[논문]배추 품종들의 검은썩음병에 대한 저항성 특성

이수민; 이진주; 최용호; 김헌; 최경자

doi:10.5423/rpd.2023.29.2.158

배추 품종들의 검은썩음병에 대한 저항성 특성
Resistance Characteristics of Chinese Cabbage Cultivars to Black Rot 원문보기

Research in plant disease = 식물병연구, v.29 no.2, 2023년, pp.158 - 167

이수민 (한국화학연구원 친환경신물질연구센터) , 이진주 (한국화학연구원 친환경신물질연구센터) , 최용호 (한국화학연구원 친환경신물질연구센터) , 김헌 (한국화학연구원 친환경신물질연구센터) , 최경자 (한국화학연구원 친환경신물질연구센터)

초록
AI-Helper

Xanthomonas campestris pv. campestris (Xcc)에 의한 검은썩음병은 배추 재배에서 가장 심각한 피해를 입히는 병해 중 하나이다. 본 연구는 Xcc에 대한 배추의 저항성 특성을 조사하기 위하여 실험하였다. 먼저, Xcc 8개 균주의 병원력을 비교하기 위하여, 감수성인 배추 4개 품종에서 이들 균주에 의한 배추 검은썩음병 발생을 조사하였다. 실험에 사용한 Xcc 균주들은 균주들 간에 서로 다른 병원력을 보였다. 그리고 Xcc에 대한 저항성 및 중도저항성 22개 배추 품종을 선발하고, Xcc 8개 균주를 사용하여 이들 배추 품종의 검은썩음병 발생을 조사한 결과, 배추 품종들의 평균 검은썩음병 병반면적률은 Xcc 균주들의 병원력에 따라 증가하였다. 게다가 실험한 각 배추 품종들의 검은썩음병 발생은 실험한 균주들의 병원력에 비례하여 증가하였으며, Xcc 균주들에 대해 저항성인 배추 품종의 수는 균주들의 병원력에 반비례하게 감소하였다. 이상의 결과로부터 Xcc에 대한 배추의 저항성은, 병원균의 레이스 분화가 아니라 병원균의 병원력에 영향받는다고 생각되었다.

Abstract ▼ AI-Helper

Black rot of Chinese cabbage caused by Xanthomonas campestris pv. campestris (Xcc) is one of the most severe diseases in crop cultivation. To define the resistance characteristics of Chinese cabbage to Xcc, we tested the virulence of eight Xcc isolates in four susceptible cultivars of Chinese cabbage. The isolates of Xcc showed different the virulence on the cultivars. On the other hand, we selected 22 resistant or moderately resistant cultivars of Chinese cabbage to Xcc and tested the occurrence of black rot on the cultivars caused by the isolates of Xcc. Mean disease severity of black rot on the Chinese cabbage caused by each isolate was also positively correlated with the virulence of Xcc isolates. Furthermore, the development of black rot in each cultivar increased according to virulence of Xcc isolates. The number of resistant cultivars of Chinese cabbage to eight isolates of Xcc decreased according to the virulence increase of bacteria. Taken together, these results suggest that resistance of Chinese cabbage cultivars to Xcc is likely affected by the virulence of Xcc isolates, but not result from race differentiation.

주제어

표/그림 (5)

그림 Fig. 1. Development of black rot on four susceptible cultivars of Chinese cabbage caused by eight isolates of Xanthomonas campestris pv. campestris. (A) 'CR Nongsim'. (B) 'Chunkwang'. (C) 'CR Haruyosi'. (D) 'Chunyeon'. Ten-day-old seedlings were inoculated with a bacterial suspension of each isolate at a concentration of 1.0×108 cfu/ml by spray method. The inoculated plants were incubated in a humidity chamber at 30ºC for 48 hr and then transferred to a growth chamber at 25ºC and relative humidity 80% with 12 hr light a day. After three, four, six, and eight days, disease severity was measured as percentage of diseased leaf area of each plant. Each value represents the mean disease severity of two runs with 10 replicates each. Values labeled with the same letter within each day after inoculation are not significantly different based on Duncan's multiple range test at P=0.05.
그림 Fig. 2. Area under the disease progress curve (AUDPC) of black rot on four Chinese cabbage cultivars caused by eight isolates of Xanthomonas campestris pv. campestris and AUDPC were calculated using the following equations. AUDPC=Σ n i=1 [t(i+1)–ti]×[DS(i+1)+DSi]/2; n=number of assessments, ti=number of days elapsed from the inoculation day to the assessment date i, DSi=disease severity (%) on assessment date i. Each value represents the mean AUDPC of two runs with 10 replicates each. Values labeled with the same letter within each cultivar are not significantly different based on Duncan’s multiple range test at P=0.05.
표 Table 1. Occurrence of black rot on 23 Chinese cabbage cultivars caused by 8 isolates of Xanthomonas campestris pv. campestris^a
표 Table 2. Resistance response of 20 Chinese cabbage cultivars to 8 isolates of Xanthomonas campestris pv. campestris^a
표 Table 3. The number of resistant, moderately resistant and susceptible cultivars of Chinese cabbage to each isolate of Xanthomonas camp-estris pv. campestris

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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