[논문]어성초의 항혈전, 항당뇨 및 항산화 활성

김종식; 이윤진; 강덕경; 이만효; 손호용

doi:10.5352/jls.2023.33.1.43

어성초의 항혈전, 항당뇨 및 항산화 활성
Antithrombosis, Antidiabetes, and Antioxidant Activities of Houttuynia cordata 원문보기

생명과학회지 = Journal of life science, v.33 no.1, 2023년, pp.43 - 49

김종식 (안동대학교 생명과학부) , 이윤진 (안동대학교 식품영양학과) , 강덕경 (안동대학교 식품영양학과) , 이만효 (경북바이오산업연구원) , 손호용 (안동대학교 식품영양학과)

초록
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어성초(Houttuynia cordata)는 삼백초과(Saururacease)에 속하는 다년생 초본으로 잎, 줄기 및 뿌리의 전초를 약제로 사용하고 있으며, 민간에서는 혈행개선, 폐렴, 급만성기관지염, 장염, 요로감염증, 종기 치료에 주로 이용되고 있다. 최근에는 항산화, 항염증, 항균 및 암세포 성장억제, 간손상 및 신경세포 사멸 억제, 카드뮴 독성 감소효과 등이 보고되고 있다. 본 연구에서는 어성초 지상부의 에탄올 추출물 및 이의 헥센 분획물, 에틸아세테이트 분획물, 부탄올 분획물 및 물 잔류물을 조제하여 항혈전, 항당뇨, 항산화 및 적혈구용혈활성을 평가하였다. 어성초의 총 폴리페놀 및 총 플라보노이드 함량은 에틸아세테이트 분획물에서 가장 높았으며, 상기 분획물은 5 mg/ml 농도에서 용매 대조구보다 2.09배, 2.19배 및 1.69배 연장된 TT, PT, aPTT를 보여 강력한 항혈전 활성을 나타내었으며, DPPH, ABTS 및 nitrite radical에 대해 각각 30.9, 19.9 및 49.6 ㎍/ml의 RC50을 보여, 9.8, 5.2 및 11,8 ㎍/ml의 RC₅₀를 나타낸 vitamin C의 항산화력에 비교되었다. 트롬빈 저해활성이 강력한 헥센 분획물이 강력한 적혈구 용혈활성을 나타내는 바와 달리, 상기 에틸아세테이트 분획물은 1 mg/ml 농도까지 전혀 용혈활성이 나타나지 않았다. 본 연구결과는 어성초의 항혈전 활성에 대한 최초의 보고로, 어성초의 quercitirin, hyperoside, orientin 및 isoquercitrin이 항혈전 및 항산화 활성에 관련되어 제시하고 있으며, 향후 어성초를 이용한 기능성 항혈전 식품소재 개발 가능함을 제시하고 있다.

Abstract ▼ AI-Helper

Houttuynia cordata belongs to the Saururacease family and its leaves, stems, and roots have been used as oriental medicines to treat pneumonia, acute or chronic bronchitis, enteritis, and abscesses and to remove extravasated blood. Recently, the antioxidant, anti-inflammation, antibacterial, and anti-proliferation activities and protection abilities of H. cordata against liver and neuron cell damage have been reported. In this study, ethanol extract and its solvent fractions (fractions of hexane, ethyl acetate, butanol, and water residue) were prepared, and their antithrombosis, antidiabetes, antioxidant, and hemolysis activities were evaluated. The ethyl-acetate fraction of H. cordata (EF-HC) showed the highest polyphenol and flavonoids contents among the fractions and exhibited strong antithrombosis and antioxidant activities. The EF-HC at 5 mg/ml showed 2.09-folds of thrombin time, 2.19-folds of prothrombin time, and 1.69-folds of activated partial thromboplastin time compared to the their solvent control and 30.9, 19.9, and 49.6 ㎍/ml of RC50 against DPPH, ABTS, and nitrite radicals, respectively. Furthermore, the EF-HC did not show any hemolytic activity up to 1 mg/ml, whereas the hexane fraction of H. cordata showed 55% hemolysis at 1 mg/ml. This is the first report of the antithrombosis activity of H. cordata. Our results suggest that quercitirin, hyperoside, orientin, and isoquercitrin in EF-HC are related to its antithrombosis and antioxidant activities and that the EF-HC could be developed as a promising antithrombosis agent.

