[논문]고지방 식이로 유도된 비만 생쥐에서 박하 Hydrosol의 항비만 효과

최수민; 김소영; 김영준; 우창훈; 김미려; 안희덕

doi:10.18325/jkmr.2023.33.3.33

고지방 식이로 유도된 비만 생쥐에서 박하 Hydrosol의 항비만 효과
Anti-Obesity Effects of Menthae Herba Hydrosol on High-Fat Diet Induced Obese Mice 원문보기

Journal of Korean Medicine Rehabilitation : JKMR = 한방재활의학과학회지, v.33 no.3, 2023년, pp.33 - 46

최수민 (대구한의대학교 한의과대학 한방재활의학과교실) , 김소영 (대구한의대학교 한의과대학 본초약리학교실) , 김영준 (대구한의대학교 한의과대학 한방재활의학과교실) , 우창훈 (대구한의대학교 한의과대학 한방재활의학과교실) , 김미려 (대구한의대학교 한의과대학 본초약리학교실) , 안희덕 (대구한의대학교 한의과대학 한방재활의학과교실)

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Objectives We investigated anti-obesity effects of Menthae Herba hydrosol in obese mice. Methods Animals were divided into four groups, and treatments were performed for 7 weeks. After the treatment, serum lipid profiles, weight and pathological morphology in liver, kidney, adipose tissue were measured. Also, hepatic protein and gene expression levels of lipid metabolism-related factors were analyzed. Results Body weight was decreased in P3% group. In P1% (group fed high-fat diet and 1% Menthae Herba hydrosol) and P3% (group fed high-fat diet and 3% Menthae Herba hydrosol) group, weight of white adipose tissue, serum levels of triglyceride and blood urea nitrogen were decreased, and weight of muscle was increased. Also, liver, kidney and epididymal adipocyte size were reduced in P1% and P3% group. Adenosine monophosphate-activated protein kinase was increased and sterol regulatory element binding protein-1c (SREBP-1c) was decreased in P3% group. mPeroxisome proliferator-activator receptor-γ, mMonocyte chemotactic protein-1 were decreased in P1% and P3% group. In P3% group, mSREBP-1c was decreased and mCarnitine palmitoyl transferase-1 was increased. And mUncoupling protein 1 in brown adipose tissue was increased. Conclusions These results suggest that Menthae Herba hydrosol has a worthy effect on anti-obesity.

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표/그림 (15)

