[논문]Noni Fruit Juice의 3T3-L1 지방전구세포 분화 억제 및 인터루킨-1β 유도 Inducible Nitric Oxide Synthase 염증유전자 발현 감소 효과

장병철

doi:10.15429/jkomor.2023.23.1.1

[국내논문] Noni Fruit Juice의 3T3-L1 지방전구세포 분화 억제 및 인터루킨-1β 유도 Inducible Nitric Oxide Synthase 염증유전자 발현 감소 효과
Suppressive Effects on Lipid Accumulation and Expression of Interleukin-1β-Mediated Inducible Nitric Oxide Synthase in 3T3-L1 Preadipocytes by a Standardized Commercial Noni Fruit Juice 원문보기

한방비만학회지 = Journal of Korean Medicine for Obesity Research, v.23 no.1, 2023년, pp.1 - 9

Abstract ▼ AI-Helper

Objectives: Noni fruit juice (NFJ) is liquor extracted from Morinda citrifolia (noni) fruit and has been used as an herbal remedy in many countries. However, the NFJ's anti-adipogenic and anti-inflammatory effects on adipocytes are poorly understood. The purpose of this study was to explore the commercially standardized NFJ effects on lipid accumulation throughout 3T3-L1 preadipocytes differentiation and interleukin-1β (IL-1β)-mediated inducible nitric oxide synthase (iNOS) expression in 3T3-L1 preadipocytes. Methods: Cellular lipid accumulation and triglyceride (TG) content in differentiating 3T3-L1 preadipocytes were assessed subsequently via the Oil Red O staining and AdipoRed assay. MTS assay was used to examine NFJ cytotoxicity in (differentiating) 3T3-L1 preadipocytes. Immunoblotting and reverse transcriptase polymerase chain reaction analysis were used to measure the expression levels of target protein and mRNA in (differentiating) 3T3-L1 preadipocytes, respectively. Results: NFJ treatment at 150 μL/mL led to a substantial reduction of fat accumulation and TG content during 3T3-L1 adipogenesis with no discernable impact on the cell viability. Of note, while NFJ treatment (150 μL/mL) largely inhibited the CCAAT/enhancer-binding protein-α (C/EBP-α) and peroxisome proliferator-activated receptor-β (PPAR-β) protein expressions, it did not influence PPAR-γ in differentiating 3T3-L1 preadipocytes. Of interest, treatment with IL-1β at 20 ng/mL for 4 hours elicited in firm induction of iNOS mRNA expression in 3T3-L1 preadipocytes. However, NFJ treatment at 100 or 200 μL/mL greatly attenuated the IL-1β-induced iNOS mRNA expression in 3T3-L1 preadipocytes. Conclusions: NFJ has anti-adipogenic and anti-inflammatory effects on (differentiating) 3T3-L1 preadipocytes which are in part intervened via control of the expression of C/EBP-α, PPAR-β, and iNOS.

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표/그림 (4)

