[논문]Occurrence of Gray Mold in Wasabi Caused by Botrytis cinerea in Korea

Wan-Gyu Kim; Gyo-Bin Lee; Hong-Sik Shim; Weon-Dae Cho

doi:10.4489/kjm.20230028

Occurrence of Gray Mold in Wasabi Caused by Botrytis cinerea in Korea 원문보기

한국균학회지 = The Korean journal of mycology, v.51 no.4, 2023년, pp.259 - 263

Wan-Gyu Kim (Global Agro-Consulting Corporation) , Gyo-Bin Lee (Global Agro-Consulting Corporation) , Hong-Sik Shim (Global Agro-Consulting Corporation) , Weon-Dae Cho (Global Agro-Consulting Corporation)

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During crop disease surveys in 2019 and 2021, we observed gray mold symptoms on the leaves of wasabi (Eutrema japonicum) plants grown in vinyl greenhouses in Taebaek and Pyeongchang, Gangwon Province, Korea. The symptoms appeared at the edges of the leaves, where the infected areas turned black and rotted, and many gray molds formed on the lesions. The incidence of gray mold on the leaves in the vinyl greenhouses at the two locations ranged from 1 to 30%. Four fungal isolates were obtained from leaf lesions and identified as Botrytis cinerea based on morphological characteristics and phylogenetic analysis. Two isolates of B. cinerea were used for pathogenicity tests on the leaves of wasabi plants by artificial inoculation. The pathogenicity of the isolates was confirmed in leaves. The symptoms induced by the isolates were similar to those observed in wasabi leaves in the vinyl greenhouses investigated. This is the first report of B. cinerea causing gray mold in wasabi in Korea.

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그림 Fig. 1. Gray mold symptoms of wasabi plants. (A and B) Symptoms observed on the leaves in the investigated vinyl greenhouse. (C) Symptoms on the leaves induced by artificial inoculation test with Botrytis cinerea isolate (EJBC-2102). (D) A non-inoculated plant (control).
표 Table 1. Occurrence of gray mold on wasabi leaves in vinyl greenhouses located in Taebaek and Pyeongchang, Korea in 2019 and 2021
그림 Fig. 2. Cultural and morphological features of Botrytis cinerea isolates from diseased wasabi leaves. (A) A colony of an isolate (EJBC-2102) grown on potato dextrose agar at 20°C for 25 days. (B) A conidiophore and conidia. .
그림 Fig. 3. Phylogenetic tree based on glyceraldehyde-3-phosphate dehydrogenase, heat-shock protein 60, and DNA-dependent RNA polymerase subunit II gene regions of the four isolates (EJBC-1901, EJBC-1902, EJBC- 2101, and EJBC-2102) from wasabi and reference strains of Botrytis species. Sequences of the reference species were obtained from the NCBI GenBank database. The phylogenetic tree was generated using the neighborjoining method with a maximum composite likelihood model. The bootstrap support values are shown at the nodes. The scale bar represents the number of nucleotide substitutions per site.

참고문헌 (10)

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Occurrence of Gray Mold in Wasabi Caused by Botrytis cinerea in Korea 원문보기

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