[논문]알코올성 간 손상 동물 모델에서 芍藥 葛根 복합물의 간 손상 보호 효과

최정원; 김진영; 신미래; 박해진

doi:10.6116/kjh.2023.38.1.31.

알코올성 간 손상 동물 모델에서 芍藥葛根 복합물의 간 손상 보호 효과
Protective effects of Paeoniae Radix Alba and Puerariae Radix combination on alcoholic liver disease 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.38 no.1, 2023년, pp.31 - 43

최정원 (대구한의대학교 한의과대학 본초약리학교실) , 김진영 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 박해진 (대구한의대학교 DHU 바이오융복합시험센터)

Abstract ▼ AI-Helper

Objective : Alcoholic liver disease (ALD) is caused by excess alcohol intake. In the liver, alcohol breakdown results formation of toxic byproducts that lead to damage to tissue. This study is to investigate the therapeutic effects of Paeoniae Radix Alba and Puerariae Radix combination (PP) on ALD. Methods : PP was analyzed for polyphenolic compounds and free radical scavenging activity. ALD mouse model was induced by feeding ethanol and water (Control), silymarin (50 mg/kg), low-dose (PP: 100 mg/kg) or high-dose (PP: 200 mg/kg) was orally administrated to ALD mice for 14 days. The serum was assessed with levels of AST, ALT, total bilirubin, total cholesterol, and triglyceride. Liver tissues were evaluated for ROS levels, degree of liver damage and protein expression. Results : The 3:1 (Paeoniae Radix Alba:Puerariae Radix) ratio showed the best antioxidant values for the experiment. In ALD model, levels of AST, ALT, total bilirubin, total cholesterol, and triglyceride were significantly increased in the Control and the levels were decreased by treatment of PP. In addition, increased ROS, ONOO- and MDA levels in the Control were reduced in the PP groups. Western blot analysis figured out that proteins related to ROS and cholesterol metabolism were higher in ALD than in PP-treated ALD. Antioxidant enzyme expression was low in the control group and increased by PP treatment. Conclusion : Our results suggest that PP has the potential to be a medicine in ALD in terms of regulating oxidative stress and adjusting lipid metabolism.

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표/그림 (10)

표 Table 1. Total Polyphenol and Total Flavonoid Contents of Paeoniae Radix Alba and Puerariae Radix Mixture.
표 Table 2. DPPH and ABTS Radical Scavenging Activities of Paeoniae Radix Alba and Puerariae Radix Mixture.
표 Table 3. The Effect of PP on Body Weight, Liver Weight, and Relative Liver Weight.
그림 Figure 1. The effect of PP on the levels of AST, ALT, and total bilirubin in serum. AST, aspartate aminotransferase; ALT, alanine aminotransferase; PPL, the mixture of Paeoniae Radix Alba and Puerariae Radix (100 mg/kg); PPH, the mixture of Paeoniae Radix Alba and Puerariae Radix (200 mg/kg). The values were shown as mean±SD (n=7). ^#p<0.05 and ^###p<0.001 vs. Normal, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. Control.
그림 Figure 3. The effect of PP on the levels of total cholesterol and triglyceride in serum. PPL, the mixture of Paeoniae Radix Alba and Puerariae Radix (100 mg/kg); PPH, the mixture of Paeoniae Radix Alba and Puerariae Radix (200 mg/kg). The values were shown as mean±SD (n=7). ^###p < 0.001 vs. Normal, *p < 0.05 and ***p < 0.001 vs. Control.
그림 Figure 4. Histological analysis. Liver tissue were stained with hematoxylin & eosin (H&E) or Oil red O (ORO) to analyze the liver damage from alcohol (magnification, X400). PPL, the mixture of Paeoniae Radix Alba and Puerariae Radix (100 mg/kg); PPH, the mixture of Paeoniae Radix Alba and Puerariae Radix (200 mg/kg).
그림 Figure 5. Expression of proteins to stimulate NADPH oxidase in liver tissue. PPL, the mixture of Paeoniae Radix Alba and Puerariae Radix (100 mg/kg); PPH, the mixture of Paeoniae Radix Alba and Puerariae Radix (200 mg/kg). NOX2, NADPH oxidase 2; Rac 1/2/3, rasrelated C3 botulinum toxin substrate 1/2/3. ^#p < 0.05 and ^##p < 0.01 vs. Normal, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. Control.
그림 Figure 6. Expression of antioxidant enzymes in liver tissue. PPL, the mixture of Paeoniae Radix Alba and Puerariae Radix (100 mg/kg); PPH, the mixture of Paeoniae Radix Alba and Puerariae Radix (200 mg/kg). SOD-1, superoxide dismutase-1; GPx1/2, glutathione peroxidase1/2. ^##p < 0.01 and ^###p < 0.001 vs. Normal, *p < 0.05 vs. Control.
그림 Figure 7. Expression of proteins to control cholesterol homeostasis in liver tissue. PPL, the mixture of Paeoniae Radix Alba and Puerariae Radix (100 mg/kg); PPH, the mixture of Paeoniae Radix Alba and Puerariae Radix (200 mg/kg). SREBP-2, sterol regulatory element-binding protein 2; HMGCR, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase. ^#p < 0.05 and ^###p < 0.001 vs. Normal, *p < 0.05 and **p < 0.01 vs. Control.
그림 Figure 8. Expression of proteins to regulate free fatty acids in liver tissue. PPL, the mixture of Paeoniae Radix Alba and Puerariae Radix (100 mg/kg); PPH, the mixture of Paeoniae Radix Alba and Puerariae Radix (200 mg/kg). PPARα, peroxisome proliferator-activated receptor alpha; SREBP-1, sterol regulatory element-binding transcription factor 1; FAS, fatty acid synthase; SCD-1, stearoyl-CoA desaturase-1. ^##p < 0.01 and ^###p < 0.001 vs. Normal, *p < 0.05, **p < 0.01, and ***p < 0.001 vs. Control.

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