[논문]Association of single-nucleotide polymorphisms in dual specificity phosphatase 8 and insulin-like growth factor 2 genes with inosine-5'-monophosphate, inosine, and hypoxanthine contents in chickens

Jean Pierre Munyaneza; Minjun Kim; Eunjin Cho; Aera Jang; Hyo Jun Choo; Jun Heon Lee

doi:10.5713/ab.23.0080

Association of single-nucleotide polymorphisms in dual specificity phosphatase 8 and insulin-like growth factor 2 genes with inosine-5'-monophosphate, inosine, and hypoxanthine contents in chickens 원문보기

Animal bioscience, v.36 no.9, 2023년, pp.1357 - 1366

Jean Pierre Munyaneza (Division of Animal and Dairy Science, Chungnam National University) , Minjun Kim (Division of Animal and Dairy Science, Chungnam National University) , Eunjin Cho (Department of Bio-AI Convergence, Chungnam National University) , Aera Jang (Department of Applied Animal Science, College of Animal Life Science, Kangwon National University) , Hyo Jun Choo (Poultry Research Institute, National Institute of Animal Science) , Jun Heon Lee (Division of Animal and Dairy Science, Chungnam National University)

Abstract ▼ AI-Helper

Objective: This study aimed to identify the single-nucleotide polymorphisms (SNPs) in the dual-specificity phosphatase 8 (DUSP8) and insulin-like growth factor 2 (IGF2) genes and to explore their effects on inosine-5'-monophosphate (IMP), inosine, and hypoxanthine contents in Korean native chicken -red-brown line (KNC-R Line). Methods: A total sample of 284 (males, n = 127; females n = 157) and 230 (males, n = 106; females, n = 124) aged of 10 weeks old KNC-R line was used for genotyping of DUSP8 and IGF2 genes, respectively. One SNP (rs313443014 C>T) in DUSP8 gene and two SNPs (rs315806609A/G and rs313810945T/C) in IGF2 gene were used for genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and KASP methods, respectively. The Two-way analysis of variance of the R program was used to associate DUSP8 and IGF2 genotypes with nucleotide contents in KNC-R chickens. Results: The DUSP8 (rs313443014 C>T) was polymorphic in KNC-R line and showed three genotypes: CC, CT, and TT. The IGF2 gene (rs315806609A/G and rs313810945T/C) was also polymorphic and had three genotypes per SNP, including GG, AG, and AA for the SNP rs315806609A/G and genotypes: CC, CT, and TT for the SNP rs313810945T/C. Association resulted into a strong significant association (p<0.01) with IMP, inosine, and hypoxanthine. Moreover, the significant effect of sex (p<0.05) on nucleotide content was also observed. Conclusion: The SNPs in the DUSP8 and IGF2 genes might be used as genetic markers in the selection and production of chickens with highly flavored meat.

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표/그림 (5)

표 Table 1. Primer information, SNP, PCR product (bp), annealing temperature, and genotyping method for DUSP8 and IGF2 genes
그림 Figure 1. RFLP genotyping results of DUSP8 gene (SNP rs313443014C>T) visualized on 3% agarose gel electrophoresis from samples 1 to 8 (A); KASP genotyping results for SNP in intron 1(rs315806609A/G) (B); and synonymous SNP (rs313810945T/C) (C) of IGF2 gene in Korean native chicken-red-brown line (KNC-R line). RFLP, restriction fragment length polymorphism; DUSP8, dual-specificity phosphatase 8; KASP, Kompetitive allele-specific polymerase chain reaction; SNP, single-nucleotide polymorphism; NEG, negative control; M, DNA marker with 100 bp.
표 Table 2. Genotype frequency, allele frequency, and chi-square test results in the DUSP8 and IGF2 genes of the KNC-R line
표 Table 3. Association of DUSP8 and IGF2 genotypes with IMP, inosine, and hypoxanthine contents in the KNC-R line
표 Table 4. Effects of sex on IMP, inosine, and hypoxanthine contents in KNC-R line

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표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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