[논문]갯완두(Lathyrus japonicus) 에탄올 추출물의 항산화 및 항염증 효능

박윤경; 이명석; 고석천; 이정민; 임미진; 김경우; 김현수; 김지율; 김철환; 원정혜; 이대성; 오건우

doi:10.15433/ksmb.2023.15.1.001

갯완두(Lathyrus japonicus) 에탄올 추출물의 항산화 및 항염증 효능
Antioxidant and anti-inflammatory activities of Lathyrus japonicus ethanol extracts 원문보기

한국해양바이오학회지 = Journal of marine bioscience and biotechnology, v.15 no.1, 2023년, pp.1 - 11

박윤경 (국립해양생물자원관) , 이명석 (국립해양생물자원관) , 고석천 (국립해양생물자원관) , 이정민 (국립해양생물자원관) , 임미진 (국립해양생물자원관) , 김경우 (국립해양생물자원관) , 김현수 (국립해양생물자원관) , 김지율 (국립해양생물자원관) , 김철환 (국립해양생물자원관) , 원정혜 (국립해양생물자원관) , 이대성 (국립해양생물자원관) , 오건우 (국립해양생물자원관)

Abstract ▼ AI-Helper

In the present study, the antioxidant and anti-inflammatory activities of ethanolic extracts from Lathyrus japonicus at concentrations of 50, 100, and 200 ㎍/mL were investigated in LPS-stimulated, RAW264.7 cells. Antioxidant properties were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays and ferric reducing antioxidant power assay. In addition, the production of reactive oxygen species (ROS) was measured using the 2',7'-dichlorofluorescein diacetate (DCFH-DA) probe by flow cytometry. To examine the anti-inflammatory activity of the extracts of L. japonicus, their effects on the levels of nitric oxide (NO); production of cytokines such as interleukin (IL)-1β, IL-10, and tumor necrosis factor-α (TNF-α); and the activities of enzymes such as inducible NOS (iNOS) and cyclooxygenase-2 (COX-2) were assessed. The IC50 values of the DPPH and ABTS radical scavenging assays were 476.09 ± 1.50 and 34.91 ± 0.37 ㎍/mL, respectively. In addition, L. japonicus extracts not only inhibited ROS production, but also the production of NO, IL-1β, and IL-10, and the activity of iNOS in a dose-dependent manner. In summary, the ethanolic extracts of L. japonicus could be used as a functional food additive and an anti-inflammatory agent owing to their antioxidant and anti-inflammatory activities

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표/그림 (5)

그림 Figure 1. Antioxidant activities such as (A) DPPH, ABTS radical scavenging and (B) FRAP of L. japonicus 70% EtOH extracts.
그림 Figure 2. Effect of L. japonicus 70% EtOH extracts on (A) cell viability, (B) NO production, and (C) PGE2 pruduction in LPS-stimulated RAW264.7 cells. Data are mean ± SD of three independent experiments. ^#p < 0.05 vs. the non-treated group, ^*p < 0.05 vs. the sample treated group.
그림 Figure 3. Effect of L. japonicus 70% EtOH extracts on ROS production in LPS-stimulated RAW264.7 cells. DCF fluorescence was investigated using flow cytometry. AA, ascorbic acid. Data are mean ± SD of three independent experiments. ^#p < 0.05 vs. the non-treated group, ^*p < 0.05 vs. the sample treated group.
그림 Figure 4. Effect of L. japonicus 70% EtOH extracts on (A) IL-1β, (B) IL-10, and (C) TNF-α production in LPS-stimulated RAW264.7 cells. Data are mean ± SD of three independent experiments. ^#p < 0.05 vs. the non-treated group, ^*p < 0.05 vs. the sample treated group.
그림 Figure 5. Effect of L. japonicus 70% EtOH extracts on (A) iNOS and (B) COX-2 concentration in LPS-stimulated RAW264.7 cells. Data are mean ± SD of three independent experiments. ^#p < 0.05 vs. the non-treated group, ^*p < 0.05 vs. the sample treated group.

