[논문]육계(Cortex of Cinnamomum cassia J.Presl) 추출물로부터 부정근 형성 유도물질 분리 및 동정

여주호; 백정규; 박지성; 김건우

doi:10.7732/kjpr.2024.37.1.011

육계(Cortex of Cinnamomum cassia J.Presl) 추출물로부터 부정근 형성 유도물질 분리 및 동정
Isolation and Identification of Adventitious Root Formation Inducing Substances from Cortex of cinnamomum cassia J.Presl

韓國資源植物學會誌 = Korean journal of plant resources, v.37 no.1, 2024년, pp.11 - 21

여주호 (국립안동대학교 생약자원학과) , 백정규 (부부한의원) , 박지성 ((주)한국식물환경연구소) , 김건우 (국립안동대학교 생약자원학과)

초록
AI-Helper

본 연구에서 육계 추출물로부터 cinnamyl alcohol을 분리·정제하였다. 연구 결과, H3 분획100 ㎍/mL에서 부정근 형성율이 286.6%였으며, 표준품 cinnamyl alcohol 1 mM (134.2 ㎍/mL)의 형성율이 290%로 유사한 결과를 나타내었다. 따라서 육계 methanol 추출물에 함유되어있는 cinnamyl alcohol이 녹두의 부정근 형성을 유도하는 주된 화합물일 것으로 판단된다. 육계에서 동정한 cinnamyl alcohol은 현재 식품첨가제 및 향료로써 사용되고 있으므로 안전성이 이미 보장되어 있으며, 한약재로써 사용된 육계 잔류물을 활용하여 부정근 형성 유도물질을 추출한다면비용적인 측면에서 또한 긍정적인 요인으로 작용할 것으로 판단된다. 따라서 육계는 유기농 농자재로써 충분히 사용 가능할 뿐만 아니라 신규 천연 식물생장조절제의 개발용 소재로서도 활용 가능할 것으로 사료된다.

Abstract ▼ AI-Helper

In this study, as a result of exploring the physiological activity of plants useful for agriculture on various plant resources, it was possible to confirm an activity similar to auxin that promotes plant rooting in methanol extract of Cinnamon Bark (cortex of Cinnamomum cassia J.Presl). After separating the active body by applying column chromatography and HPLC to the CHCl3 active fraction obtained by solvent extraction for each polarity from the methanol extract of cinnamon bark, cinnamyl alcohol was identified through GC/MS analysis. By bioassay using cinnamyl alcohol standard and the active fraction separated and purified from the methanol extract of cinnamon bark, the rooting rate of mung bean seedlings of the cinnamyl alcohol standard was 290% compared with the untreated control at 134.2 ㎍/mL concentration, and the adventitious root formation activity similar to the rooting rate (268.6%; 100 ㎍/mL) of the active fraction was shown. In conclusion, it is believed that cinnamyl alcohol contained in methanol extract of Cinnamon Bark is the main compound that induces adventitious root formation in mung bean.

주제어

표/그림 (17)

