[논문]고온 환경에서 카테킨 및 비타민 C 첨가가 육계의 생산성, 계육품질, 혈액성분 및 스트레스 지표에 미치는 영향

손지선; 이우도; 김희진; 김현수; 홍의철; 전익수; 강환구

doi:10.5536/kjps.2023.50.1.1

고온 환경에서 카테킨 및 비타민 C 첨가가 육계의 생산성, 계육품질, 혈액성분 및 스트레스 지표에 미치는 영향
Effect of Combined Supplementation Catechin and Vitamin C on Growth Performance, Meat Quality, Blood Composition and Stress Responses of Broilers under High Temperature 원문보기

한국가금학회지 = Korean journal of poultry science, v.50 no.1, 2023년, pp.1 - 13

손지선 (국립축산과학원 가금연구소) , 이우도 (국립축산과학원 가금연구소) , 김희진 (국립축산과학원 가금연구소) , 김현수 (국립축산과학원 가금연구소) , 홍의철 (국립축산과학원 가금연구소) , 전익수 (국립축산과학원 가금연구소) , 강환구 (국립축산과학원 가금연구소)

초록
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본 연구는 고온환경에 노출된 육계의 사료 내 카테킨과 비타민 C의 단독 및 혼합 첨가에 따른 생산성, 계육품질, 혈액 성상 및 스트레스 지표에 미치는 영향을 조사하기 위해 수행하였다. 21일령 육계(Ross 308) 수컷 360수를 공시하였으며, 처리구는 비타민 C(0, 250 mg/kg)와 카테킨(0, 600, 1,200 mg/kg)로 2 × 3요인으로 나누었으며, 처리당 6반복, 반복당 10수를 완전임의 배치하였다. 고온 환경은 32±1℃로, 습도는 60±5%를 유지하여 시험종료까지 24시간동안 노출시켰다. 카테킨과 비타민 C의 처리에 따른 증체량, 섭취량 및 사료요구율은 유의적인 차이를 보이지 않았다. 혈액생화학 성분 중 total cholesterol 함량은 카테킨 처리에서 낮게 나타났으나(P<0.05), 비타민 C와 상호작용은 보이지 않았다. 혈액 내 SOD 활성은 카테킨 처리구에서 높았으며, corticosterone과 IgM 수준은 낮게 나타났으나(P<0.05), 비타민 C와 상호작용은 보이지 않았다. 간 내 스트레스 지표로 HSP70과 MDA는 비타민 C와 카테킨의 첨가에 따라 감소하였으며(P<0.05), HSP70에서 상호작용을 보였다(P<0.05). 가슴육 내 DPPH 라디칼 소거능은 카테킨을 급여한 처리에서 증가하였다(P<0.05). 고온스트레스에 대한 행동지표인 호흡수는 카테킨 처리에서 감소하는것으로 나타났으나(P<0.05), 비타민 C와의 상호작용은 보이지 않았다. 결론적으로 고온 환경에서 육계의 카테킨과 비타민 C의 첨가는 HSP70, MDA와 같은 스트레스 지표를 완화시켰으나, 첨가물질 간 상호작용과 최적의 혼합 수준에 대해 추가 연구가 요구된다.

Abstract ▼ AI-Helper

The study was carried out to investigate the effects of dietary combined supplementation of antioxidants as catechin and vitamin C on growth performance, meat quality, blood profiles and stress responses of broilers exposed to high temperature. For this experiment, a total of 360 21-day-old male Ross 308 broilers were used. Treatments were assigned with 6 replicates per treatment and 10 birds per replicate in a 2 × 3 factorial design with vitamin C (0, 250 mg/kg) and catechin (0, 600, 1,200 mg/kg). The heat stress environment was maintained at temperature 32±1℃ and relative humidity 60±5% for 24 hours until the end of the experiment. The supplemented antioxidants had no significant difference in weight gain, feed intake and feed conversion ratio (P>0.05). The content of total cholesterol in blood had no interaction, but decrease (P<0.01) in the supplemented catechin group. Also, the supplementation with catechin showed increase in the SOD activity of blood, and lower corticosterone and IgM levels of broilers. The contents of HSP70 and MDA in liver decrease (P<0.05) with the supplementation of antioxidants, and HSP70 showed an interaction between groups. DPPH radical scavenging ability in breast meat increased (P<0.01) in catechin, but meat quality did not show difference according to treatments. Respiratory rate decreased (P<0.05) in catechin, but no interaction with vitamin C. In conclusion, the combination of vitamin C and catechin can alleviate stress under high temperature, such as HSP70 and MDA, but further study on the optimal supplemental level is needed.

주제어

표/그림 (8)

표 Table 1. Effect of combined supplementation of catechin and vitamin C supplementation on growth performance in broilers during high temperature
표 Table 2. Effect of combined supplementation of catechin and vitamin C supplementation on meat quality of broilers during high temperature
표 Table 3. Effect of combined supplementation of catechin and vitamin C supplementation on composition of leukocyte and erythrocyte profile in broilers during high temperature
표 Table 4. Effect of combined supplementation of catechin and vitamin C supplementation on biochemical profiles of blood in broilers during high temperature
표 Table 5. Effect of combined supplementation of catechin and vitamin C supplementation on stress index in blood of broilers during high temperature
표 Table 6. Effect of combined supplementation of catechin and vitamin C supplementation on stress index in liver of broilers during high temperature
표 Table 7. Effect of combined supplementation of catechin and vitamin C supplementation on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of breast meat of broilers during high temperature
표 Table 8. Effect of combined supplementation of catechin and vitamin C supplementation on rectal temperature (℃) and respiration rate (breaths/min) of broilers during high temperature (days 21-35)

AI 본문요약
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문제 정의

따라서, 본 연구는 고온 환경에서 육계에 비타민 C(0, 250 mg)와 카테킨(0, 600, 1,200 mg/kg)을 급이 시 생산성, 혈액 성상 및 스트레스 반응에 미치는 영향을 구명하고자 수행하였다.