참고문헌 (39)

Ohtsuki, T., Setoguchi, H. 2011. Geographical differentiation in leaf thickness between coastal and freshwater populations of the coastal plant Lathyrus japonicus (Fabaceae). Bull. Natl. Sci. Mus. Ser. B 37, 127-132.？
Ohtsuki, T., Murai, Y., Iwashina, T., Setoguchi, H. 2013. Geographical differentiation inferred from flavonoid content between coastal and freshwater populations of the coastal plant Lathyrus japonicus (Fabaceae). Biochem. Syst. Ecol. 51, 243-250.？

상세보기
Nakai, Z., Kondo, T., Akimoto, S. 2011. Parasitoid attack of the seed-feeding beetle Bruchus loti enhances the germination success of Lathyrus japonicus seeds. Arthropod Plant Interact. 5, 227-234.？

상세보기
Ohtsuki, T., Kaneko, Y., Setoguchi, H. 2011. Isolated history of the coastal plant Lathyrus japonicus (Leguminosae) in Lake Biwa, an ancient freshwater lake. AoB plants 2011.？

상세보기
Randall, R. 1977. The past and present status and distribution of Sea Pea, Lathyrus japonicus, Willd., in the British Isles. Watsonia 11, 247-251.？
Kim, H. M., Han, S., Choi, K., Ku, J. J., Park, K. W., Cho, E. J., Lee, S. H. 2012. Aldose reductase inhibitory activity of the methanol extracts from Korean folk plants. Korean J. Agric. Sci. 39(2), 169-175.？
Lee, H. J., Kim, Y. A., Ahn, J. W., Na, H. J., Kim, H. M., Seo, Y. 2006. Screening of Korean marine plants for their inhibitory effect on histamine release from RPMC in vitro. Biotechnol. Bioprocess Eng. 11, 80-83.？
Lee, H. J., Kim, Y. A., Lee, J. I., Lee, B. J., Seo, Y. W. 2007. Screening of Korean marine plants extracts for inhibitory activity on protein tyrosine phosphatase 1B. J. Appl. Biol. Chem. 50(2), 74-77.？

원문보기 상세보기
Yu, H. N., Cho, K. H., Sok, D. E., Jeong, T. S. 2003. Inhibitory Effects of Natural Plant Extracts on Lipoprotein-Associated Phospholipase $ A_2 $, $,？ $, Platelet-Activating Factor Acetylhydrolase. Korean J. Pharmacogn. 34(1), 100-108.？
Lee, I. S., Dat, N. T., Cai, X. F., Shen, G. H., Kim, Y. H. 2003. Inhibitory effects of natural products against NFAT (nuclear factor of activated T cells) transcription factor. Korean J. Pharmacogn. 34(2), 150-155.
11 Miao, L., Clair, D. K S. 2009. Regulation of superoxide dismutase genes: implications in disease. Free Radic. Biol. Med. 47(4), 344-356.？

상세보기
Valentao, P., Fernandes, E., Carvalho, F., Andrade, P. B., Seabra, R. M., de Lourdes Bastos, M. 2002. Antioxidant activity of Hypericum androsaemum infusion: scavenging activity against superoxide radical, hydroxyl radical and hypochlorous acid. Biol. Pharm. Bull. 25(10), 1320-1323.？

상세보기
Hamasaki, T., Kashiwagi, T., Imada, T., Nakamichi, N., Aramaki, S., Toh, K., Morisawa, S., Shimakoshi, H., Hisaeda, Y., Shirahata, S. 2008. Kinetic analysis of superoxide anion radical-scavenging and hydroxyl radical-scavenging activities of platinum nanoparticles. Langmuir 24(14), 7354-7364.？

