[논문]편백 오일의 항주름, 피부 장벽 및 보습능 평가

강은정; 장영아; 이진태; 김성희; 김소현; 박지아; 최윤식

doi:10.12925/jkocs.2023.40.2.309

편백 오일의 항주름, 피부 장벽 및 보습능 평가
The effects of Chamaecyparis obtusa oil on anti-wrinkle, skin-barrier and moisturizing 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.40 no.2, 2023년, pp.309 - 321

강은정 (경성대학교 약학과) , 장영아 (경성대학교 스마트헬스케어융복합연구센터) , 이진태 (경성대학교 화장품학과) , 김성희 (경성대학교 화장품학과) , 김소현 (경성대학교 화장품학과) , 박지아 (경성대학교학교 제약공학과) , 최윤식 (경성대학교 약학과)

초록
AI-Helper

편백나무(Chamaecyparis obtusa)는 측백나무과에 속하며 우리나라 남쪽 지역에서 주로 잘 자생한다. 편백나무의 목재는 재질이 우수하여 가구로서의 활용이 높으며 남은 목재, 가지, 잎은 정유 추출에 사용되고 있다. 편백 정유는 항염, 향균, 탈취, 진정 효능이 탁월한 것으로 알려져 있다. 본 연구는 이러한 편백나무의 잎에서 추출한 오일이 주름 개선, 피부 장벽 및 보습능에 미치는 영향을 평가하고자 하였다. 먼저, DPPH assay와 ABTS assay를 통해 항산화 활성을 평가한 결과, 편백 오일은 두 가지 라디칼을 농도 의존적으로 유의하게 소거하였다. 그리고 주름 생성 억제 효능 평가를 위해 elastase 활성을 측정한 결과, 편백 오일은 elastase 활성을 직접적으로 억제시킴을 확인하였다. 다음으로는 real-time PCR을 통해 유전자 발현을 확인한 결과, 편백 오일은 인간섬유아세포에서 MMP-1 유전자 발현을 유의하게 감소시켰다. 또한, 편백 오일에 의해 각질형성세포에서 filaggrin과 HAS-2 유전자 발현이 유의하게 증가함을 확인하였다. 이러한 결과를 종합해보면, 편백 오일은 주름 개선과 피부 장벽 및 보습능 강화에 도움을 주어 항노화 화장품의 기능성 원료로서 활용될 수 있을 것으로 기대된다.

Abstract ▼ AI-Helper

Chamaecyparis obtusa (C. obtusa) belongs to Cupressaceae and grows wild in southern area of Korea. C. obtusa has been used for furnitures because of the superior quality of the lumber and remaining branches and leaves were used for extraction of essential oil (C. obtusa oil). C. obtusa oil has numerous health effects, including anti-inflammation, antibacterial, deodorization, sedative effect. This study was performed to evaluate the effects of C. obtusa oil on anti-wrinkles, skin-barrier and moisturizing. First, DPPH and ABTS assay were conducted to evaluate antioxidant effect and C. obtusa oil showed significance scavenging activity on both radicals. And elastase activity was examined for anti-wrinkle effect. At the result, elastase activity was significantly reduced by C. obtusa oil. Next, real-time PCR was performed to evaluate gene expression. At the results, MMP-1 mRNA expression was significantly reduced by C. obtusa oil in HDF cells. In addition, filaggrin and HAS-2 mRNA expression significantly increased by C. obtusa oil in HaCaT cells. Taken together, these results indicate that C. obtusa oil has potential as a cosmetic material for anti-aging by enhancing anti-wrinkle, skin-barrier and moisturizing effects.

