[논문]Hesperidin과 Hesperetin의 간 손상 동물모델에서 산화적 스트레스에 대한 간 보호 효과

김지현; 이여; 김미숙; 조은주; 김현영; 최진상

doi:10.15429/jkomor.2022.22.1.1

[국내논문] Hesperidin과 Hesperetin의 간 손상 동물모델에서 산화적 스트레스에 대한 간 보호 효과
Hesperidin and Hesperetin Protect against Oxidative Stress on Hepatic Toxicity in Rats 원문보기

한방비만학회지 = Journal of Korean Medicine for Obesity Research, v.22 no.1, 2022년, pp.1 - 10

김지현 (경상국립대학교 식품영양학과) , 이여 (부산대학교 식품영양학과) , 김미숙 (경상국립대학교 식품과학과) , 조은주 (부산대학교 식품영양학과) , 김현영 (경상국립대학교 식품영양학과) , 최진상 (경상국립대학교 식품공학과)

Abstract ▼ AI-Helper

Objectives: To investigate the protective effect of hesperidin and hesperetin against oxidative stress in 2,2'-azobis (2-aminopropane) dihydrochloride (AAPH)-induced liver toxicity in rats. Methods: Hesperidin or hesperetin (200 mg/kg/day, respectively) was orally administered for 7 days once daily in rats. Subsequently, AAPH (50 mg/kg/day) was administered intraperitoneally. Lipid peroxidation, nitric oxide production, catalase activity, and protein expressions of nuclear factor-kappa B (NF-κB) and inducible nitric oxide synthase (iNOS) in the liver tissues were measured. Results: Administration of hesperidin and hesperetin significantly decreased serum aspartate transaminase and alanine transaminase levels in AAPH-induced oxidative stress liver tissues compared with control group. Lipid peroxidation and nitric oxide (NO) production were also significantly reduced by hesperidin and hesperetin in AAPH-induced oxidative stress liver tissues. In particular, lipid peroxidation levels of hesperetin-administered group significantly decreased to 5.02 nmole/mg protein in oxidative stress rats. Hesperidin and hesperetin significantly increased antioxidant activity, such as that of catalase. Furthermore, administration of hesperidin and hesperetin substantially down-regulated the expression of NF-κB and iNOS in liver tissues. Administration of hesperidin reduced NO levels and iNOS expression more than in the hesperetin-administered group. Conclusions: Administration of hesperidin and hesperetin led to a reduction in AAPH-induced liver toxicity by regulating oxidative stress.

Keyword

표/그림 (6)

그림 Fig. 1. Effects of hesperidin and hesperetin on serum ALT (A) and AST (B) levels in AAPH-induced rats. Values are mean±standard deviation. Different letters (a-c) are significantly different (P<0.05) among groups by Duncan's multiple range test. ALT: alanine aminotransferase, AST: aspartate aminotransferase, AAPH: 2,2'-azobis (2-aminopropane) dihydrochloride.
그림 Fig. 2. Effects of hesperidin and hesperetin on lipid peroxidation in liver of AAPH-induced rats. Values are mean±standard deviation. Different letters (a-d) are significantly different (P<0.05) among groups by Duncan's multiple range test. MDA: malondialdehyde, AAPH: 2,2'-azobis (2-aminopropane) dihydrochloride.
표 Table 1. Effects of Hesperidin and Hesperetin on Body and Liver Weights in AAPH-Induced Rats
그림 Fig. 3. Effects of hesperidin and hesperetin on NO production in liver of AAPH-induced rats. Values are mean±standard deviation. Different letters (a-c) are significantly different (P<0.05) among groups by Duncan's multiple range test. NO: nitric oxide, AAPH: 2,2'-azobis (2-aminopropane) dihydrochloride.
그림 Fig. 5. Effects of hesperidin and hesperetin on protein expressions of NF-κB (A) and iNOS (B) in liver of AAPH-induced rats. Values are mean±standard deviation. Different letters (a-d) are significantly different (P<0.05) among groups by Duncan's multiple range test. NF-κB: nuclear factor-kappa B, iNOS: inducible nitric oxide synthase, AAPH: 2,2'-azobis (2-aminopropane) dihydrochloride.
그림 Fig. 4. Effects of hesperidin and hesperetin on catalase activity in liver of AAPH-induced rats. Values are mean±standard deviation. Different letters (a-c) are significantly different (P<0.05) among groups by Duncan's multiple range test. AAPH: 2,2'-azobis (2- aminopropane) dihydrochloride.

참고문헌 (42)

Apel K, Hirt H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol. 2004 ; 55 : 373-99.

