[논문]익모초, 어성초, 진피 복합 추출물의 항염증 및 항산화 평가

이훈규; 지중구

doi:10.12925/jkocs.2023.40.1.35

익모초, 어성초, 진피 복합 추출물의 항염증 및 항산화 평가
Evaluation of Anti-inflammatory and Antioxidant Abilities of Complex Extracts Produced from Leonurus japonicus Houtt., Houttuynia cordata Thunberg, and Citrus unshiu Markovich 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.40 no.1, 2023년, pp.35 - 47

이훈규 (중부대학교 대학원 한약자원학과) , 지중구 (중부대학교 한방보건제약학과)

초록
AI-Helper

본 연구는 복합추출물(익모초, 어성초, 진피)의 생리활성을 측정하여 식·의약품, 화장품 등의 소재로서의 가능성을 확인하고자 하였다. 복합추출물은 열수 추출하였으며, 세포독성, 항산화, 항염증, MAPKs 등의 평가는 ELISA 및 western blot을 통해 확인하였다. 그 결과, 총 폴리페놀 함량은 35.53±0.48 mg GAE/g, 플라보노이드 함량은 8.18±0.16 mg QE/g으로 나타났으며, DPPH 와 ABTS radical 소거능은 농도 의존적으로 증가하였다. 대조군과 비교하여 세포독성은 400 ㎍/㎖ 농도까지 100% 이상의 생존율이 나타났으며, NO 및 염증성 사이토카인을 유의하게 감소시켰다. 또한, 복합추출물은 MAPKs(ERK, JNK, p38) 단백질 발현을 유의하게 억제시켰다. 이러한 결과는 복합추출물이 산화적 손상 및 염증에 사용될 수 있음을 나타낸다. 따라서 본 연구 결과는 식·의약품, 화장품용 복합소재의의 기초자료로 활용될 수 있을 것이다.

Abstract ▼ AI-Helper

This study aimed to confirm the potential as a material for food, medicine, and cosmetics by measuring the physiological activity of the complex extract(Leonurus japonicus Houtt., Houttuynia cordata Thunberg and Citrus unshiu Markovich). The complex extract was with hot water, The evaluation of cytotoxicity, antioxidant, anti-inflammatory, and MAPKs of the extract was performed by using ELISA and western blot. Total polyphenols and flavonoid contents of complex extracts were 126.16 mg/g and 105.84 mg/g, respectively. The scavenging activities of DPPH and ABTS radical significantly increased in a dose-dependent in complex extracts. Compared to the control group, the complex extracts treatments significantly reduced concentrations of nitric oxide and inflammatory cytokine (i.e., IL-1β, TNF-α, and IL-6). Furthermore, the complex extracts treatments significantly reduced protein expression levels of MAPKs (i.e., ERK, JNK, and p38). The results indicate that the complex extract can be used for oxidative damage and inflammation. Thus, the results of this study can be used as basic data for composite materials for food, medicine, and cosmetics.

주제어

표/그림 (8)

표 Table 1. Total Polyphenol Contents of LHC extracts
그림 Fig. 1. DPPH radical scavenging activity of LHC extracts. LHC extracts were incubated at 1000, 100, 10, and 1 ㎍/ ㎖ with DPPH solution for 30 min. Activities were determined by measurement of absorbance at 517 ㎚. The results were expressed as mean ± S.D from three independent experiments.
그림 Fig. 2. ABTS radical scavenging activity of LHC extracts. LHC extracts were incubated at 1000, 100, 10, and 1 ㎍/ ㎖ with ABTS solution for 10 min. Activities were determined by measurement of absorbance at 732 ㎚. The results were expressed as mean ± S.D from three independent experiments.
표 Table 2. Total Flavonoid Contents of LHC extracts
그림 Fig. 3. Cell viability of LHC extracts in RAW 264.7 cell. RAW 264.7 cell were treated by 25, 50, 100, 200, 400, and 800 ㎍/㎖ of LHC extracts for 24 h. Treated cells were reacted by EZ-Cytox for 30 min and then absorbance were measured at 450 ㎚ using micro plate reader. Cell viability were calculated as percentage relative to the control. The result were presented by the mean ± S.D from three independent experiments.
그림 Fig. 4. Effect of LHC extracts on Nitric oxide level in RAW 264.7 cell. RAW 264.7 cell were treated 50, 100, 200 and 400 ㎍/㎖ of LHC extracts with 100 ng/㎖ LPS for 24 h. Nitric oxide levels were calculated as percentage relative to the control. The result were presented by the mean ± S.D from three independent experiments (Significance of results, ^*** : p＜0.001 compared to control).
그림 Fig. 5. Effect of LHC extracts on IL-1β(A), IL-6(B), TNF-α(C) level in RAW 264.7 cell. RAW 264.7 cell were treated 50, 100, 200, and 400 ㎍/㎖ of LHC extracts with 100 ng/㎖ LPS for 24 h. Treated cells were measured by mouse IL-1beta, IL-6, TNF-α ELISA kit. The result were presented by the mean ± S.D from three independent experiments (Significance of results, ^* : p＜0.05 ^** : p＜0.01, ^*** : p＜0.001 compared to control).
그림 Fig. 6. Effect of LHC extracts on ERK(A), JNK(B), and p38(C) protein expression level in RAW 264.7 cell. RAW 264.7 cell were treated 50, 100, 200, and 400 ㎍/㎖ of LHC extracts with 100 ng/㎖ LPS for 24 h. The total cell extracts were subjected to 10% SDS-PAGE and western blot analysis with the respective primary and secondary antibodies. The result were presented by the mean ± S.D from three independent experiments(Significance of results, ^** : p＜0.01, ^*** : p＜0.001 compared to control).

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저자의 다른 논문 :

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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