제안 방법

사료 내 첨가제 급여에 대한 항산화수준 분석을 위해 혈청 내 SOD(Superoxide dismutase) 활성은 SOD assay kit WST(Dojindo, Tokyo, Japan)를 이용하여 분석하였다. 스트레스호르몬 분석은 corticosterone ELSIA kit(ADI-900-097, Enzo Life Sciences, Inc.
4를 이용하였으며, 2 × 3 요인 실험에 따른 분산분석을 통하여 조사항목에서의 비타민 C 첨가 유무 및 카테킨의 첨가 수준, 그리고 각 물질 간 상호작용에 대한 유의성을 검정하였다. 설계한 각 반복은 성장에 대한 매개변수(체중, 증체량, 사료섭취량 및 사료 요구율) 분석을 위한 실험 단위이며, 각각의 선별 개체는 계육 품질, 혈액 및 간 조직 분석, 직장 온도와 호흡수 분석을 위한 단위로 사용하였다. 처리구간의 95% 수준에서 유의성이 인정된 조사항목에 대하여 Duncan’s multiple range test를 이용하여 평균값 간의 유의성을 검정하였다.
시험 종료일에 처리당 반복별로 2수씩 평균 체중과 유사한 개체를 선별하여 혈액, 가슴육 및 간을 채취하였다. 혈액은 익하정맥에서 5 mL씩 채취하여 EDTA가 처리된 tube와 serum separator tube(BD Biosciences, Franklin Lakes, NJ, USA)에 나누어 담았다.
처리구는 후기사료에 비타민 C(0, 250 mg/kg)와 카테킨(0, 600, 1,200 mg/kg)의 단독 및 혼합 급여에 따른 2×3 요인시험으로 설계하였으며, 무첨가구, 비타민 C 250 mg/kg, 카테킨 600 mg/kg, 카테킨 1,200 mg/kg, 비타민 C 250 mg/kg+카테킨 600 mg/kg, 비타민 C 250 mg/kg+카테킨 1,200 mg/kg으로 구분하여 처리 당 6반복, 반복 당 10수씩 완전 임의 배치하였다. 시험사료는 옥수수-대두박 위주의 시판사료를 전기(CP 20.05%, ME 3,100 kcal/kg) 및 후기(CP 19.0%, ME 3,150 kcal/kg)를 이용하였으며, 각 처리별 사료는 시판사료에 각각의 첨가제를 혼합하여 급여하였다. 본 시험에 사용한 첨가물은 녹차에서 추출한 카테킨 분말(Red Star Pharmaceutical Co.
처리구는 후기사료에 비타민 C(0, 250 mg/kg)와 카테킨(0, 600, 1,200 mg/kg)의 단독 및 혼합 급여에 따른 2×3 요인시험으로 설계하였으며, 무첨가구, 비타민 C 250 mg/kg, 카테킨 600 mg/kg, 카테킨 1,200 mg/kg, 비타민 C 250 mg/kg+카테킨 600 mg/kg, 비타민 C 250 mg/kg+카테킨 1,200 mg/kg으로 구분하여 처리 당 6반복, 반복 당 10수씩 완전 임의 배치하였다

대상 데이터

공시동물은 21일령 수컷 Ross 308 품종 육계 360수를 공시하였으며, 총 2주간 시험을 실시하였다. 처리구는 후기사료에 비타민 C(0, 250 mg/kg)와 카테킨(0, 600, 1,200 mg/kg)의 단독 및 혼합 급여에 따른 2×3 요인시험으로 설계하였으며, 무첨가구, 비타민 C 250 mg/kg, 카테킨 600 mg/kg, 카테킨 1,200 mg/kg, 비타민 C 250 mg/kg+카테킨 600 mg/kg, 비타민 C 250 mg/kg+카테킨 1,200 mg/kg으로 구분하여 처리 당 6반복, 반복 당 10수씩 완전 임의 배치하였다.
0%, ME 3,150 kcal/kg)를 이용하였으며, 각 처리별 사료는 시판사료에 각각의 첨가제를 혼합하여 급여하였다. 본 시험에 사용한 첨가물은 녹차에서 추출한 카테킨 분말(Red Star Pharmaceutical Co., Ltd, Anhui, China)과 상업용 비타민 C 분말(L-ascorbic acid, CSPC Pharmaceutical Group Ltd, Shijiazhuang, China)을 사용하였다.

데이터처리

본 시험에서 얻어진 모든 자료들의 통계분석은 SAS(Statistics analytical system) 9.4를 이용하였으며, 2 × 3 요인 실험에 따른 분산분석을 통하여 조사항목에서의 비타민 C 첨가 유무 및 카테킨의 첨가 수준, 그리고 각 물질 간 상호작용에 대한 유의성을 검정하였다
처리구간의 95% 수준에서 유의성이 인정된 조사항목에 대하여 Duncan’s multiple range test를 이용하여 평균값 간의 유의성을 검정하였다.

이론/모형

가슴육 내 DPPH 라디칼 소거능을 측정하기 위해 Blois(1958)의 방법을 이용하여 측정하였다. 메탄올에 용해시킨 0.
지질과산화도는 thiobarbituric acid 방법에 따라 생성된 malondialdehyde(MDA) 함량을 532nm 흡광도에서 측정하였다. 간 조직의 단백질 분석은 BCA방법(Rierce BCA protein, Assay Kit 23225, Thermo Scientific, MA, USA)을 이용해 분석하였으며, MDA 함량은 단백질 g당 농도로 나누어 표시하였다.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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