[논문]황복의 마취에 미치는 수온 및 연령과 MS-222 농도의 영향

이승연; 송호경; 허성표; 김진형

doi:10.35399/isk.35.2.1

황복의 마취에 미치는 수온 및 연령과 MS-222 농도의 영향
Effect of Water Temperature, Fish Age, and MS-222 Concentration on the Anesthetization of River Pufferfish, Takifugu obscurus 원문보기

Korean journal of Ichthyology = 한국어류학회지, v.35 no.2, 2023년, pp.67 - 74

이승연 (극지연구소 생명과학연구본부) , (극지연구소 생명과학연구본부) , 송호경 (극지연구소 생명과학연구본부) , 허성표 (제주대학교 해양생명과학과) , 김진형 (극지연구소 생명과학연구본부)

초록
AI-Helper

황복(Takifugu obscurus)은 양식 어종 중 하나이며, 유전체 연구에서도 의미 있는 종이다. 양식 어종의 경우 어류 마취제를 사용하면 양식 과정에 있어서 어류를 좀 더 쉽게 다룰 수 있다. 그러나, 지금까지 황복의 효과적인 마취와 회복을 위해 필요한 적절한 조건에 대한 연구는 없었다. 본 연구는 가장 일반적인 어류 마취제인 MS222를 이용하여 황복을 마취하는 데 필요한 연령, 수온 및 마취 농도 등 최적의 조건을 파악하는 데 그 목적이 있다. 실험에서는 세 가지 다른 수온(20℃, 24℃, 28℃) 및 마취 농도(125 mg/L, 150 mg/L, 175 mg/L)와 두 가지 다른 어류 연령(1년생과 2년생)을 테스트하였다. 실험 결과 황복의 적절한 마취 조건은 24℃와 28℃에서 MS-222 150 mg/L에서 175 mg/L까지의 범위였다. 2년생 어류의 경우도 28℃에서 MS-222 150 mg/L에서 175 mg/L의 범위를 보였다. 부작용과 위험을 최소화하기 위한 효과적인 마취 농도는 1년생 어류의 경우 24℃에서 MS-222 150 mg/L, 2년생 어류의 경우 28℃에서 MS-222 175 mg/L가 권장된다.

Abstract ▼ AI-Helper

The river pufferfish (Takifugu obscurus) is a valuable species in aquaculture and genetic studies. Usage of fish anesthetics aids in the easier handling of fish during aquaculture. However, there are no studies on appropriate conditions required for effective anesthetization of pufferfish. This study aims to determine the optimal conditions (fish age, water temperature, anesthetic concentration) needed for the most common fish anesthesia, MS-222, to anesthetize T. obscurus. We tested three different water temperatures (20℃, 24℃, and 28℃), three different anesthetic concentrations (125 mg/L, 150 mg/L, and 175 mg/L), and two different fish ages (one- and two-year-old). Appropriate anesthetization conditions for T. obscurus ranged from 150 mg/L to 175 mg/L of MS-222 at 24℃ to 28℃ for one-year-old fish. For two-year-old fish, the appropriate conditions ranged from 150 mg/L to 175 mg/L of MS-222 at 28℃. However, to minimize side effects and risks, 150 mg/L of MS-222 at 24℃ for one-year-old fish and 175 mg/L of MS-222 at 28℃ for two-year-old fish are recommended for effective anesthetization.

주제어

표/그림 (4)

표 Table 1. Experimental conditions
표 Table 2. The anesthesia and recovery time at different temperatures and MS-222 concentrations
그림 Fig. 1. The anesthesia time and recovery time of one- and two-yearold fish at different water temperatures. Different letters indicate the significant difference of these two groups (P<0.05).
그림 Fig. 2. The anesthesia time graph (a) and recovery time graph (b). The larger bubble, the longer it took. The overall trend in the 3D graph. First, in (a) graph, it can be seen that the more age, the lower the anesthetic concentration, the lower the temperature, and the longer the anesthesia time takes.

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