주제어

표/그림 (9)

표 Table 1. Primer sequence
표 Table 2. Analysis of GC-MS
그림 Fig. 1. DPPH radical scavenging effect of C. obtusa oil. DPPH radical significantly decreased by C. obtusa oil does- dependently. Data are presented as mean ± SEM. ^***p< 0.001 compared to control, ^###p<0.001 compared to A.A. A.A; ascorbic acid.
그림 Fig. 2. ABTS radical scavenging effect of C. obtusa oil. ABTS⁺ radical significantly decreased by C. obtusa oil dose- dependently. Data are expressed as mean ± SEM. ^***p< 0.001 compared to control. A.A; ascorbic acid.
그림 Fig. 3. Effects of C. obtusa oil on elastase activity. Elastase activity dose- dependently decreased by C. obtusa oil. Data are presented as mean ± SEM. ^***p< 0.001 compared to control, ^###p<0.001 compared to EGCG. EGCG; (-)-Epigallocatechin Gallate.
그림 Fig. 4. Cell viability of C. obtusa oil. Cell viability decreased by SFE C. obutsa oil in HDF cell (A) and HaCaT cell (B). Cell viability was recovered by mixture (SFE+SDE) of C. obutsa oil in HDF cell (C) and HaCaT cell (D). Data are expressed as mean ± SEM. ^***p< 0.001 compared to control. SFE; supercritical fluid extraction, SDE; steam dilution extraction.
그림 Fig. 5. Anti-wrinkle effect of C. obtusa oil. The expression of MMP-1 mRNA decreased by SFE, SDE and mixture of C. obtusa oil (SFE+SDE). Data are presented as mean ± SEM. ^*p< 0.05, ^***p< 0.001 compared to control.
그림 Fig. 6. Effect of C. obtusa oil on Skin-barrier protection. The expression of filaggrin mRNA significantly increased by SFE and mixture of C. obtusa oil (SFE+SDE). Data are presented as mean ± SEM. ^***p< 0.001 compared to control.
그림 Fig. 7. Effect of C. obtusa oil on skin moisturizing. The expression of HAS-2 mRNA significantly increased by SFE and mixture of C. obtusa oil (SFE+SDE). Data are expressed as mean ± SEM. ^***p< 0.001 compared to control.

참고문헌 (36)