표 Table I. Diet Composition for Animal Experiment
그림 Fig. 1. Gas chromatography-mass spectrometry chromatogram of Menthae Herba hydrosol. The number on the horizontal axis means the retention time of the peak.
표 Table II. Sequences of Primers for Real-Time Polymerase Chain Reaction
그림 Fig. 2. Effect of Menthae Herba hydrosol on body weight change in high-fat diet fed mice. Data are presented as mean±standard deviation (n=7). NDW: group fed normal diet and tap water, HFDW: group fed high-fat diet and tap water, P1%: group fed high-fat diet and 1% Menthae Herba hydrosol, P3%: group fed high-fat diet and 3% Menthae Herba hydrosol. ^abcMeans not sharing a common letter are significantly different among the groups at p<0.05.
그림 Fig. 3. Effect of Menthae Herba hydrosol on water intake in high-fat diet fed mice. Data are presented as mean±standard deviation (n=7). NDW: group fed normal diet and tap water, HFDW: group fed high-fat diet and tap water, P1%: group fed high-fat diet and 1% Menthae Herba hydrosol, P3%: group fed high-fat diet and 3% Menthae Herba hydrosol. ^abMeans not sharing a common letter are significantly different among the groups at p<0.05.
표 Table III. Chemical Profiles of Menthae Herba Hydrosol by Gas Chromatography-Mass Spectrometry
표 Table IV. Effect of Menthae Herba Hydrosol on Body Weight, Food Intake, Caloric Intake and FER in High-Fat Diet Fed Mice
표 Table V. Effect of Menthae Herba Hydrosol on WAT Weights in High-Fat Diet Fed Mice
표 Table VI. Effect of Menthae Herba Hydrosol on Tissue Weights in High-Fat Diet Fed Mice
그림 Fig. 4. Effect of Menthae Herba hydrosol on histomorphology of liver, kidney glomerulus and epididymal WAT in high-fat diet fed mice (original magnification ×200). Arrows indicate change of adipocyte size and distribution. NDW: group fed normal diet and tap water, HFDW: group fed high-fat diet and tap water, P1%: group fed high-fat diet and 1% Menthae Herba hydrosol, P3%: group fed high-fat diet and 3% Menthae Herba hydrosol, WAT: white adipose tissue.
표 Table VII. Effect of Menthae Herba Hydrosol on Hematobiochemical Indices in High-Fat Diet Fed Mice
표 Table VIII. Effect of Menthae Herba Hydrosol on Serum Lipid Profiles in High-Fat Diet Fed Mice
그림 Fig. 5. Effect of Menthae Herba hydrosol on lipid-related protein expressions of liver in high-fat diet fed mice. Data are presented as mean±standard deviation (n=7). (A) Phosphoadenosine monophosphate-activated protein kinase (p-AMPK). (B) Phospho-acetyl-CoA carboxylase (p-ACC). (C) Peroxisome proliferator-activator receptor-γ (PPAR-γ). (D) Sterol regulatory element binding protein-1c (SREBP-1c). (E) Carnitine palmitoyl transferase-1 (CPT-1). (F) HMG-CoA reductase. NDW: group fed normal diet and tap water, HFDW: group fed high-fat diet and tap water, P1%: group fed high-fat diet and 1% Menthae Herba hydrosol, P3%: group fed high-fat diet and 3% Menthae Herba hydrosol. ^abcMeans not sharing a common letter are significantly different among the groups at p<0.05.
그림 Fig. 6. Effect of Menthae Herba hydrosol on lipid metabolismrelated gene expressions of liver in high-fat diet fed mice. Data are presented as mean±standard deviation (n=7). (A) mSterol regulatory element binding protein-1c (mSREBP-1c). (B) mCCAAT enhancer-binding protein-α (mC/EBP-α). (C) mMonocyte chemotactic protein-1 (mMCP-1). (D) mPeroxisome proliferator-activator receptor-γ (mPPAR-γ). (E) mCarnitine palmitoyl transferase-1 (mCPT-1). (F) mHMG-CoA reductase. NDW: group fed normal diet and tap water, HFDW: group fed high-fat diet and tap water, P1%: group fed high-fat diet and 1% Menthae Herba hydrosol, P3%: group fed high-fat diet and 3% Menthae Herba hydrosol. ^abcMeans not sharing a common letter are significantly different among the groups at p<0.05.
그림 Fig. 7. Effect of Menthae Herba hydrosol on gene expressions of brown adipose tissue in high-fat diet fed mice. Data are presented as mean±standard deviation (n=7). mUCP: mUncoupling protein 1, mPPAR-γ: mPeroxisome proliferator-activator receptor- γ, NDW: group fed normal diet and tap water, HFDW: group fed high-fat diet and tap water, P1%: group fed high-fat diet and 1% Menthae Herba hydrosol, P3%: group fed high-fat diet and 3% Menthae Herba hydrosol. ^abcMeans not sharing a common letter are significantly different among the groups at p<0.05.

참고문헌 (37)

Peng Y, Yu S, Li H, Xiang H, Peng J, Jiang S. MicroRNAs:？emerging roles in adipogenesis and obesity. Cellular？Signalling. 2014;26(9):1888-96.

상세보기
Na SY, Myung SJ. Obesity and colorectal cancer. Korean？Journal of Gastroenterology. 2012;59(1):16-26.

상세보기
Jensen MD, Ryan DH, Apovian CM, Ard JD, Comuzzie？AG, Donato KA. 2013 AHA/ACC/TOS guideline for？the management of overweight and obesity in adults:？a report of the American College of Cardiology/American？Heart Association Task Force on Practice Guidelines and？The Obesity Society. Circulation. 2014;129(25):102-38.
Lee SY. Medical management of obesity. Korean Journal？of Helicobacter and Upper Gastrointestinal Research.？2017;17(2):66-71.

