[논문]병풀 추출물이 첨가된 음료 시제품의 장내 면역조절 효과와 지표물질

김연숙; 신현영; 구자평; 하은지; 정원비; 정미연; 유광원

doi:10.9799/ksfan.2023.36.6.436

[국내논문] 병풀 추출물이 첨가된 음료 시제품의 장내 면역조절 효과와 지표물질
Intestinal Immunomodulatory Effect and Marker Compound of Centella asiatica Extracts-Added Beverage Prototype 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.6, 2023년, pp.436 - 444

김연숙 (한국교통대학교 식품영양학전공) , 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템 융합전공) , 구자평 (한국교통대학교 식품영양학전공) , 하은지 (한국교통대학교 식품영양학전공) , 정원비 (한국교통대학교 식품영양학전공) , 정미연 ((주)참선진녹즙) , 유광원 (한국교통대학교 식품영양학전공)

Abstract ▼ AI-Helper

To produce an intestinal immunomodulatory beverage containing Centella asiatica extract (CAE), three types of CAE-added beverage prototypes were prepared, and their immunomodulatory activities and marker compounds were analyzed. As a result of the cytotoxicity assessment, all the beverages did not show significant toxicity compared to the control group. Next, the immunomodulatory activities of the beverage prototype were evaluated using the inflammatory model of IL-1β-induced intestinal epithelial cell line. All the samples significantly reduced the production of IL-6, IL-8, and MCP-1 in a CAE concentration-dependent manner. In addition, CAE-added beverages inhibited NO, IL-6, and IL-12 production in LPS-induced RAW 264.7 cells. When the major triterpenoids, as marker compounds for the production of CAE-added beverages, were analyzed by HPLC-DAD, only asiaticoside was detected beyond the limit of quantification, while madecassoside, madecassic acid, and asiatic acid were not detected. The amounts of asiaticoside in CAE-added beverage prototypes were confirmed in No. 1 (19.39 ㎍/mL), 2 (19.25 ㎍/mL), and 3 (19.98 ㎍/mL). In conclusion, the results of this study suggested that CAE-added beverage prototypes induced immunomodulatory effects in the intestinal inflammatory cell line models and asiaticoside could be used as a marker compound for CAE-added beverage production.

주제어

표/그림 (7)

표 Table 1. Extraction conditions for preparing CAE-added beverages
표 Table 2. HPLC conditions for quantitative analysis of major triterpenoids in CAE-added beverages
그림 Fig. 1. Cytotoxic effect of CAE-added beverages against Caco-2 cell (A), and RAW 264.7 macrophage (B). Each beverage was treated to Caco-2 or RAW 264.7 cells, and IL-1β or LPS were subsequently stimulated for 24 h. Results are expressed as mean±S.D. of three independent tests in triplicate. Means with different letters (lowercase; stock and uppercase; dilution) above the bars are significantly different between groups at p<0.05 by Duncan's multiple range test. 1; 0.1% CAE-added beverage, 2; 1% CAE-added beverage, and 3; 2% CAE-added beverage.
그림 Fig. 2. Anti-inflammatory effect of CAE-added beverage on IL-1β-stimulated Caco-2 cell. Caco-2 cells were treated with each beverage followed by IL-1β treatment for 24 h, and the production of IL-6 (A), IL-8 (B), and MCP-1 (C) was evaluated. Results are expressed as mean±S.D. of three independent tests in triplicate. Means with different letters (lowercase; stock and uppercase; dilution) above the bars are significantly different between groups at p<0.05 by Duncan’s multiple range test. 1; 0.1% CAE-added beverage, 2; 1% CAE-added beverage, and 3; 2% CAE-added beverage.
그림 Fig. 3. Anti-inflammatory effect of CAE-added beverage on LPS-stimulated RAW 264.7 cell. RAW 264.7 cells were treated with each beverage followed by LPS treatment for 24 h, and production of NO (A), IL-6 (B), and IL-12 (C) was evaluated. Results are expressed as mean±S.D. of three independent tests in triplicate. Means with different letters (lowercase; stock and uppercase; dilution) above the bars are significantly different between groups at p<0.05 by Duncan's multiple range test. 1; 0.1% CAE-added beverage, 2; 1% CAE-added beverage, and 3; 2% CAE-added beverage.
그림 Fig. 4. HPLC chromatogram of tritepenoid standard mixture, and 2% CAE-added beverage.
표 Table 3. Contents of major triterpenoids in CAE-added beverages

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