그림 Fig. 1. Schematic isolation procedure of substances inducing adventitious roots formation from Cinnamon Bark 80% methanol extracts.
$Fig. 2. Schematic isolation procedure of adventitious roots formation induced compounds from the purified chloroform fraction.$ 그림 Fig. 2. Schematic isolation procedure of adventitious roots formation induced compounds from the purified chloroform fraction.
그림 Fig. 3. The amount of Cinnamon Bark extract by methanol concentration.
표 Table 1. The condition of GC/MS for the identification of adventitious roots formation inducing substances.
그림 Fig. 4. Effect of Cinnamon Bark extract by methanol concentration on the formation of adventitious roots. A, Control; B, 0% MeOH; C, 20% MeOH; D, 40% MeOH; E, 60% MeOH; F, 80% MeOH. The vertical bar represents the mean ± S.E. of three replicates. *p < 0.05 compared to the control.
그림 Fig. 5. Effect of concentration-specific treatment of 80% methanol extract from Cinnamon Bark on adventitious roots formation. A: Control, B: 0.25 mg/mL, C: 0.5 mg/mL, D: 1 mg/mL, E: 2 mg/mL, F: 4 mg/mL. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.
그림 Fig. 6. Adventitious roots formation effect of various organic solvent extracts from Cinnamon Bark extract. A: Control, B: 80% MeOH extract, C: Hexane, D: Chloroform, E: EtOAC, F: MeOH, G: Residues. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.
$Fig. 9. Semi-prep. HPLC chromatogram of CS-4 fraction purified from Cinnamon Bark.$ 그림 Fig. 9. Semi-prep. HPLC chromatogram of CS-4 fraction purified from Cinnamon Bark.
$Fig. 10. Effect of H fractions purified from CS-4 fraction on adventitious roots formation. A: Control, B: CS-4, C: H1, D: H2, E: H3, F: H4. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.$ 그림 Fig. 10. Effect of H fractions purified from CS-4 fraction on adventitious roots formation. A: Control, B: CS-4, C: H1, D: H2, E: H3, F: H4. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.
$Fig. 7. Effect of CF fractions purified from chloroform extract on adventitious roots formation. A: Control, B: Chloroform, C: CF-1, D: CF-2, E: CF-3, F: CF-4, G: CF-5, H: CF-6. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.$ 그림 Fig. 7. Effect of CF fractions purified from chloroform extract on adventitious roots formation. A: Control, B: Chloroform, C: CF-1, D: CF-2, E: CF-3, F: CF-4, G: CF-5, H: CF-6. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.
$Fig. 8. Effect of CS fractions purified from CF-5 fraction on adventitious roots formation. A: Control, B: CF-5, C: CS-1, D: CS-2, E: CS-3, F: CS-4, G: CS-5. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.$ 그림 Fig. 8. Effect of CS fractions purified from CF-5 fraction on adventitious roots formation. A: Control, B: CF-5, C: CS-1, D: CS-2, E: CS-3, F: CS-4, G: CS-5. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.
$Fig. 11. HPLC chromatogram of cinnamyl alcohol and H3 fraction purified from Cinnamon Bark. A: Standard (Cinnayl alcohol), B: H3 fraction.$ 그림 Fig. 11. HPLC chromatogram of cinnamyl alcohol and H3 fraction purified from Cinnamon Bark. A: Standard (Cinnayl alcohol), B: H3 fraction.
$Fig. 12. Gas chromatogram of H3 fraction purified from Cinnamon Bark.$ 그림 Fig. 12. Gas chromatogram of H3 fraction purified from Cinnamon Bark.
그림 Fig. 13. Mass spectrum and chemical structure of 2-propen-1-ol, 3-phenyl- identified from the active farction H3 of Cinnamon Bark extracts.
그림 Fig. 14. Effect of treatment by concentration of standard product cinnamyl alcohol on the formation of adventitious roots. A: Control, B: 0.1 mM, C: 0.5 mM, D: 1 mM, E: 2 mM. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.
그림 Fig. 15. Effect of Cinnamon Bark hot water extract by concentration on the formation of adventitious roots. A: Control, B: 0.25 mg/mL, C: 0.5 mg/mL, D: 1 mg/mL, E: 2 mg/mL, F: 4 mg /mL. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.
그림 Fig. 16. Effect of 80% methanol extract from Cinnamon Bark hot water extraction residues on adventitious roots formation. A: Control, B: 0.25 mg/mL, C: 0.5 mg/mL, D: 1 mg/mL, E: 2 mg /mL, F: 4 mg/mL. The vertical bar represents the mean ± S.E. of three replicates. *P < 0.05 compared to the control.

참고문헌 (31)

Api, A.M., D.S. Belsito, D. Biserta, Botelho, M. Bruze, Jr？Burton, J. Buschmann, M.A. Cancellieri, M.L. Dagli, M. Date,？W. Dekant, C. Deodhar, A.D. Fryer, S. Gadhia, L. Jones, K.？Joshi, A. Lapczynski, M. Lavelle, D.C. Liebler, M. Na, D.O'？Brien, A. Patel, T.M. Penning, G. Ritacco, F. Rodriguez-Ropero, J. Romine, N. Sadekar, D. Salvito, T.W. Schultz, F.？Siddiqi, I.G. Sipes, G. Sullivan, Y. Thakkar, Y. Tokura and？S. Tsang. 2020. RIFM fragrance ingredient safety assessment,？cinnamyl alcohol, CAS Registry Number 104-54-1. Food？Chem. Toxicol. 141:111337.