상세보기
James WP, Caterson ID, Coutinho W, Finer N, Van Gaal？LF, Maggioni AP. Effect of sibutramine on cardiovascular？outcomes in overweight and obese subjects. New England？Journal of Medicine. 2013;363(10):905-17.
Chang SH, Carolyn S, Song JH, Esteban V, Christopher？J, Graham C. The effectiveness and risks of bariatric？surgery an updated systematic review and meta-analysis, 2003-2012. JAMA Surgery. 2014;139(3):275-87.
Sogo E, Zhou S, Haeiwa H, Takeda R, Okazaki K, Sekita？M, Yamamoto T, Yamano M, Sakamoto K. Amber extract reduces lipid content in mature 3T3-L1 adipocytes？by activating the lipolysis pathway. Molecules. 2021;26(15):4630.

상세보기
The Society of Korean Medicine Rehabilitation. Korean？rehabilitation medicine 4th ed. Koonja. 2015:303-5.
Kim SJ, Seo YH, Lee HS, Chang HK, Cho JH, Kim？KW, Song MY. Research trends of herbal medicines for？obesity: mainly since 2015 to 2019. J Korean Med Rehabil.？2020;30(4):89-103.
Korean Medical College Society Textbook Compilation？Committee. The herbology. Younglimsa. 2008:177-9.
Cho HM, Sowndhararajan K, Jung JW, Jhoo JW, Kim？SM. Fragrance chemicals in the essential oil of Mentha？arvensis reduce levels of mental stress. Life Sciences.？2013;23(7):933-40.
Lim HS, Kim JH, Ha HK, Seo CS, Shin HK. Compar ative？study of the anti-inflammatory effects of Menthae Herba？from Korea and China. Korean Journal of Pharmacognosy.？2012;43(3):231-8.
Hong SW, Kim HA, Lee JM. Nutritional compositions？of Lysimachia christinae Hance and Mentha canadensis？and effect of Lysimachia christinae Hance on antioxidant？properties. Korean Journal of Community Living Science.？2019;30(3):351-61.
Lee JH, Kim HJ, Jang GY, Seo KH, Kim MR, Choi？YH, Jung JW. Mentha arvensis attenuates congnitive？and memory impairment in scopolamine-treated mice.？Korean Journal of Pharmacognosy. 2020;51(1):70-7.
Badal RM, Badal D, Badal P, Khare A, Shrivastava J.？Kumar V. Pharmacological action of Mentha piperita？on lipid profile in fructose-fed rats. Iranian Journal of？Pharmaceutical Research. 2011;10(4):843-8.

상세보기
Vizin RCL, Motzko-Soares ACP, Armentano GM, Ishikawa？DT, Cruz-Neto AP, Carrettiero DC, Almeida MC. Short-term menthol treatment promotes persistent thermogenesis without induction of compensatory food consumption in wistar rats: implications for obesity control.？Journal of Applied Physiology. 2018;124(3):672-83.

상세보기
Jiang CY, Zhai MZ, Yan D, Li C, Zhang YH, Xiao？L, Xiong DJ, Deng Q, Sun WP. Dietary menthol-induced TRPM8 activation enhances WAT "browning"？and ameliorates diet-induced obesity. Oncotarget. 2017;8(43):75114-26.

상세보기
Wojcik-Stopczynska B, Jakowienko P, Wysocka G. The？estimation of antifungal activity of essential oil and hydrosol obtained from wrinkled-leaf mint (Mentha crispa？L.). Herba Polonica. 2012;58(1):5-15.
Kim MR, Choi BH, Kim HJ, Jo EB, Choi HA, Hong？JI. Effects of zinc protoporphyrin on color responses？of the Bradford and BCA protein assays. Journal of？Natural Science. 2012;24:57-64.
Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nature Protocols.？2008;3(6):1101-8.

