[논문]토양에서 분리한 자낭균 효모 Wickerhamomyces sp. GW1-4와 Archaeorhizomyces sp. YB4-103의 특성

손지윤; 김명겸

doi:10.4489/kjm.20230036

[국내논문] 토양에서 분리한 자낭균 효모 Wickerhamomyces sp. GW1-4와 Archaeorhizomyces sp. YB4-103의 특성
Characterization of Ascomycetous Yeast Species Wickerhamomyces sp. GW1-4 and Archaeorhizomyces sp. YB4-103 isolated from Soil 원문보기

한국균학회지 = The Korean journal of mycology, v.51 no.4, 2023년, pp.361 - 371

손지윤 (서울여자대학교 생명환경공학과) , 김명겸 (서울여자대학교 생명환경공학과)

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The study was undertaken to isolate and characterize wild yeast strains from soil samples collected in Seoul, Korea. Among the 19 yeast strains obtained, 17 were previously recorded species. The remaining two strains, Wickerhamomyces sp. GW1-4 and Archaeorhizomyces sp. YB4-103 were new species candidates. The genomic and microbiological characteristics of GW1-4 and YB4-103 were investigated. Phylogenetic analyses based on the 26S rRNA gene sequences and internal transcribed sequences, GW1-4 and YB4-103, represent a distinct lineage within the family Phaffomycetaceae and Archaeorhizomycetaceae, respectively. The GW1-4 and YB4-103 strains had the highest sequence homology with Wickerhamomyces xylosivorus NBRC 111553T (88.97%) and Archaeorhizomyces finlayi CBS 128710T (87.55%), respectively.

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표/그림 (7)

표 Table 1. A list of wild yeasts strains isolated from soil samples of Mt. Buramsan in Seoul, Korea.
그림 Fig. 1. Neighbor-joining phylogenetic tree based on the nucleotide sequences of D1/D2 region of 26S rDNA showing the position of novel yeast GW1-4. The tree was generated by the neighbor-joining method, using MEGA11. Bootstrap values (>70%) are shown at the branch nodes. Bar, 0.02 substitutions per nucleotide position. Saccharomyces cerevisiae NRRL Y-12632^T was used as an outgroup. ^Ttype strain.
그림 Fig. 2. Neighbor-joining phylogenetic tree based on the nucleotide sequences of D1/D2 region of 26S rDNA showing the position of novel yeast YB4-103. The tree was generated by the neighbor-joining method, using MEGA11. Bootstrap values (>70%) are shown at the branch nodes. Bar, 0.05 substitutions per nucleotide position. Phillipsia gelatinosa MFLU 15-2360^T was used as an outgroup. ^Ttype strain.
그림 Fig. 3. Phylogenetic tree based on the concatenated sequences of the internal transcribed spacer (ITS) regions and D1/D2 region of the large subunit (LSU) rRNA gene and constructed by the neighbor-joining method, showing relationships of a novel species candidate GW1-4 and closely related species. Saccharomyces cerevisiae CBS 1171^T was used as an outgroup. Bootstrap values (>70%) are shown at the branch nodes. Accession numbers were shown in parentheses. Bar, 0.05 substitutions per nucleotide position.^Ttype strain.
그림 Fig. 4. Phylogenetic tree based on the concatenated sequences of the internal transcribed spacer (ITS) regions and D1/D2 region of the large subunit (LSU) rRNA gene and constructed by the neighbor-joining method, showing relationships of a novel species candidate YB4-103 and closely related species. Phillipsia gelatinosa MFLU 15-2360^T was used as an outgroup. Bootstrap values (>70%) are shown at the branch nodes. Accession numbers were shown in parentheses. Bar, 0.05 substitutions per nucleotide position.^Ttype strain.
표 Table 2. Mycological characteristics of Wickerhamomyces sp. GW1-4 and Archaeorhizomyces sp. YB4-103.
그림 Fig. 5. Morphology of cells from strain GW1-4 and strain YB4-103 incubated at 25℃. All strains were grown after 3 days on yeast extract peptone dextrose (YPD) agar. The budding cells of Wickerhamomyces sp. GW1-4 (A) and Archaeorhizomyces sp. YB4-103 (B). bars, 10 μm. The colonies of Wickerhamomyces sp. GW1-4 (C) and Archaeorhizomyces sp. YB4-103 (D).