상세보기
Bagni, N., B. Malucelli and P. Torrigiani. 1980. Polyamines,？storage substances and abscisic acid-like inhibitors during？dormancy and very early activation of Helianthus tuberosus？tuber tissues. Physiol. Plant. 49(4):341-345.

상세보기
Choi, J., K.T. Lee, H. Ka, W.T. Jung, H.J. Jung and H.J. Park. 2001. Constituents of the essential oil of the Cinnamomum？cassia stem bark and the biological properties. Arch. Pharm.？Res. 24(5):418-423.

원문보기 상세보기
Choi, Y.H. 1982. Phytonematology, Hyang-moon-sa, Korea. pp.？58-69.
Evans, M.L., H. Ishikawa and M.A. Estelle. 1994. Responses of？Arabidopsis roots to auxin studied with high temporal resolution: comparison of wild type and auxin-response mutants.？Planta. 194(2):215-222.

상세보기
Geiss, G., L. Gutierrez and C. Bellini. 2009. Adventitious root？formation: New insights and perspectives. Annu. Plant Rev.？Online. 37:127-156.
Guilfoyle, T.J. and J.L. Key. 1986. Auxin-regulated gene expression？in higher plants. Crit. Rev. Plant Sci. 4(3):247-276.

상세보기
Hess, C.E. 1961. Physiology of root initiation in easy-and？difficult-to-root cuttings. The Hormolog. 3:3-6.
Hyun, Y.H., J. Choi and W.S. Shin. 2021. Removal of 2, 4-D by？an Fe (II)/persulfate/electrochemical oxidation process. J.？Soil Groundwater Environ. 26(1):45-53.
Jaafarzadeh, N., F. Ghanbari and A. Zahedi. 2018. Coupling？electrooxidation and oxone for degradation of 2, 4-Dichlorophenoxyacetic acid (2, 4-D) from aqueous solutions. J.？Water Process. Eng. 22:203-209.
Kashiwada, Y.,T. Nohara, T. Tomimatsu and I. Nishioka. 1981.？Constituents of cinnamomi cortex. IV. Structures of cinncassiols C1 glucoside, C2 and C3. Chem. Pharm. Bull. 29(9):2686-2688.

상세보기
Lebuhn, M., T. Heulin and A. Hartmann. 1997. Production of auxin？and other indolic and phenolic compounds by Paenibacillus？polymyxa strains isolated from different proximity to plant？roots. FEMS Microbiol. Ecol. 22(4):325-334.
Lee, E. 2015. Effect of cinnamon extract on the inflammatory？response in the LPS-shock rat. Korean J. Plant Res. 28(3):333-340.

원문보기 상세보기
Lee, J.D., H.B. Hyun, H. Hyeon, E. Jang, M.H. Ko, W.J. Yoon,？Y.M. Ham, Y.H. Jung, H. Choi, E.G.O and D. Oh. 2022. Mass？proliferation of Hibiscus hamabo adventitious root in an airlift bioreactor, and the antioxidant and whitening activity of？the extract. Korean J. Plant Res. 35(4):435-444.
Letizia, C.S., J. Cocchiara, J. Lalko, A. Lapczynski and A.M.？Api. 2005. Fragrance material review on cinnamyl alcohol.？Food Chem. Toxicol. 43(6):837-866.

상세보기
Lin, C.C., S.J. Wu, C.H. Chang and L.T. Ng. 2003. Antioxidant？activity of Cinnamomum cassia. Phytother. Res. 17(7):726-730.
Lockwood, G.B. 1979. The major constituents of the essential？oils of Cinnamomum cassia Blume growing in Nigeria. Planta？Med. 36(8):380-381.

