[논문]벌나무 추출물의 황생포도상구균에 대한 항균 효과와 항산화 활성

황진우; 박찬휘; 안해연; 장예원; 강현; 이성규

doi:10.5010/jpb.2022.49.4.339

벌나무 추출물의 황생포도상구균에 대한 항균 효과와 항산화 활성
Antibacterial, Antioxidant Activities of Acer tegmentosum Maxim Ethanol Extract Against Staphylococcus aureus 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.49 no.4, 2022년, pp.339 - 346

황진우 (Department of Medical Laboratory Science, College of Health Science, Dankook University) , 박찬휘 (Department of Medical Laboratory Science, College of Health Science, Dankook University) , 안해연 (Department of Medical Laboratory Science, College of Health Science, Dankook University) , 장예원 (Department of Medical Laboratory Science, College of Health Science, Dankook University) , 강현 (Department of Medical Laboratory Science, College of Health Science, Dankook University) , 이성규 (Department of Medical Laboratory Science, College of Health Science, Dankook University)

초록
AI-Helper

본 연구는 벌나무 에탄올 추출물의 황색포도상구균에 대한 항균 및 항산화 활성을 검증하였다. 총 폴리페놀 및 플라보노이드 함량, DPPH와 ABTS 라디칼 소거효능과 FRAP법을 사용한 환원력을 측정하여 벌나무 에탄올 추출물의 항산화활성을 검증하였고, Paper disc법, MIC 및 MBC법을 통하여 황색포도상구균에 대한 벌나무 에탄올 추출물의 항균 활성을 검증하였다. 총 폴리페놀 및 플라보노이드 함량은 각각 266.66 ㎍ GAE/mg와 6.46 ㎍ QE/mg이었다. DPPH와 ABTS 라디칼 소거능은 농도 의존적 소거능을 보였고, 벌나무 에탄올 추출물의 RC₅₀ 값은 각각 21.49, 12.81 ㎍/mL이었다. 환원력을 평가하기 위해 수행된 FRAP 분석에서 벌나무 추출물은 0.73 ± 0.19 mM FeSO₄ E/mg의 효능을 보여주었다. Paper disc법을 이용한 황색포도상구균에 대한 벌나무 추출물의 항균 활성은 2 mg에서 3 mm의 저해환을 보여주었으며, 최소 저해농도 연구에서는 1 mg/mL 이상의 농도에서 80% 이상의 억제력을 보여주었고, 16 mg/mL 이상의 농도에서 최소저해 농도를 확인할 수 있었다. 또한, 최소살균농도 연구에서는 32 mg/mL에서 최소살균농도를 확인할 수 있었다. 벌나무 추출물을 사용한 항산화 및 황색포도상구균에 대한 항균 활성을 확인한 결과 벌나무 추출물의 아토피피부염에 유효한 물질로서의 가능성을 확인할 수 있었다.

Abstract ▼ AI-Helper

This study investigates the antibacterial and antioxidant activities of the ethanol extract of Acer tegmentosum Maxim (EATM) against Staphylococcus aureus. The total polyphenol and flavonoid content, 1,1-diphenyl-2-picryl hydrazyl (DPPH) and 2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging effect, and reducing power of the ferric reducing antioxidant power (FRAP) method were measured to verify the antioxidant activity of the EATM. The antibacterial activity against S. aureus using the EATM was verified by the paper disc method, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. The total polyphenol and flavonoid contents were 266.66 ㎍ GAE/mg and 6.46 ㎍ QE/mg, respectively. The DPPH and ABTS radical scavenging activity showed a concentration-dependent scavenging activity. The RC50 values of the EATM were 21.49 and 12.81 ㎍/mL, respectively. A FRAP analysis was conducted for evaluating the reducing power of the EATM and an efficacy of 0.73 ± 0.19 mM FeSO4 E/mg was observed. The antibacterial activity of EATM against S. aureus, determined using the paper disc method, showed an inhibitory ring of 3 mm at 2 mg. The MIC was confirmed at a concentration of ≥ 16 mg/mL, while the MBC was confirmed at 32 mg/mL. As the EATM shows antioxidant and antibacterial activities against S. aureus, it can be used as an effective antidote against atopic dermatitis.

주제어

표/그림 (6)

그림 Fig. 1 Antibacterial activity of the extract of Acer tegmentosum Maxim against Staphylococcus aureus. (A) 2 mg, (B) 1 mg, and (C) 0.5 mg of the extract of Acer tegmentosum Maxim; 100 ng of penicillin G (D). Data are expressed as the mean ± SD (n = 3) of three replicates
표 Table 1 Total polyphenols and flavonoids of ethanol extracts of Acer tegmentosum Maxim
표 Table 2 DPPH and ABTS radical scavenging activities of ethanol extracts of Acer tegmentosum Maxim
표 Table 3 Reducing power of ethanol extracts of Acer tegmentosum Maxim
그림 Fig. 2 Determination of MIC of Acer tegmentosum Maxim against Staphylococcus aureus. Data are expressed as the mean ± SD (n = 3) of three replicates
그림 Fig. 3 Determination of MBC of Acer tegmentosum Maxim against Staphylococcus aureus. (A) 2 mg/mL, (B) 4 mg/mL, (C) 8 mg/mL, (D) 16 mg/mL, and (E) 32 mg/mL of the extract of Acer tegmentosum Maxim. Data are expressed as the mean ± SD (n = 3) of three replicates

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