[논문]인가목(Rosa acicularis Lindl.) 잎 추출물의 대식세포에서 자가포식 유도활성

최정원; 정진부

doi:10.7732/kjpr.2023.36.4.257

[국내논문] 인가목(Rosa acicularis Lindl.) 잎 추출물의 대식세포에서 자가포식 유도활성
Induction of Autophagy by Rosa acicularis Leaves Extracts in RAW264.7 Cells 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.36 no.4, 2023년, pp.257 - 263

최정원 (국립안동대학교 생약자원학과) , 정진부 (국립안동대학교 생약자원학과)

초록
AI-Helper

본 연구에서 인가목 잎 추출물이 RAW264.7 세포에서 면역자 극인자의 생성을 증가시키고 포식작용의 활성화를 유도한다는 것을 확인하였다. 게다가 인가목 잎 추출물은 TLR4 의존적 JNK와 PI3K/AKT 신호전달 활성화를 통해 자가포식을 유도한다는 것을 확인하였다. 대식세포의 활성화와 자가포식은 선천면역반응과 후천면역반응을 향상시킬 수 있는 주요 전략 중 하나이기 때문에 인가목 잎 추출물은 인체의 면역반응을 증가시킬 수 있는 건강기능식품이나 보조제로 활용될 수 있을 것으로 생각된다. 그러나 본 연구에서는 작용기전을 구명하기 위해 관련된 단일억제제를 사용하였기 때문에 명확한 작용기전 구명을 위해 다양한 억제제 또는 siRNA를 활용한 추가적 연구가 필요하다고 판단된다.

Abstract ▼ AI-Helper

Autophagy contributes to enhancing the immune system (innate and adaptive immune system) against foreign pathogens. Autophagy of macrophages is used as a major indicator for developing vaccine adjuvants to increase the adaptive immune response. In this study, water extracts from Rosa acicularis leaves (RAL) increased the production of immunostimulatory mediators and phagocytic activity in RAW264.7 cells. RAL increased p62/SQSTM1 expression. Inhibition of TLR4, JNK, and PI3K/AKT blocked RAL-mediated increase of p62/SQSTM1. RAL activated JNK and PI3K/AKT signaling. RAL-mediated activations of JNK and PI3K/AKT signaling were reversed by TLR4 inhibition. Taken together, it is believed that RAL-mediated autophagy may be dependent on activating via TLR4-dependent activation of JNK and PI3K/AKT signaling in macrophages.

Keyword

표/그림 (5)

표 Table 1. Sequence of oligonucleotide primers used for RT-PCR
그림 Fig. 1. Effect of RAL on macrophage activation in RAW264.7 cells. RAW264.7 cells were treated with RAL for 24 h. NO level (A), mRNA level (B), phagocytic activity (C) and cell viability (D) were measured by Griess assay, RT-PCR, neutral red assay and MTT assay, respectively.
그림 Fig. 2. The effect of RAL on the expression of p62/SQSTM1 in RAW264.7 cells. (A) RAW264.7 cells were treated with RAL (200 ㎍/mL) for the indicated times. (B) RAW264.7 cells were treated with RAL (200 ㎍/mL) at the indicated concentrations for 6 h. The level of p62/SQSTM1 was analyzed by Western blot analysis.
그림 Fig. 3. The effect of TLR2/4 on the RAL-mediated expression of p62/SQSTM1 in RAW264.7 cells. RAW264.7 cells were treated with RAL (200 ㎍/mL) in the absence or presence of C29 (TLR2 inhibitor, 100 nM) or TAK-242 (TLR4 inhibitor, 5 ㎍/mL) for 6 h. The level of p62/SQSTM1 was analyzed by Western blot analysis.
그림 Fig. 4. The effect of MAPK, NF-κB, and PI3K/AKT signaling on the RAL-mediated expression of p62/SQSTM1 in RAW264.7 cells. (A) RAW264.7 cells were pretreated with PD98059 (ERK1/2 inhibitor, 40 μM), SB203580 (p38 inhibitor, 40 μM), SP600125 (JNK inhibitor, 40 μM), BAY 11-7082 (NF-κB inhibitor, 20 μM), or LY294002 (PI3K inhibitor, 40 μM) for 2 h and then co-treated with RAL (200 ㎍/mL) for 6 h. (B) RAW264.7 cells were treated with RAL (200 ㎍/mL) for the indicated times. (C) RAW264.7 cells were treated with TAK-242 (TLR4 inhibitor, 5 ㎍/mL) for 2 h and then co-treated with RAL (200 ㎍/mL) for 30 min. The protein level was analyzed by Western blot analysis.

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