그림 Fig. 1. Effects of different inducers on lipid accumulation in differentiating 3T3-L1 preadipocytes. (A) To induce adipocyte differentiation, 3T3-L1 preadipocytes were grown in induction medium containing MDI, insulin, and fetal bovine serum (FBS) for 8 days. On day 8 (D8), the intracellular lipid droplets were evaluated by Oil Red O staining (upper panels in A). Phasecontrast cell images of the differentiation D8 were also taken (lower panels in A) (x100). (B) 3T3-L1 preadipocytes were induced to differentiate with an induction medium containing MDI, insulin, and FBS for 8 days. On day 8 (D8), intracellular triglyceride (TG) stores was assessed by AdipoRed assay.
그림 Fig. 2. Effects of noni fruit juice (NFJ) at different concentrations on lipid accumulation, triglyceride (TG) content, and cell viability in differentiating 3T3-L1 preadipocytes. (A, B) To induce adipocyte differentiation, 3T3-L1 preadipocytes were grown in induction medium containing MDI, insulin, and fetal bovine serum for 8 days with or without of NFJ at the selected concentrations. On day 8 (D8), cell viability was calculated using MTS assay (A) and the intracellular lipid accumulation was estimated by Oil Red O staining (B) (x100). Values are mean±standard error (SE) of data from three independent experiments with triplicate. (C) Measurement of TG content in 3T3-L1 adipocytes (D8) that were grown in the absence or presence of NFJ at the designated concentrations by AdipoRed assay. Each experiment was conducted independently in triplication. Results are mean±SE of three independent experiments.
그림 Fig. 3. Effects of noni fruit juice (NFJ) at 150 μL/mL on the expression of CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor (PPAR)-β, and PPAR- γ in differentiating 3T3-L1 preadipocytes. To induce adipocyte differentiation, 3T3-L1 preadipocytes were grown in induction medium containing MDI, insulin, and fetal bovine serum (FBS) for 8 days with or without of NFJ at 150 μL/mL, and harvested at day 2 (D2), D5, and D8, subsequently. Whole-cell lysates at the selected time point were collected and analyzed by Western blot analysis with relevant antibodies.
그림 Fig. 4. Effects of noni fruit juice (NFJ) on the interleukin (IL)-1 β-induced inducible nitric oxide synthase (iNOS) expression in 3T3-L1 preadipocytes. (A) 3T3-L1 cells were incubated without or with IL-1β in the absence or presence of NFJ at the designated doses for 4 hours. Total cellular RNA from the conditioned cells was extracted and analyzed by reverse transcriptase polymerase chain reaction for measurement of the mRNA expression levels of iNOS or actin. (B) 3T3-L1 cells were incubated without or with IL-1β in the absence or presence of NFJ at the designated doses for 4 hours, followed measurement of cell viability by a MTS assay. Values are mean±standard error of results from three independent experiments with triplicate.

참고문헌 (40)

Boutari C, Mantzoros CS. A 2022 update on the epidemiology of obesity and a call to action: as its twin COVID-19 pandemic appears to be receding, the obesity and dysmetabolism pandemic continues to rage on. Metabolism. 2022 ; 133 : 155217.

상세보기
Phelan SM, Burgess DJ, Yeazel MW, Hellerstedt WL, Griffin JM, van Ryn M. Impact of weight bias and stigma on quality of care and outcomes for patients with obesity. Obes Rev. 2015 ; 16(4) : 319-26.

상세보기
Longo M, Zatterale F, Naderi J, Parrillo L, Formisano P, Raciti GA, et al. Adipose tissue dysfunction as determinant of obesity-associated metabolic complications. Int J Mol Sci. 2019 ; 20(9) : 2358.
Liu F, He J, Wang H, Zhu D, Bi Y. Adipose morphology: a critical factor in regulation of human metabolic diseases and adipose tissue dysfunction. Obes Surg. 2020 ; 30(12) : 5086-100.
Reyes-Farias M, Fos-Domenech J, Serra D, Herrero L, Sanchez-Infantes D. White adipose tissue dysfunction in obesity and aging. Biochem Pharmacol. 2021 ; 192 : 114723.

상세보기
Ali AT, Hochfeld WE, Myburgh R, Pepper MS. Adipocyte and adipogenesis. Eur J Cell Biol. 2013 ; 92(6-7) : 229-36.
Jakab J, Miskic B, Miksic S, Juranic B, Cosic V, Schwarz D, et al. Adipogenesis as a potential anti-obesity target: a review of pharmacological treatment and natural products. Diabetes Metab Syndr Obes. 2021 ; 14 : 67-83.

상세보기
Tang P, Virtue S, Goie JYG, Png CW, Guo J, Li Y, et al. Regulation of adipogenic differentiation and adipose tissue inflammation by interferon regulatory factor 3. Cell Death Differ. 2021 ; 28 : 3022-35.

