[논문]월비가출탕(越婢加朮湯)의 항산화 및 항염증 효과에 대한 연구

선윤권; 최진봉; 임승규; 설재욱; 김선종; 정재민

doi:10.18325/jkmr.2023.33.1.1

월비가출탕(越婢加朮湯)의 항산화 및 항염증 효과에 대한 연구
The Study on Anti-Oxidant and Anti-Inflammatory Effects of Wolbigachul-tang 원문보기

Journal of Korean Medicine Rehabilitation : JKMR = 한방재활의학과학회지, v.33 no.1, 2023년, pp.1 - 12

선윤권 (동신대학교 한의과대학 침구학교실) , 최진봉 (동신대학교 한의과대학 한방재활의학교실) , 임승규 (동신대학교 한의과대학 한방재활의학교실) , 설재욱 (동신대학교 한의과대학 한방재활의학교실) , 김선종 (동신대학교 한의과대학 한방재활의학교실) , 정재민 (동신대학교 한의과대학 한방재활의학교실)

Abstract ▼ AI-Helper

Objectives This study was conducted to find out the antioxidant and inflammatory improvement effects of Wolbigachul-tang. Methods To evaluate the antioxidant and anti-inflammatory effects of wolbigachul-tang, the total polyphenol and flavonoid contents were measured. And radical scavenging function was measured. In addition reactive oxygen species, nitric oxide, cytokines productions were measured by treating wolbigachul-tang in the lipopolysaccharide-induced RAW264.7 cells. We also measured the gene expression of inducible nitric oxide synthase, cyclooxygenase-2, cytokines too. Results Radical scavenging experiments showed significant concentration-dependent scavenging capacity increase. The amount of reactive oxygen species, nitric oxide, cytokines production in the cell all showed a significant concentration-dependent decrease compared to the control group. The gene expression of inducible nitric oxide synthase, cyclooxygenase-2, and cytokines also showed a significant concentration-dependent decrease compared to the control group. Conclusions In conclusion, it is believed that wolbigachul-tang can be applied to various inflammatory diseases.

주제어

표/그림 (16)

표 Table I. The Prescription of Wolbigachul-tang
그림 Fig. 1. DPPH radical scavenging activity of WGT. The results were expressed as mean±standard error of mean from three independent experiments. DPPH: 2,2-diphenyl-2-picryl-hydrazyl, WGT: wolbigachul-tang.
그림 Fig. 2. ABTS radical scavenging activity of WGT. The results were expressed as mean±standard error of mean from three independent experiments. ABTS: 2,2'-azinobis-(3-ethylbenzothiazoline-6- sulfonic acid), WGT: wolbigachul-tang.
표 Table II. Real-Time PCR Primer Sequences
그림 Fig. 3. Cell viability of WGT in RAW264.7 cells. The result were presented by the mean±standard error of mean from three independent experiments. WGT: wolbigachul-tang, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400, 800: lipopolysaccharides-induced and WGT (100, 200, 400, 800 μg/mL) treated RAW264.7 cells.
그림 Fig. 4. Effect of WGT on reactive oxygen species level in RAW264.7 cells. The result were presented by the mean±standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, *p<0.05, **p< 0.01, ***p<0.001 compared to control). WGT: wolbigachul-tang, Normal: non lipopolysaccharides-induced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 5. Effect of WGT on nitric oxide level in RAW264.7 cells. The result were presented by the mean±standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, **p<0.01, ***p< 0.001 compared to control). WGT: wolbigachul-tang, Normal: non lipopolysaccharides-induced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 6. Effect of WGT on prostaglandin E2 level in RAW264.7 cells. The result were presented by the mean±standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, *p<0.05, ***p< 0.001 compared to control). WGT: wolbigachul-tang, Normal: non lipopolysaccharides-induced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 7. Effect of WGT on interleukin 1 beta level in RAW264.7 cells. The result were presented by the mean±standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, *p<0.05, ***p< 0.001 compared to control). WGT: wolbigachul-tang, Normal: non lipopolysaccharides-induced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 8. Effect of WGT on interleukin 6 level in RAW264.7 cells. The result were presented by the mean±standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, ***p<0.001 compared to control). WGT: wolbigachul-tang, Normal: non lipopolysaccharidesinduced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 9. Effect of WGT on tumor necrosis factor α level in RAW264.7 cells. The result were presented by the mean± standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, **p<0.01, ***p<0.001 compared to control). WGT: wolbigachultang, Normal: non lipopolysaccharides-induced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 11. Effect of WGT on COX-2 mRNA expression level in RAW264.7 cells. The result were presented by the mean± standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, **p<0.01, ***p<0.001 compared to control). WGT: wolbigachultang, COX-2: cyclooxygenase-2, Normal: non lipopolysaccharidesinduced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 10. Effect of WGT on iNOS mRNA expression level in RAW264.7 cells. The result were presented by the mean± standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, *p< 0.05, **p<0.01, ***p<0.001 compared to control). WGT: wolbigachul-tang, iNOS: inducible nitric oxide synthase, Normal: non lipopolysaccharides-induced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 12. Effect of WGT on IL-1β mRNA expression level in RAW264.7 cells. The result were presented by the mean±standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, *p<0.05, ***p< 0.001 compared to control). WGT: wolbigachul-tang, IL-1β: interleukin-1β, Normal: non lipopolysaccharides-induced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 13. Effect of WGT on IL-6 mRNA expression level in RAW264.7 cells. The result were presented by the mean± standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, *p< 0.05, ***p<0.001 compared to control). WGT: wolbigachul-tang, IL-6: interleukin-6, Normal: non lipopolysaccharides-induced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.
그림 Fig. 14. Effect of WGT on TNF-α mRNA expression level in RAW264.7 cells. The result were presented by the mean± standard error of mean from three independent experiments (significance of results: +++p<0.001 compared to normal, ***p<0.001 compared to control). WGT: wolbigachul-tang, TNF-α: tumor necrosis factor α, Normal: non lipopolysaccharidesinduced RAW264.7 cells, Control: lipopolysaccharides-induced RAW264.7 cells, 100, 200, 400: lipopolysaccharides-induced and WGT (100, 200, 400 μg/mL) treated RAW264.7 cells.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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