상세보기
Weidinger A, Kozlov AV. Biological activities of reactive oxygen and nitrogen species: oxidative stress versus signal transduction. Biomolecules. 2015 ; 5 : 472-84.

상세보기
Valko M, Leibfritz D, Moncol J, Cronin MT, Mazur M, Telser J. Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 2007 ; 39 : 44-84.
Li S, Tan HY, Wang N, Zhang ZJ, Lao L, Wong CW, et al. The role of oxidative stress and antioxidants in liver diseases. Int J Mol Sci. 2015 ; 16 : 26087-124.
Sanchez-Valle V, Chavez-Tapia NC, Uribe M, Mendez-Sanchez N. Role of oxidative stress and molecular changes in liver fibrosis: a review. Curr Med Chem. 2012 ; 19 : 4850-60.

상세보기
Sakaguchi S, Takahashi S, Sasaki T, Kumagai T, Nagata K. Progression of alcoholic and non-alcoholic steatohepatitis: Common metabolic aspects of innate immune system and oxidative stress. Drug Metab Pharmacokinet. 2011 ; 26 : 30-46.

상세보기
Li A N, Li S, Zhang YJ, Xu XR, Chen YM, Li HB. Resources and biological activities of natural polyphenols. Nutrients. 2014 ; 6 : 6020-47.

상세보기
Cho AR, Kim DG, Kang JR, Kang MJ, Shin JH. Antioxidant and liver protective activity of combined extracts of medicinal herbs. J Korean Soc Food Sci Nutr. 2019 ; 48 : 1195-204.
Lee SH, Lee MS. The study of physiological and antimicrobial activities on the Citrus Junos extracts with its textures and skin. Journal of the Korea Academia-Industrial cooperation Society. 2017 ; 18 : 67-74.
Ham I, Jung ED, Lee K, Lee JH, Bu Y, Kim H, et al. Analysis of the content of hesperidin and essential oils from the peels of various Citrus species. Kor J Herbology. 2008 ; 23 : 159-70.
Garg A, Garg S, Zaneveld LJ, Singla AK. Chemistry and pharmacology of the Citrus bioflavonoid hesperidin. Phytother Res. 2001 ; 15 : 655-69.
Roohbakhsh A, Parhiz H, Soltani F, Rezaee R, Iranshahi M. Molecular mechanisms behind the biological effects of hesperidin and hesperetin for the prevention of cancer and cardiovascular diseases. Life Sci. 2015 ; 124 : 64-74.
Parhiz H, Roohbakhsh A, Soltani F, Rezaee R, Iranshahi M. Antioxidant and anti-inflammatory properties of the citrus flavonoids hesperidin and hesperetin: an updated review of their molecular mechanisms and experimental models. Phytother Res. 2015 ; 29 : 323-31.
Roohbakhsh A, Parhiz H, Soltani F, Rezaee R, Iranshahi M. Neuropharmacological properties and pharmacokinetics of the citrus flavonoids hesperidin and hesperetin--a mini-review. Life Sci. 2014 ; 113 : 1-6.
Ferreira de Oliveira J, Santos C, Fernandes E. Therapeutic potential of hesperidin and its aglycone hesperetin: cell cycle regulation and apoptosis induction in cancer models. Phytomedicine. 2020 ; 73 : 152887.

상세보기
Dhanya R, Jayamurthy P. In vitro evaluation of antidiabetic potential of hesperidin and its aglycone hesperetin under oxidative stress in skeletal muscle cell line. Cell Biochem Funct. 2020 ; 38 : 419-27.

상세보기
Kim HY, Li L, Cho EJ. Antioxidative effects of hesperidin and hesperetin under in vitro. Cancer Prev Res. 2010; 15 : 333-9.
Cho EJ, Li L, Yamabe N, Kim HY. A ntioxidative effects of hesperidin and hesperetin under cellular system. Korean J Agric Sci. 2011; 38 : 717-22.
Wan J, Kuang G, Zhang L, Jiang R, Chen Y, He Z, et al. Hesperetin attenuated acetaminophen-induced hepatotoxicity by inhibiting hepatocyte necrosis and apoptosis, oxidative stress and inflammatory response via upregulation of heme oxygenase-1 expression. Int Immunopharmacol. 2020 ; 83 : 106435.

상세보기
Li S, Qin Q, Luo D, Pan W, Wei Y, Xu Y, et al. Hesperidin ameliorates liver ischemia/reperfusion injury via activation of the Akt pathway. Mol Med Rep. 2020 ; 22 : 4519-30.
Mihara M, Uchiyama M. Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem. 1978 ; 86 : 271-8.

