구아바 잎 추출물 및 컬럼크로마토그래피를 이용한 메탄올 분획물의 항산화 활성 Antioxidant activity of ethanol extract and methanol fractions via column chromatography from Psidium guajava Leaf원문보기
본 실험에서는 에탄올로 추출한 Psidium guajava 잎과 컬럼크로마토그래피를 이용한 메탄올 분획물의 항산화 능력을 측정하기 위해 DPPH, ABTS 라디컬소거능법, 총 페놀, 플라보노이드법, SOD assay법을 활용하였다. 항산화 활성 결과에서 40, 60% 메탄올 분획물이 의미있는 측정값을 나타냈고, 두 분획물을 선정하여 HPLC와 핵자기공명(NMR)을 활용한 화학적 분석을 통해 주요 성분의 분리 및 물질탐색을 진행하였다. HPLC 정제를 통해 quercitrin (1)와 isoquercetin (2) 두 종류의 quercetin 유도체를 분리 및 구조 동정하였다. SOD assay 결과에서 컬럼크로마토그래피법을 활용하여 얻은 몇몇의 분획물과 단일물질로 분리한 화합물들은 추출물보다 향상된 항산화 활성을 보였다.
본 실험에서는 에탄올로 추출한 Psidium guajava 잎과 컬럼크로마토그래피를 이용한 메탄올 분획물의 항산화 능력을 측정하기 위해 DPPH, ABTS 라디컬소거능법, 총 페놀, 플라보노이드법, SOD assay법을 활용하였다. 항산화 활성 결과에서 40, 60% 메탄올 분획물이 의미있는 측정값을 나타냈고, 두 분획물을 선정하여 HPLC와 핵자기공명(NMR)을 활용한 화학적 분석을 통해 주요 성분의 분리 및 물질탐색을 진행하였다. HPLC 정제를 통해 quercitrin (1)와 isoquercetin (2) 두 종류의 quercetin 유도체를 분리 및 구조 동정하였다. SOD assay 결과에서 컬럼크로마토그래피법을 활용하여 얻은 몇몇의 분획물과 단일물질로 분리한 화합물들은 추출물보다 향상된 항산화 활성을 보였다.
The antioxidant capacity of the Psidium guajava leaf extracted with EtOH and their MeOH fractions using column chromatography were evaluated by 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging assays, total phenolic and flavonoid content, and...
The antioxidant capacity of the Psidium guajava leaf extracted with EtOH and their MeOH fractions using column chromatography were evaluated by 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging assays, total phenolic and flavonoid content, and Superoxide dismutase (SOD) assay. To determine its utility as a functional material, the crude extract was fractionated by flash column chromatography on ODS using a stepwise elution with combinations of MeOH/H2O and then all the fractions were also investigated. In the results of antioxidant activities, the 40% and 60% MeOH fractions show the meaningful values, and then the two fractions were selected to examine the isolation and identification of the major constituents via HPLC and nuclear magnetic resonance. Further purification led to isolation of two quercetin derivatives; quercitrin (1) and isoquercetin (2). Through SOD assay, some methanol fractions via column chromatography and isolated compounds showed improved antioxidant activities compared to the extract.
The antioxidant capacity of the Psidium guajava leaf extracted with EtOH and their MeOH fractions using column chromatography were evaluated by 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid radical scavenging assays, total phenolic and flavonoid content, and Superoxide dismutase (SOD) assay. To determine its utility as a functional material, the crude extract was fractionated by flash column chromatography on ODS using a stepwise elution with combinations of MeOH/H2O and then all the fractions were also investigated. In the results of antioxidant activities, the 40% and 60% MeOH fractions show the meaningful values, and then the two fractions were selected to examine the isolation and identification of the major constituents via HPLC and nuclear magnetic resonance. Further purification led to isolation of two quercetin derivatives; quercitrin (1) and isoquercetin (2). Through SOD assay, some methanol fractions via column chromatography and isolated compounds showed improved antioxidant activities compared to the extract.
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