[논문]RAW264.7 대식세포에서 표고버섯과 다시마 혼합 추출액의 항염증 효과

김수봉; 강순아

doi:10.9799/ksfan.2023.36.6.535

RAW264.7 대식세포에서 표고버섯과 다시마 혼합 추출액의 항염증 효과
Anti-Inflammatory Effects of Shiitake Mushroom and Kelp Mixture Extracts in RAW264.7 Cell 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.6, 2023년, pp.535 - 542

김수봉 (호서대학교 보건산업연구소) , 강순아 (호서대학교 벤처대학원 융합공학과)

Abstract ▼ AI-Helper

We investigated the anti-inflammatory effects of shiitake mushroom and kelp (SMK) mixture extracts in lipopolysaccharide (LPS)-stimulated murine RAW 264.7 cells. Treatment of RAW 264.7 cells with LPS significantly increased NO (nitric oxide) production, pro-inflammatory cytokines (tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-6, and IL-1β), and inflammation-related genes (COX-2 and inducible nitric oxide synthase (iNOS)). In cytotoxicity testing using RAW 264.7 cells, SMK mixture extracts in the range of 1-16 ㎍/mL did not inhibit cell proliferation. However, SMK mixture extracts significantly inhibited NO production in a dose-dependent manner (p<0.05). SMK treatment significantly decreased TNF-α, IL-6, IFN-γ, and IL-1β levels compared to the LPS group, and similarly, pro-inflammatory cytokine mRNA levels also decreased. SMK mixture extracts reduced the mRNA expression of COX-2 and iNOS in RAW 264.7 cells compared to LPS (p<0.05). The above results show that SMK mixture extracts suppressed the inflammatory response induced by LPS. In particular, the extracts were shown to regulate the inflammatory response by suppressing the expression of inflammatory cytokines and inflammation-related enzymes.

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표/그림 (4)

그림 Fig. 1. Effect of shiitake mushroom and kelp mixture extracts (SMK) on RAW 264.7 cell viability (A) and SMK on LPS-induced RAW 264.7 cell (B). LPS: treated with LPS (1 μg/mL). shiitake mushroom and kelp mixture extracts (SMK): treated with various concentrations (1, 4, and 16 μg/mL) of with LPS (1 μg/mL). ^a-dMeans with the different letters on the bars are significantly different (p<0.05) by Duncun's multiple range tests.
그림 Fig. 2. Effect of shiitake mushroom and kelp mixture extracts (SMK) on NO production in LPS-induced RAW 264.7 cell. LPS: treated with LPS (1 μg/mL). shiitake mushroom and kelp mixture extracts (SMK): treated with various concentrations (1, 4, and 16 μg/mL) of with LPS (1 μg/mL). ^a-eMeans with the different letters on the bars are significantly different (p<0.05) by Duncun's multiple range tests.
그림 Fig. 3. Effect of shiitake mushroom and kelp mixture extracts (SMK) on pro-inflammatory cytokines TNF-α, IFN-γ, IL-6 and IL-1β expression level in LPS-induced RAW 264.7 cell. LPS: treated with LPS (1 μg/mL). shiitake mushroom and kelp mixture extracts (SMK): treated with various concentrations (1, 4, and 16 μg/mL) of with LPS (1 μg/mL). ^a-eMeans with the different letters on the bars are significantly different (p<0.05) by Duncun's multiple range tests.
그림 Fig. 4. Effect of shiitake mushroom and kelp mixture extracts (SMK) on mRNA expression of TNF-α, IL-6, iNOS and COX-2 in LPS-induced RAW 264.7 cell. LPS: treated with LPS (1 μg/mL). shiitake mushroom and kelp mixture extracts (SMK): treated with various concentrations (1, 4, and 16 μg/mL) of with LPS (1 μg/mL). The mRNA expression levels were calculated based on 18S rRNA, which was used as a control(no treatment fold ratio=1). ^a-eMeans with the different letters on the bars are significantly different (p<0.05) by Duncun's multiple range tests.

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