[논문]용아초(龍芽草) 유기용매 분획물의 항산화 및 피부 장벽 개선 효과

한제근; 김민주; 박해진; 이경하; 노성수

doi:10.6116/kjh.2023.38.3.19

용아초(龍芽草) 유기용매 분획물의 항산화 및 피부 장벽 개선 효과
Anti-oxidant Activity and Skin Barrier Function Measured in Different Solvent Fractions Obtained from Agrimoniae Herba 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.38 no.3, 2023년, pp.19 - 26

한제근 ((주)원일바이오) , 김민주 (대구한의대학교 간질환한약융복합활용연구센터) , 박해진 (대구한의대학교 바이오융복합시험센터) , 이경하 (부산대학교 분자생물학과) , 노성수 (대구한의대학교 한의학과 본초약리학교실)

Abstract ▼ AI-Helper

Objectives : Agrimoniae Herba is a herbal medicine widely distributed in Asia and contains flavonoids including catechin, quercitrin, rutin, hyperoside, and quercetin. This study aimed to investigate the anti-oxidant activity and skin barrier function of different solvent fractions (Hexane; methylene chloride, MC; ethyl acetate, EA; n-butanol, Bu; Water) obtained from Agrimoniae Herba. Methods : Anti-oxidant activity of different solvent fractions obtained from Agrimoniae Herba was investigated through total polyphenol contents, total flavonoid contents, 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity measurements. Then, filament aggregating protein (Filaggrin), Type I collagen, ceramide synthase (CERS) 3, and CERS4 were analyzed to evaluate the skin barrier strengthening effect of different solvent fractions obtained from Agrimoniae Herba on UVB-stimulated HaCaT cells. Results : As a result of measuring total polyphenol contents, total flavonoid contents, DPPH free radical scavenging activity, and ABTS radical scavenging activity, antioxidant activity was found to be excellent in the order of EA > Bu > MC > Hexane > Water. As a result of measuring mRNA gene expression of Type I collagen, Filaggrin, CERS3, and CERS4 after UVB-stimulated was applied to HaCaT cells treated with different solvent fractions obtained from Agrimoniae Herba, it was found to increase significantly in the Bu-treated group. Conclusion : Our findings show that the Bu sample obtained from Agrimoniae Herba has excellent anti-oxidant ability, which increases Type I collagen, Filaggrin, and ceramide synthetase in UVB-stimulated HaCaT cells to control the skin barrier improvement effect.

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표/그림 (9)

표 Table 1. Primer sequences used for PCR analysis
표 Table 2. Total polyphenol content
표 Table 3. Total flavonoid content
표 Table 4. DPPH free radical scavenging activity
표 Table 5. ABTS radical scavenging activity
$Figure 1. Cells viability effects of solvent fractions obtained from a Agrimoniae Herba extract in HaCaT cells. All data are expressed mean±SEM (n=3). Significance: ***p<0.001 vs. DMSO group by t-test.$ 그림 Figure 1. Cells viability effects of solvent fractions obtained from a Agrimoniae Herba extract in HaCaT cells. All data are expressed mean±SEM (n=3). Significance: ***p<0.001 vs. DMSO group by t-test.
$Figure 2. Effects of solvent fractions obtained from a Agrimoniae Herba extract on the Type I procollagen and Filaggrin levels in HaCaT cells. All data are expressed mean±SEM (n=3). Significance: *p<0.05 vs. UVB group by t-test.$ 그림 Figure 2. Effects of solvent fractions obtained from a Agrimoniae Herba extract on the Type I procollagen and Filaggrin levels in HaCaT cells. All data are expressed mean±SEM (n=3). Significance: *p<0.05 vs. UVB group by t-test.
$Figure 3. Effect of solvent fractions obtained from a Agrimoniae Herba extract on mRNA expression of Type I collagen and filaggrin in HaCaT cells. All data are expressed mean±SEM (n=3). Significance: *p<0.05 vs. UVB group by t-test.$ 그림 Figure 3. Effect of solvent fractions obtained from a Agrimoniae Herba extract on mRNA expression of Type I collagen and filaggrin in HaCaT cells. All data are expressed mean±SEM (n=3). Significance: *p<0.05 vs. UVB group by t-test.
$Figure 4. Effect of solvent fractions obtained from a Agrimoniae Herba extract on mRNA expression of CERS3 and CERS4 in HaCaT cells. All data are expressed mean±SEM (n=3). Significance: *p<0.05 and **p<0.01 vs. UVB group by t-test.$ 그림 Figure 4. Effect of solvent fractions obtained from a Agrimoniae Herba extract on mRNA expression of CERS3 and CERS4 in HaCaT cells. All data are expressed mean±SEM (n=3). Significance: *p<0.05 and **p<0.01 vs. UVB group by t-test.