상세보기
Loomis, D., K. Guyton, Y. Grosse, F. El Ghissasi, V. Bouvard,？L. Benbrahim-Tallaa, G. Neela, M. Heidi and S. Kurt. 2015.？International agency for research on cancer monograph？working group. Carcinogenicity of lindane, DDT, and 2,？4-dichlorophenoxyacetic acid. Lancet. Oncol. 16(8):891-892.
Mattia, A. and G.I. Sipes. 2001. Cinnamyl alcohol and related？flavouring agents. Safety evaluation of certain food additives？and contaminants. WHO Food AD. 46:994-1010.
Morimoto, S., G.I. Nonaka and I. Nishioka. 1986a. Tannins and？related compounds. XXXVIII.: Isolation and characterization？of flavan-3-ol glucosides and procyanidin oligomers from Cassia bark: Cinnamomum cassia BLUME. Chem. Pharm.？Bull. 34(2):633-642.

상세보기
Morimoto, S., G.I. Nonaka and I. Nishioka. 1986b. Tannins and related compounds.？XXXIX.: Procyanidin C-glucosides and an acylated flavan3-ol glucoside from the barks of Cinnamomum cassia BLUME？and C. obtusifolium NEES. Chem. Pharm. Bull. 34(2):643-649.

상세보기
Nohara, T., I. Nishioka, N. Tokubuchi, K. Miyahara and T.？Kawasaki. 1980a. Cinncassiol C1, a novel type of diterpene？from cinnamomi cortex. Chem. Pharm. Bull. 28(6):1969-1970.

상세보기
Nohara, T., N. Tokubuchi, M. Kuroiwa and I. Nishioka. 1980b.？The constituents of cinnamomi cortex. III. Structures of？cinncassiol B and its glucoside. Chem. Pharm. Bull. 28(9):2682-2686.

상세보기
Nohara, T., Y. Kashiwada and I. Nishioka. 1985. Cinncassiol E,？a diterpene from the bark of Cinnamomum cassia. Phytochemistry 24(8):1849-1850.

상세보기
Nohara, T., Y. Kashiwada, K. Murakami, T. Tomimatsu, M.？Kido, A. Yagi and I. Nishioka. 1981. Constituents of cinnamomi cortex. V. Structures of five novel diterpenes, cinncassiols D1, D1 glucoside, D2, D2 glucoside and D3. Chem.？Pharm. Bull. 29(9):2451-2459.

상세보기
Nohara, T., Y. Kashiwada, T. Tomimatsu and I. Nishioka. 1982.？Two novel diterpenes from bark of Cinnamomum cassia.？Phytochemistry 21(8):2130-2132.

상세보기
Ooi, L.S., Y. Li, S.L. Kam, H. Wang, E.Y. Wong and V.E. Ooi.？2006. Antimicrobial activities of cinnamon oil and cinnamaldehyde from the Chinese medicinal herb Cinnamomum？cassia Blume. Am. J. Chinese Med. 34(03):511-522.
Sagara, K., T. Oshima, T. Yoshida, T. Yu-Yi, Z. Guande and C.？Yu-Heng. 1987. Determination of Cinnamomi cortex by？high-performance liquid chromatography. J. Chromatogr. A.？409:365-370.

상세보기
Shiraga, Y., K. Okano, T. Akira, C. Fukaya, K. Yokoyama, S.？Tanaka, Fukui H. and M. Tabata. 1988. Structures of potent？antiulcerogenic compounds from Cinnamomum cassia. Tetrahedron 44(15):4703-4711.

상세보기
Verspohl, E.J., K. Bauer and E. Neddermann. 2005. Antidiabetic effect of Cinnamomum cassia and Cinnamomum？zeylanicum in vivo and in vitro. Phytother. Res. 19(3):203-206.
Yagi, A., N. Tokubuchi, T. Nohara, G. Nonaka, I. Nishioka and？A. Koda. 1980. The constituents of Cinnamomi cortex. I.？structures of cinncassiol A and its glucoside. Chem. and？Pharm. Bull. 28(5):1432-1436.

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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