상세보기
Kim CS. Recent advances for anti-obesity agents. Journal？of Korean Diabetes. 2015;16(4):260-8.
Houg HJ, Yang HY, Kang WY, Kim DG, Lee SY, Choi？YH, Kim JH, Han CH, Lee YJ. Dangyuia (Citrus grandis Osbeck) peel improves lipid profiles and alleviates？hypertension in rats fed a high-fat diet. Laboratory？Animal Research. 2010;26(4):361-7.
de Sousa Guedes JP, da Costa Medeiros JA, de Souza？e Silva RS, de Sousa JN, da Conceicao ML, de Souza？EL. The efficacy of Mentha arvensis L. and M. piperita？L. essential oils in reducing pathogenic bacteria and？maintaining quality characteristics in cashew, guava,？mango, and pineapple juices. International Journal of？Food Microbiology. 2016;238:183-92.

상세보기
Benabdallah A, Boumendjel M, Aissi O, Rahmoune C,？Boussaid M, Messaoud C. Chemical composition, antioxidant activity and acetylcholinesterase inhibitory of？wild Mentha species from northeastern Algeria. South？African Journal of Botany. 2018;116:131-9.
Baik JA, Baik JP, Cho Y, Chiang MH. Effect of floral？water extracted from Elsholtzia ciliata, Acorus gramineus and Zanthoxylum schinifolium on quality and prolongation of vase life in cut rose. Journal of Natural？Science. 2013;25:185-92.
Makkar MK, Shar ma S, Kaur H. Evaluation of Mentha？arvensis essential oil and its major constituents for？fungitoxicity. Journal of Food Science and Technology.？2018;55(9):3840-4.

상세보기
Mamta B, Pande C, Tewari G. Effect of copper amendments on the quality of essential oils extracted from？the aerial parts of Mentha arvensis L. Journal of Essential？Oil-bearing Plants. 2021;24(2):193-200.

상세보기
Saely CH, Geiger K, Drexel H. Brown versus white？adipose tissue: a mini-review. Gerontology. 2012;58(1):15-23.

상세보기
Jung DH, Kim JH. Relationships among muscle mass？and obesity, metabolic syndrome, physical activity, and？nutrient intake in elderly women: based on the 4th-5th？(2008-2011) Korean national health and nutrition examination survey (KNHANES). Korean Journal of Community？Living Science. 2018;29(4):469-83.
Kato S, Nazneen A, Nakashima Y, Rassaque MS, Nishino？T, Furusu A. Pathological influence of obesity on renal？structural changes in chronic kidney disease. Clinical and？Experimental Nephrology. 2009;13(4):332-40.

상세보기
Choi MRN, Ha SM, Kim DY. Effects of elastic band？exercise on body composition, blood lipids and AMPK？in the elderly women. Journal of Korean Applied Science？and Technology. 2019;36(3):995-1007.
White UA. Stephens JM. Transcriptional factors that？promote formation of white adipose tissue. Molecullar？and Cellular Endocrinolgy. 2010;318(1-2):10-4.
Yang X. Han M, Liu S, Yuan X, Liu X, Feng S, Zhou？L, Li Y, Lu H, Cheng J, Lin S. HCBP6 upregulates？human SREBP1c expression by binding to C/EBPβ-binding site in the SREBP1c promoter. BMB Reports.？2018;51(1):33-8.
Park YM, Lim JH, Seo EW. Anti-obesity effect of？by-product from soybean on mouse fed a high fat diet.？Korean Journal of Plant Resources. 2015;28(2):168-77.
Wang HJ, Park JY, Kwon OB, Choe EY, Kim CH, Hur？KY, Lee MS, Yun MJ, Cha BS, Kim YB, Lee HK, Kang？ES. Chronic HMGCR/HMG-CoA reductase inhibitor？treatment contributes to dysglycemia by upregulating？hepatic gluconeogenesis through gutophagy induction.？Autography. 2015;11(11):2089-101.
Kim SH, Par k BB, Hong SE, Ryu SR, Lee JH, Kim？SH, Lee PJ, Cho EK, Moon C. Effects of 2-methoxy1,4-naphthoquinone (MQ) on MCP-1 induced THP-1？migration. Korean Journal of Clinical Laboratory Science.？2019;51(2):245-51.
You YW, Lee CW, Jung JH, Kim YC, Lee DE, Park？SH, Kim TS. Factors influencing the activation of brown？adipose tissue in 18F-FDG PET/CT in national cancer？center. Korean Journal of Nuclear Medicine Technology.？2021;25(1):21-8.

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