주제어

표/그림 (6)

$Table 1. Contents of total polyphenol, total flavonoid, total sugar and reducing sugar of the ethanol extract of Houttyunia cordata and subsequent organic solvent fractions$ 표 Table 1. Contents of total polyphenol, total flavonoid, total sugar and reducing sugar of the ethanol extract of Houttyunia cordata and subsequent organic solvent fractions
$Table 2. Antioxidant activities of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions$ 표 Table 2. Antioxidant activities of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions
$Fig. 1. Anti-thrombosis activity of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions. Symbols; EE: ethanol extract of Houttyunia cordata (HC), HF-HC: Hexane fraction of the HC extract, EF-HC: Ethylacetate fraction of the HC extract, BF-HC: Butanol fraction of the HC extract and WR: water residue fraction of the HC extract. Data are presented as relative clotting time based on solvent control (x control). The thrombin time (TT), prothrombin time (PT) and activated partial thromboplastin time (aPTT) of solvent control (dimethylsulfoximide) were 24.5 sec, 16.8 sec and 40.1 sec, respectively. Different letters within a panel differ significantly (p<0.05).$ 그림 Fig. 1. Anti-thrombosis activity of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions. Symbols; EE: ethanol extract of Houttyunia cordata (HC), HF-HC: Hexane fraction of the HC extract, EF-HC: Ethylacetate fraction of the HC extract, BF-HC: Butanol fraction of the HC extract and WR: water residue fraction of the HC extract. Data are presented as relative clotting time based on solvent control (x control). The thrombin time (TT), prothrombin time (PT) and activated partial thromboplastin time (aPTT) of solvent control (dimethylsulfoximide) were 24.5 sec, 16.8 sec and 40.1 sec, respectively. Different letters within a panel differ significantly (p<0.05).
$Fig. 2. Inhibitory activities against α-glucosidase of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions. Symbols; EE: ethanol extract of Houttyunia cordata (HC), HF-HC: Hexane fraction of the HC extract, EF-HC: Ethylacetate fraction of the HC extract, BF-HC: Butanol fraction of the HC extract and WR: water residue fraction of the HC extract. Different letters within a panel differ significantly (p< 0.05).$ 그림 Fig. 2. Inhibitory activities against α-glucosidase of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions. Symbols; EE: ethanol extract of Houttyunia cordata (HC), HF-HC: Hexane fraction of the HC extract, EF-HC: Ethylacetate fraction of the HC extract, BF-HC: Butanol fraction of the HC extract and WR: water residue fraction of the HC extract. Different letters within a panel differ significantly (p< 0.05).
$Table 3. Calculated RC<sub>50</sub>s of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions$ 표 Table 3. Calculated RC₅₀s of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions
$Table 4. Hemolytic of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions against human red blood cells$ 표 Table 4. Hemolytic of the ethanol extract of Houttyunia cordata and its subsequent organic solvent fractions against human red blood cells

AI 본문요약
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대상 데이터

본 실험의 어성초는 2021년 전남에서 수확된 지상부를 안동 약업사로부터 구입하여 사용하였으며 시료는 안동대학교 식품영양학과에서 보관하고 있다(voucher specimen 2022-HC1). Dimethyl sulfoxide (DMSO), Triton X-100 및 기타 사용 시약은 Sigma Co.

데이터처리

분석결과는 SPSS 27.0 버전을 사용하여 mean±SD로 나타내었으며, 각 군 간의 차이는 ANOVA로 분석하였으며 유의수준은 p<0.05로 하였다.

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