참고문헌 (15)

Ahn CR, Kim SN, Kim CG. Taxonomic study on six yeast species unlisted in the national？species list of Korea. Kor J Mycol 2023;51:7-24.
Kim JY, Lee SY, Han SM, Lee JS. Production of anti-dementia acetylcholinesterase inhibitors？from the wild yeasts Saccharomyces cerevisiae WJSL0113 and Wickerhamomyces anomalus？JSF0128. Kor J Mycol 2018;46:447-51.
Chang CF, Hsu IM, Liu CH, Lee FL. Wickerhamomyces sinyiensis f.a., sp. nov., a new？ascomycetous yeast species in the Wickerhamomyces clade isolated in Taiwan. Int J Syst Evol？Microbiol 2022;72:005466.

상세보기
Kim JS, Lee MR, Kim JY, Heo J, Kwon SW, Yun BS, Kim SJ. Distribution and species？diversity of wild yeasts isolated from flowers in Korea. Kor J Mycol 2020;48:375-484.
Menkis A, Urbina H, James YT, Rosling A. Archaeorhizomyces borealis sp. nov. and a？sequence based classification of related soil fungal species. Fungal Biol 2014;118:943-55.

상세보기
Kim JY, Jung HY, Park JS, Cho SJ, Lee HB, Sung GH, Subramani G, Kim MK. Isolation？and characterization of cellulolytic yeast belonging to Moesziomyces sp. from the gut of？Grasshopper. Kor J Microbiol 2019;55:234-41.
Kurtzman CP, Robnett CJ. Identification and phylogeny ascomycetous yeasts from analysis？of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek？1998;73:331-71.
Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X？windows interface: flexible strategies for multiple sequence alignment aided by quality？analysis tools. Nucleic Acids Res 1997;25:4876-82.

상세보기
Tamura K, Stecher G, Kumar S. MEGA11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 2021;38:3022-7.

상세보기
White TJ, Bruns T, Lee S, Taylor JW. Amplification and direct sequencing of fungal ribosomal？RNA genes for phylogenetics. In: PCR Protocols: A Guide to Methods and Applications. New？York: Academic Press; 1990. p. 315-22.
Gales AC, Pfaller MA, Houston AK, Joly S, Sullivan DJ, Coleman DC, Soll DR. Identification？of Candida dubliniensis based on temperature and utilization of xylose and a-methyl-D-glucoside as determined with the API 20C AUX and Vitek YBC systems. J Clin Microbiol？1999;37:3804-8.
Kurtzman CP, Robnett CJ. Identification of clinically important ascomycetous yeasts based？on nucleotide divergence in the 59 end of the large-subunit (26S) ribosomal DNA gene. J Clin？Microbiol 1997;35:1216-23.
Han SM, Kim HK, Lee HB, Lee JS. Isolation and identification of wild yeasts from？freshwaters and soils of Nakdong and Yeongsan River, Korea, with characterization of two？unrecorded yeasts. Kor J Mycol 2016;44:350-4.
Kobayashi R, Kanti A, Kawasaki H. Three novel species of D-xylose-assimilating？yeasts, Barnettozyma xylosiphila sp. nov., Barnettozyma xylosica sp. nov. and Wickerhamomycesxylosivorus f.a., sp. nov. Int J Syst Evol Microbiol 2017;67:3971-6.

상세보기
Liu XZ, Wang QM, Goker M, Groenewald M, Kachalkin AV, Lumbsch HT, Millanes AM,？Wedin M, Yurkov AM, Boekhout T, et al. Towards an integrated phylogenetic classification of？the Tremellomycetes. Stud Mycol 2015;81:85-147.？

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[국내논문] 토양에서 분리한 자낭균 효모 Wickerhamomyces sp. GW1-4와 Archaeorhizomyces sp. YB4-103의 특성
Characterization of Ascomycetous Yeast Species Wickerhamomyces sp. GW1-4 and Archaeorhizomyces sp. YB4-103 isolated from Soil 원문보기

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[국내논문] 토양에서 분리한 자낭균 효모 Wickerhamomyces sp. GW1-4와 Archaeorhizomyces sp. YB4-103의 특성 Characterization of Ascomycetous Yeast Species Wickerhamomyces sp. GW1-4 and Archaeorhizomyces sp. YB4-103 isolated from Soil 원문보기

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[국내논문] 토양에서 분리한 자낭균 효모 Wickerhamomyces sp. GW1-4와 Archaeorhizomyces sp. YB4-103의 특성
Characterization of Ascomycetous Yeast Species Wickerhamomyces sp. GW1-4 and Archaeorhizomyces sp. YB4-103 isolated from Soil 원문보기

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