참고문헌 (31)

Choi JH, Hwang SJ, Jeong SN, Lee YK, Jin MH,？Park SG, Lee CK. Anti-aging Effect on Skin with 9？Repetitive Steaming and Fermenting Process Herbal？Composition Extract. J. Herbology 2009;24(4):101-6.
Kim HS. Effect of Nepeta cataria extract on the skin？barrier function. Korean J. Food Preserv. 2020;27(2):242-6. doi:org/10.11002/kjfp.2020.27.2.242.

상세보기
Chaquour B, Seite S, Coutant K, Fourtanier A,？Borel JP, Bellon G. Chronic UVB- and all-trans？retinoic-acid-induced qualitative and quantitative？changes in hairless mouse skin. J Photochem？Photobiol B. 1995;28(2):125-35. doi:10.1016/1011-1344(94)07080-8.

상세보기
Jeon HY, Kim JK, Kim WG, Lee SJ. Beneficial Effect？of Curcumin on Epidermal Permeability Barrier？Function in Hairless Rat. KOREAN J. FOOD SCI.？TECHNOL. 2008;40(6):686-90.
Park CS. Skin barrier and beauty foods. Food Sci.？2008;40:19-26. doi:org/10.23093/FSI.2007.40.4.19.

원문보기 상세보기
Choi YS, Kim YS. Neuroprotective Effects of？Agrimoniae Herba against Intrastriatal Hemorrhage？in Rats. Kor. J. Herbology 2010;25(4):31-7.
Xu X, Qi X, Wang W, Chen G. Separation and？determination of flavonoids in Agrimonia pilosa Ledeb.？by capillary electrophoresis with electrochemical？detection. J Sep Sci. 2005;28(7):647-52. doi:10.1002/jssc.200400095.

상세보기
Pan Y, Liu HX, Zhuang YL, Ding LQ, Chen LX, Qiu F.？[Studies on isolation and identification of flavonoids？in herbs of Agrimonia pilosa]. Zhongguo Zhong Yao？Za Zhi. 2008;33(24):2925-8.

상세보기
Kumar A, Sehgal N, Kumar P, Padi SS, Naidu PS.？Protective effect of quercetin against ICV colchicine-induced cognitive dysfunctions and oxidative？damage in rats. Phytother Res. 2008;22(12):1563-9. doi: 10.1002/ptr.2454.

상세보기
Rezai-Zadeh K, Ehrhart J, Bai Y, Sanberg PR,？Bickford P, Tan J, Shytle RD. Apigenin and luteolin？modulate microglial activation via inhibition of？STAT1-induced CD40 expression. J？Neuroinflammation. 2008;5:41. doi:10.1186/1742-2094-5-41.

상세보기
Yoon JY, Lee SY, Jun HJ, Lee JY. Anti-aging Effects？of Solvent Fraction from Agrimonia pilosa L. Extracts？[Internet]. Vol. 55, Journal of Applied Biological？Chemistry. J Appl Biol Chem 2012;55(1):35-9.？doi:org/10.3839/jabc.2011.056.

원문보기 상세보기
Kim HJ, Lim HW, Kim BH, Choi SW, Yoon CS.？Studies on the Anti-acne Effect of Agrimonia pilosa？Ledeb. J Soc Cosmet Scientists Korea 2006;32(1):53-8.
Kang SC, Lee CM, Koo HJ, Ahn DH, Choi H, Lee？JH, Bak JP, Lee MH, Choung ES, Kawk JH.？Hepatoprotective effects of aqueous extract from？aerial part of Agrimmony. Kor J Pharmacogn.？2006;37(1):28-32.
Hua CL, Lee JK, Cho KH, Kang DG, Kwon TO, Kwon JW, Kim JS, Sohn EJ, Lee HS. Mechanism
for the vascular relaxation induced by butanol？extract of Agrimonia pilosa. Kor J Pharmacogn.？2006;37(2):67-73.
Choi SJ, Baik JW, Park JH, Jun CY, Choi YK, Ko？SG. Mechanism of apoptosis & tumor growth？inhibition of Agrimonia pilosa Ladebour (APL) in？vitro and in vivo. Korean J Orient Int Med.？2009;30(2):399-409.
Kim JJ, Jiang J, Shim DW, Kwon SC, Kim TJ, Ye？SK, Kim MK, Shin YK, Koppula S, Kang TB, Choi？DK, Lee KH. Anti-inflammatory and anti-allergic？effects of Agrimonia pilosa Ledeb extract on murine？cell lines and OVA-induced airway inflammation. J？Ethnopharmacol. 2012;140(2):213-21. doi:10.1016/j.jep.2011.12.035.