상세보기
Farmer SR. Transcriptional control of adipocyte formation. Cell Metab. 2006 ; 4( 4) : 263-73.
Bahmad HF, Daouk R, Azar J, Sapudom J, Teo JCM, Abou-Kheir W, et al. Modeling adipogenesis: current and future perspective. Cells. 2020 ; 9(10) : 2326.

상세보기
Matsusue K, Peters JM, Gonzalez FJ. PPARβ/δ potentiates PPARγ-stimulated adipocyte differentiation. FASEB J. 2004 ; 18(12) : 1477-9.
Sarjeant K, Stephens JM. Adipogenesis. Cold Spring Harb Perspect Biol. 2012 ; 4( 9) : a008417.
Becerril S, Rodriguez A, Catalan V, Mendez-Gimenez L, Ramirez B, Sainz N, et al. Targeted disruption of the iNOS gene improves adipose tissue inflammation and fibrosis in leptin-deficient ob/ob mice: role of tenascin C. Int J Obes (Lond). 2018 ; 42 : 1458-70.

상세보기
Noronha BT, Li JM, Wheatcroft SB, Shah AM, Kearney MT. Inducible nitric oxide synthase has divergent effects on vascular and metabolic function in obesity. Diabetes. 2005 ; 54(4) : 1082-9.

상세보기
Dallaire P, Bellmann K, Laplante M, Gelinas S, CentenoBaez C, Penfornis P, et al. Obese mice lacking inducible nitric oxide synthase are sensitized to the metabolic actions of peroxisome proliferator-activated receptor-γ agonism. Diabetes. 2008 ; 57(8) : 1999-2011.
Samoylenko V, Zhao J, Dunbar DC, Khan IA, Rushing JW, Muhammad I. New constituents from noni (Morinda citrifolia) fruit juice. J Agric Food Chem. 2006 ; 54(17) : 6398-402.

상세보기
Chan-Blanco Y, Vaillant F, Mercedes Perez A, Reynes M, Brillouet JM, Brat P. The noni fruit (Morinda citrifolia L.): a review of agricultural research, nutritional and therapeutic properties. J Food Comp Anal. 2006 ; 19(6-7) : 645-54.
Zhang WM, Wang W, Zhang JJ, Wang ZR, Wang Y, Hao WJ, et al. Antibacterial constituents of Hainan Morinda Citrifolia (Noni) leaves. J Food Sci. 2016 ; 81(5) : M1192-6.

상세보기
Huang HL, Ko CH, Yan YY, Wang CK. Antiadhesion and anti-inflammation effects of Noni (Morinda citrifolia) fruit extracts on AGS cells during Helicobacter pylori infection. J Agric Food Chem. 2014 ; 62(11) : 2374-83.

상세보기
Palu AK, Seifulla RD, West BJ. Morinda citrifolia L. (noni) improves athlete endurance: its mechanisms of action. J Med Plant Res. 2008 ; 2(7) : 154-8.
Lin YL, Chang YY, Yang DJ, Tzang BS, Chen YC. Beneficial effects of noni ( Morinda citrifolia L.) juice on livers of high-fat dietary hamsters. Food Chem. 2013 ; 140(1-2) : 31-8.
Lin YL, Chou CH, Yang DJ, Chen JW, Tzang BS, Chen YC. Hypolipidemic and antioxidative effects of noni (Morinda citrifolia L.) juice on high- fat/cholesterol-dietary hamsters. Plant Foods Hum Nutr. 2012 ; 67(3) : 294-302.

상세보기
Nerurkar PV, Nishioka A, Eck PO, Johns LM, Volper E, Nerurkar VR. Regulation of glucose metabolism via hepatic forkhead transcription factor 1 (FOXO1) by Morinda citrifolia (noni) in high-fat diet-induced obese mice. Br J Nutr. 2012 ; 108(2) : 218-28.