H. B. Kang, Y. Choi, J. Bak, Y. Jang, "Effect of Lysimachia christinae Hance extract on moisturizing functionand protection of skin barrier", The Korean Society of Culture and Convergence, Vol.42, No.12 pp. 941-958, (2020).？
S. K. Choi, K. D. Park, D. A. Kim, D. W. Lee, Y. J. Kim, "Preparation of Camel Milk Liposome and Its Anti-Aging Effects", Journal of the Society of Cosmetic Scientists of Korea, Vol.40, No.2 pp. 155-162, (2014).？
H. Y. Yu, I. J. Yang, V. Lincha, I. S. Park, D. Lee, H. M. Shin, "The Effects of the fruits of Foeniculum vulgare no Skin Barrier Function and Hyaluronic Acid Production in HaCaT Keratinocytes", Journal of Life Science, Vol.25, No.8 pp. 880-888, (2015).？
H. Wang, K. Lee, Y. Kang, "Skin Brrier Improvement Effect of Exosomal Nanovesicles Derived from Lactic acid Bacteria", Journal of the Society of Cosmetic Scientists of Korea, Vol.47, No.2 pp. 171-178, (2021).？
S. Park, M. Beak, J. Yang, "Skin Anti-aging Moisturizing, and Barrier Protective Effects of Plectranthus Tomentosa: A Comparative Study of Water and Ethanol Extracts", Journal of the Korean Society of Cosmetology, Vol.25, No.5 pp. 1061-1067, (2019).？
S. Hyung, K. Min, Y. Kim, "Alleviating Effect of Gardeniae fructus Water Extract on Inflammation and Skin-Barrier Damage", The Journal of Cosmetological Science, Vol.6, No.1 pp.63-72, (2010).？
J. Necas, L. Bartosikova, P. Brauner, J. Kolar, "Hyaluronic acid(hyaluronan): a review", Veterinarni Medicina, Vol.53, No.5 pp. 397-411, (2008).？
E. Papakonstantinou, M. Roth, G. Karakiulakis, "Hyaluronic acid A key molecule in skin aging", Dermato-Enadocrinology Vol.4, No.3 pp. 253-258, (2012).？
Y. Kim, Y, Yoon, "Regulation of Col1A1 and MMP1 Expression by Taurine, Major Component of Oyster, in Human Dermal Fibroblasts", Korea Journal of Aesthetics and Cosmetology Vol.11, No.2 pp. 393-397, (2013).？
I. H. Joo, H. J. Choi, B. Y. Sim, G. Y. Min, D. H. Kim, "Effects of Cuscuta Chinensis Lamark Ethanol Extract on Wrinkle Improvement Bio-markers by UVB-induced CCD-986Sk Cell", Journal of Physiology & Pathology in Korean Medicine Vol.35, No.5 pp.321-327, (2018).？
J. Lee, D. Kim, E. Kim, H. J. Kwon, B. W. Kim, J. Cho, H. Kim, T. H. Kim, "Matrix metalloproteinase-1 suppression and type-1 procollagen synthesis promoting effects of Uncaria gambir", The Korean Society of Food Preservation Vol.24, No.1 pp. 93-99, (2017).？
B. K. Kim, J. H. Kang, G. H. Oh, J. Hwang, S. O. Jang, M. Kim, "Antibacterial and Antioxidant Activity of Chamaecyparis obsuta Extracts", Journal of Life Science Vol.29, No.7 pp.785-791, (2019).？
Y. Joung, Y. Kim, Y. Jang, "Studies on the Antioxidant and Whitening Effects of Chamaecyparis obtusa Extract", Journal of the Korean Applied Science and Technology Vol.37, No.6 pp. 1496-1506, (2020).？
J. K. Yang, B. K. Kang, T. H. Kim, S. C. Hong, W. T. Seo, M. S. Choi, "Efficient Extraction Methods and Analysis of Essential Oil from Softwood Leaves", Korean Society for Biotechnology and Bioengineering Journal Vol.14, No.4 pp. 357-364, (2002).？
G. Lim, R. Kim, H. Cho, Y. Moon, C. Choi, "Comparison of Volatile Compounds of Chamaecyparis obtusa Essential Oil and its Application on the Improvement of Atopic Dermatitis", Korean Society for Biotechnology and Bioengineering Journal Vol.28, No.2 pp. 115-122, (2013).？
H. S. Kim, S. K. Han, J. Y. Mang, "Evaluations on the Deodorization Effect and Antibacterial Activity of Chamaecyparis obsuta Essential Oil", Korean Jounal of Odor Research and Engineering Vol.8, No.3 pp. 101-107, (2009).？
Y. Jo, "Optimization of supercritical fluid extraction of phytoncide from Chamaecyparis obtusa leaf", master's thesis Department of Industrial Engineering, Chonnam National University, (2014).？
J. Kim, W. Choi, S. Lee, M. Park, "Comparative Study on The Composition of Essential Oil by Supercritical Carbon Dioxide Extraction and Hydro-distillation from Chamaecyparis obtusa Leaves", Journal of The Korean Wood Science and Technology Vol.43, No.4 pp. 494-503, (2015).？
B. M. Kwak, E. Kim, Y. Kim, H. Kim, "Component analysis of four-part extracts from Chamaecyparis obsuta Endl. by supercritical fluid extraction and anti-inflammatory effect on RAW 264.7cells", Journal of Exercise Rehabilitation Vol.15, No.5 pp. 723-730, (2019).？
M. Lee, S. Park, S. Lee, H. Lee, D. Kil, "Emission Characteristics of Volatile Organic Compounds by Humidifier with Using Hinoki Cyperss Extracts", Journal of The Korean Wood Science and Technology Vol.42, No.6 pp. 747-757, (2014).？
J. Choi, J. Park, Y. Cho, "Development of Functional Products Using Chamaecyparis Obtusa Heartwood Essential Oil", Journal of Convergence for Information Technology Vol.11, No.2 pp. 211-218, (2021).？
J. H. Kim, S. Lee, K. B. Do, W. D. Ji, S. G. Kim, Y. D. Back, K. Kim, "Analysis of the Component and Immunological Efficacy of Chamaecyparis obsuta Leaf Extract", The Korean Journal of Clinical Laboratory Science Vol.50, No.1 pp. 37-43, (2018).？
M. A. Jang, J. M. Lee, "Research on domestic and inernational industrial trends of functional cosmetics", Journal of the Korean Applied Science and Technology Vol.38, No.2 pp. 618-627, (2021).？
J. Bak, S. J. Lee, T. W. Kim, S. Hwang, M. J. Park, R. Aruanchalam, E. Yoo, M. H. Park, Y. Choi, H. K. Kim, "Schisandrol A and gomisin N from Schisandra chinensis extract improve hypogonadism via anti-oxidative stress in TM3 Leydig cells", Nutrition Research and Practice Vol.17, No.1 pp. 1-12, (2022).？
Y. Jang, J. Lee, "The Evaluation of Antioxidant, Anti-inflammatory, and Anti-aging of Extract Solvent and Poria cocos by Parts", Korea Journal of Aesthetics and Cosmetology Vol.13, No.3 pp. 373-383, (2015).？
A. Vimal, D. Pal, T. Tripathi, A. Kumar, "Eucalyptol, sabinene and cinnamaldehyde: potent inhibitors of salmonella target protein L-asparaginase", Springer-Verlag GmbH Germany 3 Biotech Vol.7, No.4 pp. 258, (2017).？
P. R. Quiroga, C. M. Asensio, V. Nepote, "Antioxidant effects of the monoterpenes carvacrol, thymol and sabinene hydrate on chemical and sensory stability of roasted sunflower seeds", Society of Chemical Industry Vol.95, pp. 471-479, (2013).？
J. Valente, M. Zuzarte, M. J. Goncalves, M. C. Lopes, C. Cavaleiro, L. Salgueiro, M. T. Ctuz, "Antifungal, antioxidant and anti-inflammatory activities of Oenanthe crocata L. essential oil", Food and Chemical Toxicology Vol.62, pp. 349-354, (2013).？