상세보기
Saeed N, Khan MR, Shabbir M. Antioxidant？activity, total phenolic and total flavonoid contents？of whole plant extracts Torilis leptophylla L. BMC？Complement Altern Med. 2012;12:221. doi:10.1186/1472-6882-12-221.

상세보기
Malla MY, Sharma M, Saxena RC, Mir MI, Mir AH,？Bhat SH. Phytochemical screening and spectroscopic？determination of total phenolic and flavonoid？contents of Eclipta Alba Linn. J. Nat. Prod. Plant？Resour. 2013;3(2):86-91.
Blosis MS. Antioxidant determinations by the use of a stable free radical. Nature. 1958;26:1199-200.？doi:org/10.1038/1811199a0.

상세보기
Re R, Pellegrini N, Proteggente A, Pannala A, Yang？M, Rice-Evans C. Antioxidant activity applying an？improved ABTS radical cation decolorization assay.？Free Radic Biol Med. 1999;26(9-10): 1231-7. doi:10.1016/s0891-5849(98)00315-3.

상세보기
Ha CW, Sohn EH, Kim SH, Jang S, Park MR, Kim？YK, Bae IY. Fermented Angelicae tenussimae with？Aspergillus oryzae Improves Skin Barrier Properties,？Moisturizing, and Anti-Inflammatory Responses.？Int J Mol Sci. 2022;23(20):12072. doi:10.3390/ijms232012072.

상세보기
Park NJ, Jo BG, Bong SK, Park SA, Lee S, Kim？YK, Yang MH, Kim SN. Lobelia chinensis Extract？and Its Active Compound, Diosmetin, Improve Atopic？Dermatitis by Reinforcing Skin Barrier Function？through SPINK5/LEKTI Regulation. Int J Mol Sci.？2022;23(15):8687. doi: 10.3390/ijms23158687.

상세보기
Koo JS. A Study on the Skin Improvement Effect？of Castanea crenata Inner Shell with Microneedle？Therapy System. Kor. J. Herbol. 2022;37(2):23-31.
Jing C, Guo J, Li Z, Xu X, Wang J, Zhai L, Liu J,？Sun G, Wang F, Xu Y, Li Z, Zhao D, Jiang R, Sun？L. Screening and Research on Skin Barrier Damage？Protective Efficacy of Different Mannosylerythritol？Lipids. Molecules. 2022;27(14):4648. doi:10.3390/molecules27144648.

상세보기
Baek J, Lee MG. Oxidative stress and antioxidant？strategies in dermatology. Redox Rep. 2016;21(4):164-9. doi:10.1179/1351000215Y.0000000015.

상세보기
Yoon YM, Yoon JY, Lim KM, Hahn HJ, Kim YR,？Ahn KJ, An SK. Effects of the Complex Containing？Centella asiatica - and Folic Acid-Ferment？Extracts, Acetyl Glutamine, and Nicotinic Acid？Adenine Dinucleotide Phosphate on the Inhibition？of Senescence and Melanogenesis, Promotion of？Collagen Expression, Cellular Regeneration, and？Keratinocyte Differentiation, and Anti-inflammation.？Kor. J. Aesthet. Cosmetol. 2013;11(4): 675-84.
Sandilands A, Sutherland C, Irvine AD, McLean？WH. Filaggrin in the frontline: role in skin barrier？function and disease. J Cell Sci. 2009;122(Pt？9):1285-94. doi:10.1242/jcs.033969.

상세보기
Coderch L, Lopez O, de la Maza A, Parra JL.？Ceramides and skin function. Am J Clin Dermatol.？2003;4(2):107-29. doi:10.2165/00128071-200304020-00004.

상세보기
Schurer NY, Plewig G, Elias PM. Stratum corneum？lipid function. Dermatologica. 1991;183(2):77-94.？doi: 10.1159/000247644.

상세보기
Jungersted JM, Hellgren LI, Jemec GB, Agner T.？Lipids and skin barrier function--a clinical？perspective. Contact Dermatitis. 2008;58(5):255-62. doi: 10.1111/j.1600-0536.2008.01320.x.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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