[논문]강원도 가시오갈피의 식물 부위 또는 추출 용매 조건에 따른 항염증 효과

박준규; 부민아; 안수진; 신수진; 박진봉; 최호영; 이경진

doi:10.6116/kjh.2023.38.4.21

강원도 가시오갈피의 식물 부위 또는 추출 용매 조건에 따른 항염증 효과
Anti-inflammatory Effect of Eleutherococcus senticosus Extracts from Gangwon-do by Plant parts or Solvents 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.38 no.4, 2023년, pp.21 - 29

박준규 (경희대학교 대학원 한의과대학 기초한의과학과) , 부민아 (경희대학교 대학원 한의과대학 기초한의과학과) , 안수진 (경희대학교 한의과대학 한의학과) , 신수진 (경희대학교 한의과대학 한의학과) , 박진봉 (경희대학교 한의과대학 약리학교실) , 최호영 (경희대학교 한의과대학 본초학교실) , 이경진 (경희대학교 한의과대학 본초학교실)

Abstract ▼ AI-Helper

Background : The purpose of this study was to investigate the anti-inflammatory properties of stems and leaves of Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (ES) from Gangwon-do. Methods and Results : Stems and leaves of ES were collected from two areas in Gangwon-do: Cheorwon-gun and Samcheok-si. Samples were extracted with water by using the pressurized liquid extraction method and with 70% prethanol A by using the heat reflux extraction method. The anti-inflammatory effects of ES were evaluated through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT), lactate dehydrogenase(LDH) assay, nitric oxide(NO) assay, enzyme-linked immunosorbent assay(ELISA), and Western blot analysis in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). 1) Results showed that ES leaf extractions were not cytotoxic at a concentration of up to 30 ㎍/㎖. The leaves of 70% prethanol A extractions of ES(30 ㎍/㎖) inhibited NO, interleukin-6(IL-6), and tumor necrosis factor-α(TNF-α) production and decreased the protein level of cyclooxygenase 2(COX-2). There was no significant change in the protein level of inducible nitric oxide synthase(iNOS). The stem extractions of ES did not exhibit anti-inflammatory effects. Conclusions : In this study, the leaves of 70% prethanol A extractions of ES demonstrated anti-inflammatory effect on RAW 264.7 macrophages. The 70% prethanol A extractions have a relatively higher anti-inflammatory effect on RAW 264.7 macrophages than water extractions.

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표/그림 (5)

표 Table 1. Extraction yields of various plant E. senticosus (ES) by water and 70% prethanol A solvents.
그림 Fig. 1. Cell viability and LDH release of ES on LPS-induced RAW 264.7 cells. Murine macrophage cell line(2×10⁴) was incubated for 24 h with three varying concentrations(10, 30, and 100 ㎍/㎖) of ES. Effect of A; water and B; 70% prethanol A extracts on cell viability by MTT assay(n=5). Effect of C; water and D; 70% prethanol A extracts on cell viability by LDH assay(n=3). All data represent the mean ± S.D. of experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus LPS treated group(LPS+) and ^###p < 0.001 versus H₂O₂ group. CSW, ES stem water extract from Cheorwon-gun; CLW, ES leaf water extract from Cheorwon-gun; SSW, ES stem water extract from Samcheok-si; SLW, ES leaf water extract from Samcheok-si; CS70, ES stem 70% prethanol A extract from Cheorwon-gun; CL70, ES leaf 70% prethanol A extract from Cheorwon-gun; SS70, ES stem 70% prethanol A extract from Samcheok-si; SL70, and ES leaf 70% prethanol A extract from Samcheok-si.
그림 Fig. 2. Effect of ES on NO production in LPS-induced RAW 264.7 cells. NO production was measured using the NO kit(n=3). All data represent the mean ± S.D. of experiments. *p < 0.05, **p < 0.01 versus LPS treated group(LPS+) and ^###p < 0.001 versus nontreated group (LPS-). 30 CSW, Stem water extract at a concentration of 30 ㎍/㎖ from Cheorwon-gun; 30 SSW, Stem water extract at a concentration of 30 ㎍/㎖ from Samcheok-si; 30 CLW, Leaf water extract at a concentration of 30 ㎍/㎖ from Cheorwon-gun; 30 SLW, Leaf water extract at a concentration of 30 ㎍/㎖ from Samcheok-si; 30 CL70, Leaf 70% prethanol A extract at a concentration of 30 ㎍/㎖ from Cheorwon-gun; 30 SL70, Leaf 70% prethanol A extract at a concentration of 30 ㎍/㎖ from Samcheok-si.
그림 Fig. 4. Effects of ES on iNOS and COX-2 protein expression in LPS-induced RAW 264.7 cells. iNOS and COX-2 protein expression was measured using Western blot analysis (n=3). All data represent the mean ± S.D. of experiments. *p < 0.05 versus LPS treated group(LPS+) and #p < 0.05, ^##p < 0.01 versus non-treated group (LPS-). 30 CL70, Leaf 70% prethanol A extract at a concentration of 30 ㎍/㎖ from Cheorwon-gun; 30 SL70, Leaf 70% prethanol A extract at a concentration of 30 ㎍/㎖ from Samcheok-si.
그림 Fig. 3. Effects of ES on TNF-α and IL-6 production in LPS-induced RAW 264.7 cells. TNF-α and IL-6 production were measured using the ELISA kits (n=3). All data represent the mean ± S.D. of experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus LPS treated group(LPS+) and ^###p < 0.001 versus non-treated group(LPS-). 30 CSW, Stem water extract at a concentration of 30 ㎍/㎖ from Cheorwon-gun; 30 SSW, Stem water extract at a concentration of 30 ㎍/㎖ from Samcheok-si; 30 CLW, Leaf water extract at a concentration of 30 ㎍/㎖ from Cheorwon-gun; 30 SLW, Leaf water extract at a concentration of 30 ㎍/㎖ from Samcheok-si; 30 CL70, Leaf 70% prethanol A extract at a concentration of 30 ㎍/㎖ from Cheorwon-gun; 30 SL70, Leaf 70% prethanol A extract at a concentration of 30 ㎍/㎖ from Samcheok-si.

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