[논문]2'-Fucosyllactose가 마우스 배변 및 장내 미생물에 미치는 영향

김한해; 김연지; 김광연; 신철수; 윤종원; 전선민; 김보미; 방정수; 김경호

doi:10.9799/ksfan.2023.36.3.193

[국내논문] 2'-Fucosyllactose가 마우스 배변 및 장내 미생물에 미치는 영향
Effects of 2'-Fucosyllactyose on Defecation and Intestinal Microbiota in Mice 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.36 no.3, 2023년, pp.193 - 201

김한해 (한국한의학연구원 UST) , 김연지 (한국한의학연구원) , 김광연 (한국한의학연구원) , 신철수 ((주)에이피테크놀로지) , 윤종원 ((주)에이피테크놀로지) , 전선민 ((주)에이피테크놀로지) , 김보미 ((주)에이피테크놀로지) , 방정수 ((주)에이피테크놀로지) , 김경호 (한국한의학연구원)

Abstract ▼ AI-Helper

Prebiotics are known as components of intestinal microbiota that can improve and maintain human health status by stimulating the growth and activity of the intestinal tract as a method of controlling the intestinal environment. In this study, we examined whether 2'-fucosyllactose (FL) could affect intestinal microbial population and bowel activity. Water content and frequency of mouse feces were increased in the 2'-FL treated group at a high concentration (1,000 mg/kg), with brightness of the color enhanced and physical properties diluted. In addition, intestinal microbial analysis showed that harmful bacteria Clostridium and Staphylococcus strains were decreased and beneficial bacteria such as Lactobacillus strains were markedly increased in the group treated with a high concentration of 2'-FL compared to those in the control group. These findings suggest that administration of 2'-FL can maintain healthy bowel activity by reducing harmful bacteria population and improving diluted physical properties.

주제어

표/그림 (7)

표 Table 1. Changes in body weight(g) of mice
표 Table 2. Effects of 2'-FL on the stool frequency per 1 hour
그림 Fig. 1. Effects of 2'-FL on the stool frequency. Vehicle mice are gavaged saline and had free access to drinking water. HMO, 2'-FL-Low or 2'-FL-How mice were treated with 600 and 1,000 mg/kg 2'-FL, respectively, stool was observed once every 4 weeks. Data are shown as the mean±SEM, n=8, *p<0.05, vs. control (2-way ANOVA test).
표 Table 3. Effects of 2'-FL on the stool water contents
그림 Fig. 2. Effects of 2'-FL on the water contents of stool. Vehicle mice are gavaged saline and had free access to drinking water. HMO, 2'-FL-Low or 2'-FL-High mice were treated with 600 mg/kg 2'-FL or 1,000 mg/kg 2'-FL, respectively, 11 consecutive weeks from 1 week. Data are shown as the mean±SEM, n=8, *p<0.05, vs. control (2-way ANOVA test)
그림 Fig. 3. Effects of 2'-FL on the physical characteristics of stool. The C57BL/6 mice (n=8) were treated with 2'-FLLow (600 mg/kg) and 2'-FL-High (1,000 mg/kg), and stool samples were collected twice and every 4 weeks.
그림 Fig. 4. Effects of 2'-FL on the changes of intestinal microbiom. The relative abundance (%) of the major bacteria in C57BL6/J mice small intestines distinguished by groups. (A) Bifidobacterium spp., (B) Lactobacillus spp., (C) Clostridium spp., (D) Staphylococcus spp. Data are shown as the mean±SEM, n=8, *p<0.05, vs. control (2-way ANOVA test). ¹⁾ CFU (colony forming unit)/g (feces weight).

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