[논문]항생제 내성 Cutibacterium acnes에 대한 감태(Ecklonia cava) 추출물의 항균 시너지 효과

김은송; 류주원; 김효빈; 송호수; 윤나영; 심길보; 황혜진; 김영목; 엄성환

doi:10.5657/kfas.2023.0293

항생제 내성 Cutibacterium acnes에 대한 감태(Ecklonia cava) 추출물의 항균 시너지 효과
Addictive Effects of Ecklonia cava Methanol and its Solvent-soluble Extract Against Antibiotic-resistant Cutibacterium acnes Causing Acne Vulgaris 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.56 no.3, 2023년, pp.293 - 299

김은송 (동의대학교 식품공학과) , 류주원 (동의대학교 식품공학과) , 김효빈 (동의대학교 식품공학과) , 송호수 (영산대학교 조리예술학부) , 윤나영 (국립수산과학원 식품위생가공과) , 심길보 (부경대학교 식품공학과) , 황혜진 (동의대학교 식품영양학과) , 김영목 (부경대학교 식품공학과) , 엄성환 (동의대학교 식품공학과)

Abstract ▼ AI-Helper

Acne vulgaris is often associated with acne-related bacteria such as Cutibacterium acnes. In this study, we investigated the antibacterial effects of the methanol extract of the algae Ecklonia cava and its solvent-soluble extract against C. acnes. Among five solvent fractions, the ethyl acetate (EtOAc) fraction exhibited the strongest antibacterial activity against C. acnes. Furthermore, the EtOAc-soluble extract exhibited the highest total phenolic contents among the five solvent fractions tested. The EtOAc subfraction 07 (Fr. 07) extract showed the highest antibacterial effect against C. acnes and isolated C. acnes along with minimum inhibitory concentration (MIC) ranging from 32 to 64 ㎍/mL. Additionally, MICs of antibiotics against antibiotic-resistant C. acnes strains were substantially reduced when antibiotics were combined with Fr. 07, suggesting that Fr. 07 restore the antibacterial activity of the antibiotics. The fractional inhibitory concentration indices clearly revealed an additive synergistic effect of Fr. 07 with antibiotics. The results of the present study suggest a potential role for E. cava in the control of infections related to acne vulgaris.

주제어

표/그림 (6)

그림 Fig. 1. Isolation and purification procedure of antimicrobial substances from Ecklonia cava. MeOH, Methanol; Hex, n-hexane; DCM, Dichloromethane; EtOAc, Ethyl acetate; BuOH, n-butanol; H₂O, Water.
$Table 1. Total polyphenolic (TP) contents in Ecklonia cava methanolic extract and its solvent-soluble fractions$ 표 Table 1. Total polyphenolic (TP) contents in Ecklonia cava methanolic extract and its solvent-soluble fractions
$Table 2. Antimicrobial activity of solvent fractions from Ecklonia cava against Cutibacterium acnes and isolated C. acnes$ 표 Table 2. Antimicrobial activity of solvent fractions from Ecklonia cava against Cutibacterium acnes and isolated C. acnes
$Table 3. Antibacterial activities of ethyl acetate sub-fractions from Ecklonia cava methanol extracts$ 표 Table 3. Antibacterial activities of ethyl acetate sub-fractions from Ecklonia cava methanol extracts
표 Table 4. Minimum inhibitory concentrations (MICs) of chloramphenicol, ciprofloxacin, nalidixic acid, oxacillin sodium, penicillin G, and tetracycline against Cutibacterium acnes and isolated C. acnes
$Table 5. Minimum inhibitory concentrations (MICs) and fractional inhibitory concentration (FIC) indices of ethyl acetate sub-fraction 07 in combination with antibiotics against Cutibacterium acnes$ 표 Table 5. Minimum inhibitory concentrations (MICs) and fractional inhibitory concentration (FIC) indices of ethyl acetate sub-fraction 07 in combination with antibiotics against Cutibacterium acnes

AI 본문요약
AI-Helper

문제 정의

본 연구에서는 여드름 원인균인 C. acnes의 감태 분획물에 대한 항균활성을 확인하였고, 항생제에 대한 내성을 가지고 있는 분리 균주들에 대한 감태 분획물의 첨가에 의한 항생제의 항균력 회복에 대한 연구를 실시하였다.

제안 방법

07과 C. acnes에 대한 내성을 갖는 항생제와의 시너지 효과를 비교 분석하였다.
최근 항생제의 대한 내성 발생과 부작용으로 인해 효과적이고 안전한 대체 항생제 역할을 할 수 있는 천연물 유래 물질에 대한 선호도가 증가하고 있다. 이에 식용해조류인 감태 추출물을 대상으로 여드름균에 대한 항균 효과를 실시하였다. 감태 추출물의 용매 분획물 중에서 감태 EtOAc 용매분획 추출물 sub-fr.
총 폴리페놀 함량은 Folin-Denis법을 일부 수정하여 측정하였다(Lee et al., 2011). 희석된 추출물 시료 0.

대상 데이터

본 실험에 사용된 건조분말 상태의 감태(E. cava)는 장명식품(Seoul, Korea)에서 구매하였다. 건조분말 감태 100 g당 1 L의 MeOH (DAEJUNG Chemical & Metals Co.

데이터처리

시료간 유의성 검정은 one-way ANOVA를 이용하여 분석하였으며 P<0.05에서 Duncan's multiple range test를 실시하여 각 군의 유의차 검정을 실시하였다.

이론/모형

상층액을 취하여 Versamax microplate reader (Molecular Devices, Sunnyvale, CA, USA)로 760 nm에서 흡광도를 측정하였다. 표준물질로 phloroglucinol (Sigma-Aldrich)의 표준검량곡선을 작성하여 추출물의 총 폴리페놀 함량을 검량선의 선형방정식을 사용하여 계산되었으며, mg phloroglucinol equivalent (PGE)/g으로 표시하였다.

성능/효과

acnes 및 분리균주에 대한 MIC 값이 32–64 μg/mL로 가장 뛰어난 항균활성을 나타내었다
0의 값을 나타내어 C. acnes균에 대해 additive synergy 효과를 나타내었다. 따라서, 본 연구를 통해 여드름과 관련된 질병의 대체 치료제로써 감태 추출물의 가능성을 확인할 수 있었다.
acnes균에 대해 additive synergy 효과를 나타내었다. 따라서, 본 연구를 통해 여드름과 관련된 질병의 대체 치료제로써 감태 추출물의 가능성을 확인할 수 있었다.

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