[논문]Passiflora caerulea 추출물의 화장품 소재 활용 가능성

이재남

doi:10.12925/jkocs.2022.39.5.710

Passiflora caerulea 추출물의 화장품 소재 활용 가능성
The Possibility of Passiflora Caerulea Extract as Cosmetic Material 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.39 no.5, 2022년, pp.710 - 719

초록
AI-Helper

본 연구는 Passiflora caerulea 추출물의 항산화 활성 및 멜라닌 생합성 억제 효과 측정을 통해 화장품 소재로서의 활용 가능성을 확인하고자 하였다. Passiflora caerulea 추출물은 70% 에탄올로 추출하였고, TEAC assay를 이용한 ABTS radical 소거능을 측정하였다. 또한 Neutral red assay를 이용한 세포 생존율, DCF-DA를 통한 세포 내 ROS 생성 억제, 멜라닌 생합성 억제 효과를 측정하였다. 연구결과, Passiflora caerulea 추출물의 ABTS radical 소거능은 농도 의존적 소거활성이 확인되었으며, positive control(양성대조군)로 이용한 trolox와 0.1 mg/mL 농도에서는 유사한 radical 소거 활성을 확인하였다. CFDA를 통한 세포 내 활성산소종(ROS) 생성은 농도 의존적으로 억제되었고, B16F10 melanoma 세포에 대한 세포 독성은 나타나지 않았다. 또한 𝛼-MSH로 유도된 멜라닌 생합성 억제 효과를 확인하였다. 따라서 본 연구를 통해 Passiflora caerulea 추출물은 화장품 소재로서의 활용 가능성이 있는 것으로 판단된다.

Abstract ▼ AI-Helper

This study attempted to investigate the possibility of Passiflora caerulea extract as a cosmetic material by measuring its antioxidant activities and melanin biosynthesis-inhibiting effects. For this, the substance was extracted with 70% ethanol, and ABTS radical scavenging activities were measured, using the TEAC assay. In addition, cell viability through the neutral red assay, ROS inhibition and inhibition of melanin biosynthesis were analyzed, and the results found the followings: In terms of ABTS radical scavenging activities of the extract, dose-dependent scavenging activities were observed. In trolox used as a positive control group and 0.1 mg/mL, similar radical scavenging activities were found. The production of ROS through CFDA was inhibited in a dose-dependent fashion. Furthermore, inhibition of melanin biosynthesis induced by 𝛼-MSH was confirmed. Therefore, it is reasonable to conclude that Passiflora caerulea extract has a potential as a cosmetic ingredient.

주제어

표/그림 (4)

그림 Fig. 1. ABTS radical scavenging activity of Passiflora caerulea extract using TEAC assay. Three independent experiments were performed, and the results were expressed as mean± SD deviation.
그림 Fig. 2. Cell viability using Neutral red assay. In B16F10 melanoma cells, cytotoxicity was measured through cell viability of Passiflora caerulea extract. Results are presented as mean ± SD. of three independent experiments.
그림 Fig. 3. Intracellular Reactive Oxidant Species (ROS) using DCF-DA. The inhibition effect on Reactive Oxidative Species (ROS) production of the Passiflora caerulea extract in B16F10 melanoma cells was measured. Three independent trials of experiment were performed, and the results were stated in mean ± SD deviation. (*p<0.05, **p<0.01, ***p<0.001 vs. TBHP).
그림 Fig. 4. Inhibition of melanin synthesis in B16F10 melanoma cell treated with Passiflora caerulea extract. Three independent trials of experiment were performed, and the results were stated in mean ± SD deviation. (***p<0.001 vs. α-MSH)

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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