[논문]UVB로 산화적 손상을 유도한 피부섬유아세포에 Saponaria 추출물의 보호효과

김보애

doi:10.12925/jkocs.2022.39.5.644

UVB로 산화적 손상을 유도한 피부섬유아세포에 Saponaria 추출물의 보호효과
Protective Effect of Saponaria Extract Against UVB-Damage in Skin Fibroblasts 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.39 no.5, 2022년, pp.644 - 651

초록
AI-Helper

피부는 인체를 구성하는 가장 큰 장기로 생체 내부를 보호한다. 자외선은 피부에 광노화와 산화적 손상을 비롯한 다양한 염증반응을 일으킨다. 본 연구의 목적은 섬유아세포에서 UVB를 조사하여 Saponaria 추출물의 보호 효과를 조사하는 것이다. 본 연구에서는 UVB에 의한 세포독성과 산화적 세포사멸, NO 및 PGE₂ 생성에 대한 보호활성을 나타내는 Saponaria의 유효성을 평가하였다. HS68 세포를 UVB(120mJ/cm²)에 조사하고 100, 200, 400 ㎍/mL의 다양한 농도로 Saponaria 추출물로 24시간 동안 처리하였으며, 자외선 B에 의해 생성된 세포 내 활성 산소 종(ROS)은 DCF-DA 염색 후 분광 형광계를 사용하여 검출하였다. 또한 지질 과산화는 배양 배지로 분비되는 8-이소프로스탄의 수준을 측정하여 분석하였다. 그 결과 Saponaria 추출물이 UVB에 의한 세포독성을 효과적으로 억제하였다. 산화적 세포 손상은 UVB로 유도된 HS68 섬유아세포에서 PGE₂를 매개하였고, 이는 사포나리아 추출물 처리에 의하여 유의하게 억제되었다. 또한, 이들 추출물의 보호 효과는 농도 의존적으로 세포내 ROS 생성 및 지질 과산화 억제에 의해 매개되는 것으로 평가되었다. 이러한 결과는 Saponaria 추출물이 자외선 B에 의한 산화적 스트레스로 매개한 피부 손상을 억제하여 세포 보호효과를 나타내므로 항노화 기능성 소재로 활용될 수 있을 것으로 사료된다.

Abstract ▼ AI-Helper

The skin is the largest organ of the human body and protects the inside of the body. Ultraviolet rays cause various inflammatory reactions in the skin, including photoaging and oxidative damage. The purpose of this study is to investigate the protective effect of Saponaria extract by irradiating UVB on fibroblasts. In this study, the effectiveness of Saponaria showing protective activity against UVB-induced cytotoxicity, oxidative cell death, and NO and PGE2 production was evaluated. HS68 cells were irradiated with UVB(120 mJ/cm2) and treated with Saponaria extract at various concentrations of 100, 200, and 400 ㎍/mL for 24 hours. Intracellular reactive oxygen species (ROS) generated by ultraviolet B were detected using a spectrofluorometer after DCF-DA staining. Lipid peroxidation was also analyzed by measuring the level of 8-isoprostane secreted into the culture medium. As a result, treatment with Saponaria extract effectively inhibited UVB-induced cytotoxicity. Oxidative cell damage was mediated by PGE2 in UVB-induced HS68 fibroblasts, which was significantly inhibited by Saponaria extract treatment. In addition, it was evaluated that the protective effect of these extracts was mediated by the inhibition of intracellular ROS production and lipid peroxidation in a concentration-dependent manner. These results suggest that Saponaria extract can be used as an anti-aging functional material because it inhibits skin damage mediated by oxidative stress caused by UVB and exhibits a cellular protective effect.

주제어

표/그림 (4)

그림 Fig. 1. Protective effect of Sponaria extract on the UVB-induced cytotoxicity. HS68 cells were exposed to UVB (120 mJ/cm²) and treated with various concentrations(100, 200, 400 μ g/mL) of Sponaria extract for additional 24 h. The data is expressed as percentage cell viability and represent the means. Data shown are from a representative experiment repeated three times with similar results. The values are the mean±standard error of mean. **p < 0.001 compared the control group.
그림 Fig. 2. The Saponaria extract suppressed UVB-induced NO and PGE₂ production. HS68 cells were exposed with UVB alone or with various concentrations of extracts. The data is expressed as percentage cell viability and represent the means. Data shown are from a representative experiment repeated three times with similar results. The values are the mean±standard error of mean. *p < 0.05 compared the UVB-alone group.
그림 Fig. 3. Cellular antioxidant activity of Saponaria extract in UVB-induced oxidative stress in HS68 cells. Intracellular reactive oxygen species (ROS) levels generated by UV radiation were detected using a spectrofluorometer after DCF-DA staining. The data is expressed as percentage cell viability and represent the means. Data shown is from a representative experiment repeated three times with similar results. The values are the mean±standard error of mean. **p < 0.001 compared the UVB-alone control group.
그림 Fig. 4. Modulation of UVB-induced lipid peroxidation by Saponaria extract. HS68 cells were irradiated with UVB and then treated with Saponaria extract for 12 h. The lipid peroxidation was assayed by measuring the levels of 8-isoprostane secreted into the culture medium. The data are expressed as percentage cell viability and represent the means. Data shown is from a representative experiment repeated three times with similar results. The values are the mean±standard error of mean. *p < 0.05 compared the UVB-alone group.

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