상세보기
Vrchovska, V., Sousa, C., Valentao, P., Ferreres, F., Pereira, J. A., Seabra, R. M., Andrade, P. B. 2006. Antioxidative properties of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves against DPPH, superoxide radical, hydroxyl radical and hypochlorous acid. Food Chem. 98(3), 416-425.？

상세보기
Na, E. J., Jang, H. H., Kim, G. R., Na, E. J., Jang, H. H., Kim, G. R. 2016. Review of recent studies and research analysis for anti-oxidant and anti-aging materials. Asian J. Beauty Cosmetol. 14(4), 481-491.？
de Cassia da Silveira e Sa, R., Andrade, L. N., de Sousa, D. P. 2013. A review on anti-inflammatory activity of monoterpenes. Molecules 18(1), 1227-1254.？

상세보기
Park, C. M., Park, J. Y., Noh, K. H., Shin, J. H., Song, Y. S. 2011. Taraxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-κB modulation in RAW 264.7 cells, J. Ethnopharmacol. 133(2), 834-842.？
Mueller, M., Hobiger, S., Jungbauer, A. 2010. Anti-inflammatory activity of extracts from fruits, herbs and spices. Food Chem. 122(4), 987-996.？

상세보기
Ahn, S., Singh, P., Jang, M., Kim, Y. J., Castro-Aceituno, V., Simu, S. Y., Kim, Y. J., Yang, D. C. 2018. Gold nanoflowers synthesized using Acanthopanacis cortex extract inhibit inflammatory mediators in LPS-induced RAW264. 7 macrophages via NF-κB and AP-1 pathways. Colloids Surf. B: Biointerfaces 162, 398-404.？
EYIIS, E., PEKGOZ, A. K. 2021. Radical Scavenging Activity of Some Lathyrus Taxa Distributed in Burdur-Isparta Regio. Suleyman Demirel univ. fen bilim. enst. derg. 25(1), 121-126.？
Pastor-Cavada, E., Juan, R., Pastor J. E.,, Alaiz, M., Vioque, J. 2009. Antioxidant activity of seed polyphenols in fifteen wild Lathyrus species from South Spain. LWT-Food Sci. Technol. 42(3), 705-709.？
Kim, J. Y., Lee, Y. J., Kim, W. S., Moon, S. K., Kim, Y. T. 2022. Antioxidant and Physiological Activities of Different Solvent Extracts of Cnidium japonicum. Korean J. Fish. Aquat. Sci. 55(3), 310-318.？
Kim, S. S., Cha, H. C. 2017. Comparison of the total phenolic and flavonoid contents and antioxidant activities of four kinds of sand dune plants living in Taean, Korea. Korean J. Plant Res. 30(1), 8-16.？
Kim, D. G., Shin, J. H., Kang M. J. 2018. Antioxidant and anti-inflammatory activities of water extracts and ethanol extracts from Portulaca oleracea L. Korean J. Food Preserv. 25(1), 98-106.？
Yoon, M. H., Lim, C. H., Oh, J. H., Lee, J. C., Choi, Y. W. 1997. Screening of Inhibitors of platelet aggregation from edible plants. Korean J. Agric. Sci. 24(2), 267-274.？
Choi, J. M., Choi, J. Y., Kim, H. M., Choi, K., Ku, J., Park, K. W., Lee, S., Cho, E. J. 2011. Antioxidative Activity and Protective Effect from Gastric Cancer of Korean Folk Plants. Cancer Prev. Res. 16(4), 387-394.？
Lee, H., Kim, Y., Ahn, J., Lee, B., Moon, S. 2004. Screening of peroxynitrite and DPPH radical scavenging activities from salt marsh plants, Korean J. Biotechnol. Bioeng. 19(1), 57-61.？
Lee, S. E., Sung, J. S., Jang, I. B., Kim, G. S., Ahn, T. J., Han, H. S., Kim, J. E., Kim, Y. O., Park, C. B., Cha, S. W. 2008. Investigation on antioxidant activity in plant resources. Korean J. Crop Sci. 16(5), 356-370.？
Zhao, Y., Vanhoutte, P. M., Leung, S. W. 2015. Vascular nitric oxide: Beyond eNOS. J. Pharmacol. Sci. 129(2), 83-94.？
Lee, H. J., Jeong, D. E., Gang, J. E., Sim, M. O., Seong, T. K., Woo, K. W., An, B., Jung, H. K., Cho, H. W. 2018. Quantitative Analysis of Chemical Composition and In Vitro Anti-oxidant and Anti-inflammatory Properties of Asparagus oligoclonos. Korean J. Pharmacogn. 49(2), 138-144.？
Rastogi, R. P., Singh, S. P., Hader, D. P., Sinha, R. P. 2010. Detection of reactive oxygen species (ROS) by the oxidant-sensing probe 2', 7'-dichlorodihydrofluorescein diacetate in the cyanobacterium Anabaena variabilis PCC 7937. Biochem. Biophys. Res. Commun. 397(3), 603-607.？

