[논문]하수슬러지 처리에서 미생물과 메탄올 적용을 통한 암모니아 배출 감소 및 식물 성장 향상 연구

김진원; 양희건; 양희종; 류명선; 하광수; 정수지; 이수영; 서지원; 정도연

doi:10.13087/kosert.2023.26.2.13

하수슬러지 처리에서 미생물과 메탄올 적용을 통한 암모니아 배출 감소 및 식물 성장 향상 연구
Reducing Ammonia Emissions and Enhancing Plant Growth through Co-application of Microbes and Methanol in Sewage Sludge Treatment 원문보기

環境復元綠化 = Journal of the Korean Society of Environmental Restoration Technology, v.26 no.2, 2023년, pp.13 - 24

김진원 ((재)발효미생물산업진흥원) , 양희건 ((재)발효미생물산업진흥원) , 양희종 ((재)발효미생물산업진흥원) , 류명선 ((재)발효미생물산업진흥원) , 하광수 ((재)발효미생물산업진흥원) , 정수지 ((재)발효미생물산업진흥원) , 이수영 ((재)발효미생물산업진흥원) , 서지원 ((재)발효미생물산업진흥원) , 정도연 ((재)발효미생물산업진흥원)

Abstract ▼ AI-Helper

Sewage sludge has been widely used as an organic fertilizer in agriculture. However, sewage sludge can cause serious malodor problems resulting from the decomposition of organic compounds in anaerobic conditions. The malodor of sewage sludge mainly occurs due to a low carbon to nitrogen ratio (C/N), high moisture, and low temperature, which are ideal conditions for ammonia emissions. Therefore, in this study, we investigated the reduction of the odor-causing ammonia nitrogen (NH3-N) in sewage sludge by co-application of microbes and methanol (MeOH). The physico-chemical properties of the municipal sewage sludge showed that the odor was mainly caused by a higher NH3-N content (2932.2 mg L-1). Supplementation with MeOH (20%) as a carbon source in the sewage sludge significantly reduced the NH3-N up to 34.2% by increasing C/N ratio. Furthermore, the sewage sludge was treated with the NH3-N reducing and plant growth promoting (PGP) bacteria Stenotrophomonas rhizophila SRCM 116907. The treatment with S. rhizophila SRCM 116907 significantly increased the seedling vigor index of Lolium perenne (10.3%) and Chrysanthemum burbankii (42.4%). The findings demonstrate that supplementing sewage sludge with methanol significantly reduces ammonia emissions, thereby mitigating malodor problems. Overall, the study highlights the potential of using a microbial and methanol approach to improve the quality of sewage sludge as an organic fertilizer and promote sustainable agriculture.

주제어

표/그림 (9)

그림 Figure 1. The graphical abstract of the sewage sludge composting process adding MeOH and PGPB for reducingmalodor.
표 Table 1. Chemical properties of the experiment raw sewage sludge (SS) and sewage sludge containing soil (CS)
그림 Figure 2. Influence of initial methanol concentration on the NH3-N removal efficiency in the sewage sludge (p < 0.05, n = 3).
그림 Figure 3. Growth curves (A) and NH₃-N concentration change (B) of selected microbes in the M9 minimal medium containing 1000 mg NH₃-N L^-1.
표 Table 2. Extracellular enzymes activities and plant growth promoting properties analysis of selected microbes
표 Table 3. Number of sequences, alpha-diversity of different sewage sludge treatments
그림 Figure 4. The growth of the Lolium perenne (A), Chrysanthemum burbankii (B), it's seedling vigor index (C, D), and plant growth monitoring image (E, F). Bars represent standard deviations and different letters are indicating significant differences among the treatments according to the Duncan's multiple range test (p < 0.05, n = 10).
그림 Figure 5. Stacked histogram (A) and heatmap (B) illustrate the bacterial relative abundance of taxa at the species level in the sewage sludge samples.
표 Table 4. 16S rRNA reads analysis result in genus level at different treatments

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