[논문]벚나무 추출물의 OVA 유도 천식동물모델에서 항염증 효능

김명규; 강순아

doi:10.9799/ksfan.2023.36.3.172

벚나무 추출물의 OVA 유도 천식동물모델에서 항염증 효능
Ant-Inflammatory Effect of Prunus serrulata var. spontanea Extract in OVA-Induced Asthma Animal Model 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.3, 2023년, pp.172 - 184

김명규 (호서대학교 보건산업연구소) , 강순아 (호서대학교 벤처대학원 융합공학과)

Abstract ▼ AI-Helper

The objective of this study was to determine the efficacy of a natural product of cherry tree (Prunus serrulata var. spontanea: PS) as a test substance for improving cytokine and ovalbumin-specific IgE using an ovalbumin-induced asthma disease model of 5-week-old male BALB/c mice. Lung tissue pathology was analyzed to confirm anti-inflammatory and asthmatic effects. As a result of examining the effect on changes in inflammatory cells in bronchoalveolar lavage fluid in an ovalbumin-induced asthma disease model by administering the PS sample, total cells, eosinophil, neutrophil, lymphocyte, and monocytes were significantly decreased. Concentrations of cytokine-based TNF-alpha and IL-4 and immunoglobulin E in serum were significantly increased in the asthma-inducing negative control group than in the normal group. However, high concentrations of PS decreased them. In histopathological examination of the lung tissue, it was confirmed that inflammatory cells infiltrated around the alveoli and bronchioles were increased in ovalbumin-induced asthma disease model. After administration of cherry tree extract, bronchiolar morphological changes such as mucosal thickening were slightly improved. From the above results, it was confirmed that extract of cherry tree significantly reduced inflammation expression and tissue damage in alveolar tissues. It was also confirmed that the cherry tree extract had an excellent efficacy in improving asthma inflammation.

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표/그림 (10)

그림 Fig. 1. Analysis of major metabolites in Prunus serrulata extract by liquid chromatogram. 1, methyl luteolin/ hispidulin; 2, galangin/ hydroxydaidzein/ trihydroxyisoflavone/ sulfurein/ aloe emodin/ lucidin /baicalein; 3, naringenin- 7-methyl ether; 4, methylapigenin; 5, methylapigenin/ methylgenistein; 6, sakuranetin; 7, shekanin; 8, genistein/ galangin/ apigenin/ hydroxydaidzein; 9, acacetin (methyl apigenin).
표 Table 1. Identification of major metabolites contributing the separation among sample groups in LC/MS
그림 Fig. 2. Changes of body weight in OVA-induced asthma mice. G1, normal group; G2, OVA-induced asthma group, G3, Asthma/PS 100 mg/kg; G4, Asthma/PS 200 mg/kg; G5, Asthma/PS 300 mg/kg; PS, Prunus serrulata var. spontanea.
그림 Fig. 3. Changes of food intake in OVA-induced asthma mice. G1, normal group; G2, OVA-induced asthma group, G3, Asthma/PS 100 mg/kg; G4, Asthma/PS 200 mg/kg; G5, Asthma/PS 300 mg/kg; PS, Prunus serrulata var. spontanea.
그림 Fig. 4. Effects of PS on inflammatory cells in bronchoalveolar lavage fluids of OVA-induced asthma mice. G1, normal group; G2, OVA-induced asthma group, G3, Asthma/PS 100 mg/kg; G4, Asthma/PS 200 mg/kg; G5, Asthma/PS 300 mg/kg; PS, Prunus serrulata var. spontanea; WBC, white blood cell; NEU, neutrophil; LYM, lymphocyte; MONO, monocytes; EOS, eosinophil; BASO, basophil. *p<0.05 compared to G1 by student's t-test. ^a,bMeans with the different letters among asthma groups are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 5. Effects of PS on the serum levels of TNF-alpha and IL-4 in OVA-induced asthma mice. G1, normal group; G2, OVA-induced asthma group, G3, Asthma/PS 100 mg/kg; G4, Asthma/PS 200 mg/kg; G5, Asthma/PS 300 mg/kg; PS, Prunus serrulata var. spontanea; *p<0.05 compared to G1 by student's t-test. ^a,bMeans with the different letters among asthma groups are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 6. Effects of PS on the serum levels of IgE in OVA-induced asthma mice. G1, normal group; G2, OVA-induced asthma group, G3, Asthma/PS 100 mg/kg; G4, Asthma/PS 200 mg/kg; G5, Asthma/PS 300 mg/kg; PS, Prunus serrulata var. spontanea; *p<0.05 compared to G1 by student's t-test. a,bMeans with the different letters among asthma groups are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 7. Effects of PS on the serum biochemistry in OVA-induced asthma mice. G1, normal group; G2, OVA-induced asthma group, G3, Asthma/PS 100 mg/kg; G4, Asthma/PS 200 mg/kg; G5, Asthma/PS 300 mg/kg; PS, Prunus serrulata var. spontanea; *p<0.05 compared to G1 by student's t-test. ^a,bMeans with the different letters among asthma groups are significantly different (p<0.05) by Duncan's multiple range tests.
그림 Fig. 9. Effects of PS on histopathological changes in lung tissues of OVA-induced asthma mice. G1, normal group; G2, OVA-induced asthma group, G3, Asthma/PS 100 mg/kg; G4, Asthma/PS 200 mg/kg; G5, Asthma/PS 300 mg/kg; PS, Prunus serrulata var. spontanea; Lung tissues were stained with H&E and observed by microscope (×200 original magnification). Arrows represent proliferation on mucosal epithelium and hypertrophy on submucosal smooth muscle.
그림 Fig. 8. Changes in weight of lung in OVA-induced asthma mice. G1, normal group; G2, OVA-induced asthma group, G3, Asthma/PS 100 mg/kg; G4, Asthma/PS 200 mg/kg; G5, Asthma/PS 300 mg/kg; PS, Prunus serrulata var. spontanea; *p<0.05 compared to G1 by student's t-test. ^a,bMeans with the different letters among asthma groups are significantly different (p<0.05) by Duncan's multiple range tests.

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