상세보기
Hevel JM, Marletta MA. Nitric-oxide synthase assays. Meth Enzymol. 1994 ; 233 : 250-8.
Hugo A. Catalase in vitro. Meth Enzymol. 1984 ; 105 : 121-6.
Terao K, Niki E. Damage to biological tissues induced by radical initiator 2,2'-azobis(2-amidinopropane) dihydrochloride and its inhibition by chain-breaking antioxidants. J Free Radic Biol Med. 1986 ; 2 : 193-201.

상세보기
Han KH, Jeon YJ, Athukorala Y, Choi KD, Kim CJ, Cho JK, et al. A water extract of Artemisia capillaris prevents 2,2'-azobis(2-amidinopropane) dihydrochloride-induced liver damage in rats. J Med Food. 2006 ; 9 : 342-7.
Thapa BR, Walia A. Liver function tests and their interpretation. Indian J Pediatr. 2007 ; 74 : 663-71.

상세보기
Giannini E, Botta F, Fasoli A , Ceppa P, Risso D, Lantieri PB, et al. Progressive liver functional impairment is associated with an increase in AST/ALT ratio. Dig Dis Sci. 1999 ; 44 : 1249-53.

상세보기
Bartsch H, Nair J. Chronic inflammation and oxidative stress in the genesis and perpetuation of cancer: role of lipid peroxidation, DNA damage, and repair. Langenbecks Arch Surg. 2006 ; 391 : 499-510.
Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives in biological samples: Analytical and biological challenges. Anal Biochem. 2017 ; 524 : 13-30.

상세보기
Kim JH, Li L, Kim MS, Cho EJ, Kim HY. Protective effects of hesperidin and hesperetin on kidney toxicity by regulation of antioxidant activity in AAPH-induced rats. J Agri Life Sci. 2022 ; 56 : 111-6.
Galla HJ. Nitric oxide, NO, an intercellular messenger. Angew Chem Int Ed. 1993 ; 32 : 378-80.
Papi S, Ahmadizar F, Hasanvand A. The role of nitric oxide in inflammation and oxidative stress. Immunopathol Persa. 2019 ; 5 : e08.
Mates JM, Perez-Gomez C, De Castro IN. Antioxidant enzymes and human diseases. Clin Biochem. 1999 ; 32 : 595-603.

상세보기
Kaushal J, Mehandia S, Singh G, Raina A, Arya SK. Catalase enzyme: application in bioremediation and food industry. Biocatal Agric Biotechnol. 2018 ; 16 : 192-9.

상세보기
Zhang X, Yu Y, Lei H, Cai Y, Shen J, Zhu P, et al. The Nrf-2/HO-1 signaling axis: a ray of hope in cardiovascular diseases. Cardiol Res Pract. 2020 ; 2020 : 5695723.

상세보기
Dhanya R, Jayamurthy P. In vitro evaluation of antidiabetic potential of hesperidin and its aglycone hesperetin under oxidative stress in skeletal muscle cell line. Cell Biochem Funct. 2020 ; 38 : 419-27.

상세보기
Park HK, Kang SW, Park MS. Hesperidin ameliorates hepatic ischemia-reperfusion injury in sprague-dawley rats. Transplant Proc. 2019 ; 51 : 2828-32.

상세보기
Li J, Wang T, Liu P, Yang F, Wang W, Zheng W, et al. Hesperetin ameliorates hepatic oxidative stress and inflammation via the PI3K/AKT-Nrf2-ARE pathway in oleic acid-induced HepG2 cells and a rat model of high-fat diet-induced NAFLD. Food Funct. 2021 ; 12 : 3898-918.

상세보기
Heo SD, Kim J, Choi Y, Ekanayake P, Ahn M, Shin T. Hesperidin improves motor disability in rat spinal cord injury through anti-inflammatory and antioxidant mechanism via Nrf-2/HO-1 pathway. Neurosci Lett. 2020 ; 715 : 134619.

상세보기
Lawrence T. The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol. 2009 ; 1 : a001651.

상세보기
Cildir G, Low KC, Tergaonkar V. Noncanonical NF-κ B signaling in health and disease. Trends Mol Med. 2016 ; 22 : 414-29.
Cinelli MA, Do HT, Miley GP, Silverman RB. Inducible nitric oxide synthase: regulation, structure, and inhibition. Med Res Rev. 2020 ; 40 : 158-89.

상세보기

저자의 다른 논문 :

활용도 분석정보

상세보기

다운로드

내보내기

활용도 Top5 논문

해당 논문의 주제분야에서 활용도가 높은 상위 5개 콘텐츠를 보여줍니다.
더보기 버튼을 클릭하시면 더 많은 관련자료를 살펴볼 수 있습니다.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증