상세보기
Lee, E. S., Ju, H. K., Moon, T. C., Lee, E., Jahng, Y., Lee, S. H., Son, J. K., Baek, S. H., Chang, H. W. 2004. Inhibition of nitric oxide and tumor necrosis factor-α (TNF-α) production by propenone compound through blockade of nuclear factor (NF)-κB activation in cultured murine macrophages. Biol. Pharm. Bull. 27(5), 617-620.？
Kiemer, A. K., Hartung, T., Huber, C., Vollmar, A. M. 2003. Phyllanthus amarus has anti-inflammatory potential by inhibition of iNOS, COX-2, and cytokines via the NF-κB pathway. J. Hepatol. 38(3), 289-297.？
Chu, H., Tang, Q., Huang, H., Hao, W., Wei, X. 2016. Grape-seed proanthocyanidins inhibit the lipopolysaccharide-induced inflammatory mediator expression in RAW264. 7 macrophages by suppressing MAPK and NF-κb signal pathways. Environ. Toxicol. Pharmacol. 41, 159-166.？
Zhu, J., Luo, C., Wang, P., He, Q., Zhou, J., Peng, H. 2013. Saikosaponin A mediates the inflammatory response by inhibiting the MAPK and NF-κB pathways in LPS-stimulated RAW 264.7 cells. Exp. Ther. Med., 5(5), 1345-1350.？
Yen, J. H., Yang, D. J., Chen, M. C., Hsieh, Y. F., Sun, Y. S., Tsay, G. J. 2010. Glycine tomentella Hayata inhibits IL-1β and IL-6 production, inhibits MMP-9 activity, and enhances RAW264. 7 macrophage clearance of apoptotic cells. J. Biomed. Sci. 17(1), 1-9.？
Chen, G., Li, K. K., Fung, C. H., Liu, C. L., Wong, H. L., Leung, P. C., Ko, C. H. 2014. Er-Miao-San, a traditional herbal formula containing Rhizoma Atractylodis and Cortex Phellodendri inhibits inflammatory mediators in LPS-stimulated RAW264. 7 macrophages through inhibition of NF-κB pathway and MAPKs activation. J. Ethnopharmacol. 154(3), 711-718.
38 Sierra, A., Navascues, J., Cuadros, M. A., Calvente, R., Martin-Oliva, D., Ferrer-Martin, R. M., Martin-Estebane, M., Carrasco, M. C., Marin-Teva, J, L. 2014. Expression of inducible nitric oxide synthase (iNOS) in microgliaof the developing quail retina. PLoS One 9(8), e106048.？

상세보기
Eibl, G., Bruemmer, D., Okada, Y., Duffy, J. P., Law, R. E., Reber, H. A., Hines, O. J. 2003. PGE 2 is generated by specific COX-2 activity and increases VEGF production in COX-2-expressing human pancreatic cancer cells. Biochem. Biophys. Res. Commun. 306(4), 887-897.

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