상세보기
Jang BC. The fruit juice of Morinda citrifolia (noni) downregulates HIF-1α protein expression through inhibition of PKB, ERK-1/2, JNK-1 and S6 in manganese-stimulated A549 human lung cancer cells. Int J Mol Med. 2012 ; 29( 3) : 499-504.
McGillicuddy FC, Chiquoine EH, Hinkle CC, Kim RJ, Shah R, Roche HM, et al. Interferon γ attenuates insulin signaling, lipid storage, and differentiation in human adipocytes via activation of the JAK/STAT pathway. J Biol Chem. 2009 ; 284(46) : 31936-44.
Ellulu MS, Patimah I, Khaza'ai H, Rahmat A, Abed Y. Obesity and inflammation: the linking mechanism and the complications. Arch Med Sci. 2017 ; 13(4) : 851-63.

상세보기
Palu AK, Kim AH, West BJ, Deng S, Jensen J, White L. The effects of Morinda citrifolia L. (noni) on the immune system: its molecular mechanisms of action. J Ethnopharmacol. 2007 ; 115(3) : 502-6.
Kang JH, Song KB. Antibacterial activity of the noni fruit extract against Listeria monocytogenes and its applicability as a natural sanitizer for the washing of fresh-cut produce. Food Microbiol. 2019 ; 84 : 103260.

상세보기
Ida SR, Rodolfo QC, Jorge RA, Irving XR, Luicita LR, Isaac AM, et al. Beneficial effects of Morinda Citrifolia Linn. (noni) leaf extract on obesity, dyslipidemia and adiponectinemia in rats with metabolic syndrome. Int J Pharm Sci Res. 2017 ; 8(2) : 2496-503.
Motshakeri M, Ghazali HM. Nutritional, phytochemical and commercial quality of noni fruit: a multi-beneficial gift from nature. Trends Food Sci Technol. 2015 ; 45(1) : 118-29.

상세보기
Ong KW, Hsu A, Tan BK. Anti-diabetic and anti-lipidemic effects of chlorogenic acid are mediated by AMPK activation. Biochem Pharmacol. 2013 ; 85(9) : 1341-51.

상세보기
Cao Z, Umek RM, McKnight SL. Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells. Genes Dev. 1991 ; 5(9) : 1538-52.

상세보기
Ma X, Wang D, Zhao W, Xu L. Deciphering the roles of PPARγ in adipocytes via dynamic change of transcription complex. Front Endocrinol. 2018 ; 9 : 473.
Lee JE, Schmidt H, Lai B, Ge K. Transcriptional and epigenomic regulation of adipogenesis. Mol Cell Biol. 2019 ; 39(11) : e00601-18.

상세보기
de Sa PM, Richard AJ, Hardy H, Stephens JM. Transcriptional regulation of adipogenesis. Compr Physiol. 2017 ; 7( 2) : 635-74.
Rosen ED, Walkey CJ, Puigserver P, Spiegelman BM. Transcriptional regulation of adipogenesis. Genes Dev. 2000 ; 14(11) : 1293-307.

상세보기
Li H, Kang JH, Han JM, Cho MH, Chung YJ, Park KH, et al. Anti-obesity effects of soy leaf via regulation of adipogenic transcription factors and fat oxidation in diet-induced obese mice and 3T3-L1 adipocytes. J Med Food. 2015 ; 18( 8) : 899-908.
Lin YL, Chang YY, Yang DJ, Tzang BS, Chen YC. Beneficial effects of noni (Morinda citrifolia L.) juice on livers of high-fat dietary hamsters. Food Chem. 2013 ; 140(1-2) : 31-8.
Liu JL, Zhang R, Liu YB, Hou Y, Wang LY, Wang C, et al. Literature research and discussion of Chinese medicinal properties of Morinda citrifolia. Zhongguo Zhong Yao Za Zhi. 2020 ; 45(5) : 984-90.
Nerurkar PV, Hwang PW, Saksa E. Anti-diabetic potential of noni: the yin and the yang. Molecules. 2015 ; 20( 10) : 17684-719.

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