[논문]향사평위산의 항염증 효과 및 이상지질혈증 개선 작용에 대한 연구

채한나; 천세은; 신용진

doi:10.22246/jikm.2023.44.3.523

향사평위산의 항염증 효과 및 이상지질혈증 개선 작용에 대한 연구
A Study on the Anti-Inflammatory Effect and Improvement of Dyslipidemia on Hyangsapyeongwi-san 원문보기

대한한방내과학회지 = The journal of internal Korean medicine, v.44 no.3, 2023년, pp.523 - 535

채한나 (원광대학교 한의과대학 내과학교실) , 천세은 (원광대학교 전주한방병원 내과학교실) , 신용진 (원광대학교 한의과대학 내과학교실)

Abstract ▼ AI-Helper

Objectives: This study aims to investigate the anti-inflammatory effect and improvement of dyslipidemia on Hyangsapyeongwi-san. Methods: In this study, HUVEC cells were cultured and treated with Hyangsapyeongwi-san to measure intracellular KLF2, eNOS, MCP-1, ICAM-1, and VCAM-1 gene expression levels related to anti-inflammation. The weight of experimental animals administered with Hyangsapyeongwi-san was measured, blood samples were biochemically analyzed, and liver tissues were reviewed to research histological changes. Results: Gene expression levels in the cells treated with Hyangsapyeongwi-san generally showed a meaningful anti-inflammatory effect. The body weight of the experimental animals decreased, and total cholesterol, triglyceride, and LDL-cholesterol in the blood generally declined while HDL-cholesterol tended to increase. Fat accumulation between hepatocytes was also reduced after the administration of Hyangsapyeongwi-san. Conclusions: This study confirmed that Hyangsapyeongwi-san has the effect of suppressing vascular inflammatory responses through the regulation of genes involved in the vascular inflammatory process and improving dyslipidemia through the reduction of blood lipids and weight loss.

주제어

표/그림 (8)

표 Table 1. The Prescription of HPS
표 Table 2. The Ingredients of Normal Diet and High Cholesterol Diet
표 Table 3. Real-Time PCR Primer Sequences
그림 Fig. 1. Cell viability of HPS in HUVEC. HUVEC were treated by 50, 100, 200, 400, and 600 μg/ml of HPS for 24 h. Treated cells were reacted by EZ-Cytox for 30 min and then absorbance were measured at 450 nm using micro plate reader. Cell viability were calculated as percentage relative to the control. The result were presented by the mean±standard error of mean from three independent experiments. Control : 100.00±3.06, 50 μg/ml of HPS : 100.03±1.65, 100 μg/ml of HPS : 100.08±2.46, 200 μg/ml of HPS : 100.29±2.93, 400 μg/ml of HPS : 100.74±3.54, 600 μg/ml of HPS : 79.31±2.69 (Unit : %).
그림 Fig. 2. Effects of HPS about KLF2, eNOS, MCP-1, ICAM-1 and VCAM-1 mRNA expression level in HUVEC. HUVEC were treated 50, 100, 200, and 400 μg/ml of HPS, with 10 ng/ml TNF-α for 12 h. The mRNA expression level were measured using a real-time PCR (qPCR). The result were presented by the mean±standard error of mean from three independent experiments. (Significance of results, * : p<0.05, ** : p<0.01, *** : p<0.001 compared to control). (A) KLF2, (B) eNOS, (C) MCP-1, (D) ICAM-1, (E) VCAM-1.
그림 Fig. 3. Effects of HPS about body weight in dyslipidemia mice model. The result were presented by the mean±standard error of mean (Significance of results, ** : p<0.01, *** : p<0.001 compared to control). Normal : non-dyslipidemia mice model (n=6), Control : dyslipidemia mice model+distilled water (n=6), Low : dyslipidemia mice model+HPS 200 mg/kg (n=6), High : dyslipidemia mice model+HPS 400 mg/kg (n=6).
그림 Fig. 4. Effects of HPS about total cholesterol, LDL-cholesterol, HDL-cholesterol and triglyceride level in serum of dyslipidemia mice model. The result were presented by the mean±standard error of mean (Significance of results, ** : p<0.01, *** : p<0.001 compared to control). Normal : non-dyslipidemia mice model (n=6), Control : dyslipidemia mice model+distilled water (n=6), Low : dyslipidemia mice model+HPS 200 mg/kg (n=6), High : dyslipidemia mice model+HPS 400 mg/kg (n=6). (A) Total cholesterol, (B) LDL-cholesterol, (C) HDL-cholesterol, (D) Triglyceride
그림 Fig. 5. Effects of HPS about histological changes in liver tissue of dyslipidemia mice model. Liver tissue were stained with hematoxylin and eosin (H&E). Hepatocytes fat accumulation was pointed by black arrows. Normal : non-dyslipidemia mice model, Control : dyslipidemia mice model+distilled water, Low : dyslipidemia mice model+HPS 200 mg/kg, High : dyslipidemia mice model+HPS 400 mg/kg.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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