[논문]병풀(Centella asiatica) 착즙액과 추출물의 Asiaticoside 분석법 검증 및 항산화 활성

김연숙; 신현영; 하은지; 구자평; 정세빈; 김가을에; 정미연; 유광원

doi:10.9799/ksfan.2023.36.2.093

병풀(Centella asiatica) 착즙액과 추출물의 Asiaticoside 분석법 검증 및 항산화 활성
Validation of Asiaticoside as Marker Compound of Centella asiatica Juice and Extract, and Its Antioxidant Activity 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.2, 2023년, pp.93 - 102

김연숙 (한국교통대학교 식품영양학전공) , 신현영 (고려대학교 대학원 의생명융합과학과 러닝헬스시스템 융합전공) , 하은지 (한국교통대학교 식품영양학전공) , 구자평 (한국교통대학교 식품영양학전공) , 정세빈 (한국교통대학교 식품영양학전공) , 김가을에 (한국교통대학교 식품영양학전공) , 정미연 ((주)참선진녹즙) , 유광원 (한국교통대학교 식품영양학전공)

Abstract ▼ AI-Helper

Centella asiatica (C. asiatica) has been widely used in food, cosmetics, and pharmaceutical industry as a functional material. In a previous study, we have investigated not only pharmacological effects such as antioxidative and anti-inflammatory effects, but also analyzed various functional ingredients. In this study, triterpenoids were analyzed using HPLC-DAD to determine marker compounds among functional ingredients. When triterpenoids were analyzed, asiaticoside from C. asiatica was determined as an optimal marker compound. Next, specificity, linearity, limited of detection (LOD), limited of quantification (LOQ), precision, accuracy, and range were evaluated using HPLC-DAD to determine asiaticoside contents in C. asiatica juice and extracts. The specificity was elucidated by chromatogram and retention time using an established analytical method. The coefficient of correlation obtained was 0.9996. LOD was 4.99 ㎍/mL and LOQ was 15.12 ㎍/mL. Intra- and inter-day precision of asiaticoside were determined to be 0.48~1.68% and 0.08~1.09%, respectively. Furthermore, the recovery rate of asiaticoside was 98.88% and the analytical range of Field-70E was determined to be 0.625~10 mg/mL. As a results of evaluating ABTS, DPPH, and FRAP antioxidative effect, Field-70E showed potent antioxidant activities. Results of this study could be used as basic data for quality standardization of C. astiatica juice and extracts.

주제어

표/그림 (10)

그림 Fig. 1. Chemical structure of (A) madecassoside, (B) asiaticoside, (C) madecassic acid, and (D) asiatic acid.
표 Table 1. HPLC conditions for the quantitative analysis of triterpenoids
그림 Fig. 2. HPLC chromatogram of (A) Field-70E and (B) standard mixture.
표 Table 2. Contents of major triterpenoids in C. asiatica juice and extracts
그림 Fig. 3. HPLC chromatogram of asiaticoside and blank.
표 Table 3. Linearity regression, limited of detection (LOD), and limited of quantification (LOQ) for asiaticoside
표 Table 4. Precision of intra- and inter-day variabilities for asiaticoside
그림 Fig. 4. Contents of asiaticoside in C. asiatica juice and extract. All results of composition content are expressed as mean±S.D. for three independent experiments. Bars with different letters are significantly different (p<0.05) according to Duncan’s multiple range test. HE: hot-water extracts, 70E: 70% EtOH extracts.
그림 Fig. 5. Antioxidant activity of C. asiatica juice and extract. (A) ABTS and DPPH radical scavenging effect and (B) FRAP activity. All results of composition content are expressed as mean±S.D. for three independent experiments. Bars with different letters are significantly different (p<0.05) according to Duncan's multiple range test. HE: hot-water extracts, 70E: 70% EtOH extracts.
표 Table 5. Recovery rate of analytical method for asiaticoside

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