상세보기
M. J. Kim, J. H. Kim, S. Lee, E. J. Cho, H. Y. Kim, "Determination of Radical Scavenging Activity of Aster yomena (Kitam.) Honda", Journal of the Korea Academia-Industrial cooperation Society Vol.19, No.9 pp. 402-407, (2018).？
Y. Jang, S. Park, B. Kim, J. Park, Y. Jeong, J. Lee, "Effect of ethanol extract of Lotus Rhizome and node of Lotus Rhizome", Journal of Oil & Applied Science Vol.34, No.3 pp. 657-665, (2017).？
A. Floegel, D. Kim, S. Chung, S. I. Koo, O. K. Chun, "Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods", Journal of Food Composition and Analysis Vol.24, pp. 1043-1048, (2011).？

상세보기
Y. Jang, S. Kim, H. Kim, J. Lee, "Biological Activity and Component Analyses of Chamaecyparis obtusa Leaf Extract: Evaluation of Antiwrinkle and Cell Protection Effects in UVA-Irradiated Cells", Medicina Vol.59, No.4 pp. 755, (2023).？
H. Koo, C. Ha, S. Kim, S. Jang, H. Lim, Y. Kim, E. Sohn, "Evaluation of Whitening, Anti-wrinkle, and Moisturizing Effects of Phragmitis rhizoma", Journal of the Korea Academia-Industrial Vol.22, No.11 pp. 277-286, (2021).？
J. Lee, S. Hwang, T. Shen, J. H. Kim, L. You, W. Hu, J. Y. Cho, "Enhancement of skin barrier and hydration-related molecules by protopanaxatriol in human keratinocytes", Journal of Ginseng Research Vol.45, pp. 354-360, (2021).？

원문보기 상세보기
M. Han, J. Bae, J. Ban, H. S. Shin, D. H. Lee, J. H. Chung, "Black rice (Oryza sativa L.) extract modulates ultraviolet-induced expression of matrix metalloproteinases and procollagen in a skin cell model", International Journal of Molecular Medicine Vol.41, pp. 3073-3070, (2018).？

상세보기
S. Yang, C. Lee, S. Y. Cha, J. Choi, S. Lee, "Skin Barrier Enhancement of Ferment using Lava Seawater and Lactobasillus plantarum HDB1234 as a Novel Cosmetic Ingredient", Journal of the Korean Society of Cosmetology Vol.27, No.2 pp. 356-363, (2021).

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증