the same concentration are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 7. mRNA expression levels of cell cycle arrest related genes (p21, p53) in the HT-29 human colon cancer cells. Con: control group, SK: standard kimchi, TK: turnip kimchi, TPK: turnip powder kimchi. ^a-cMeans with the different letters at the same concentration are significantly different (p<0.05) by Duncan's multiple range tests.

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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