[논문]보음전의 항산화, 항염증 효능에 관한 실험연구

김수경; 조성희; 양승정

doi:10.15204/jkobgy.2023.36.3.001

보음전의 항산화, 항염증 효능에 관한 실험연구
The Experimental Study on Antioxidant, Anti-inflammatory Effects of the Boeum-jeon (BEJ) 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.36 no.3, 2023년, pp.1 - 24

김수경 (봄봄한의원) , 조성희 (동신대학교 한의과대학 한방부인과교실) , 양승정 (동신대학교 한의과대학 한방부인과교실)

Abstract ▼ AI-Helper

Objectives: Boeum-jeon (BEJ) is a herbal formula composed 8 Korean medicinal herbs and is traditionally used to treat inflammatory diseases. In this study, the authors tried to confirm the antioxidant efficacy of BEJ and its anti-inflammatory effect in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Methods: In this experiment, results of BEJ on the following two were measured as follows: (1) Antioxidant effects was measured by DPPH Radical scavenging Activity, ABTS Radical scavenging Activity. (2) Anti-Inflammatory effects were evaluated by the production amount of ROS, NO, Cytokine (PGE2, IL-1β, IL-6, TNF-α), COX-2, iNOS, TNF-α, IL-1β, IL-6, HO-1, NQO1 (the previous seven are "mRNA Expression"), COX-2, iNOS, TNF-α, IL-1β, IL-6, HO-1, NQO1, NRF2 (the previous eight are "Protein Expression") ERK, JNK, p38 (the previous three are "Protein Phosphorylation") in LPS-stimulated RAW 264.7 cells. Results: The experimental measurement results are as follows: (1) DPPH Radical scavenging Activity, ABTS Radical scavenging Activity increased in a concentration -dependent manner in the BEJ-treated group. (2) As a result of measuring anti-inflammatory efficacy, the production of ROS, NO, and Cytokine (PGE2, TNF-α, IL-1β, IL-6) in the BEJ-administered group was significantly reduced compared to the control group. (3) Among mRNA Expression levels, COX-2, iNOS, IL-1β, IL-6 and TNF-α was significantly decreased in a concentration-dependent manner than in the control group, and HO-1 and NQO1 were significantly increased in a concentration -dependent manner than in the control group. (4) Among the Protein Expression levels, COX-2, iNOS, IL-1β, IL-6 and TNF-α was significantly decreased in a concentration -dependent manner compared to the control group, and HO-1, NQO1 and NRF2 was significantly increased in a concentration-dependent manner compared to the control group. (5) As a result of Protein Phosphorylation, ERK, p38 and JNK was significantly decreased compared to the control group in a concentration-dependent manner. Conclusions: Boeum-jeon has been experimentally confirmed to have antioxidant and anti-inflammatory effects, and if the evidence for efficacy is reinforced through further studies such as in vivo studies and clinical trials in the future, it can be effectively used to treat various inflammatory diseases such as bladder inflammation and chronic pelvic inflammation.

주제어

표/그림 (26)

표 Table 1. The Prescription of BEJ
표 Table 2. Real-Time PCR Primer Sequences
그림 Fig. 1. Cell viability of BEJ in RAW 264.7 cells.
그림 Fig. 2. DPPH radical scavenging activity of BEJ.
그림 Fig. 3. ABTS radical scavenging activity of BEJ.
그림 Fig. 4. Effect of BEJ on reactive oxygen species level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 5. Effect of BEJ on nitric oxide level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 6. Effect of BEJ on prostaglandin E2 level in RAW 264.7 cells. * : p<0.05, ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 7. Effect of BEJ on interleukin 1 beta level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 8. Effect of BEJ on interleukin 6 level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 9. Effect of BEJ on tumor necrosis factor alpha level in RAW 264.7 cells. * : p<0.05, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 10. Effect of BEJ on iNOS mRNA expression level in RAW 264.7 cells. * : p<0.05, ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 11. Effect of BEJ on COX-2 mRNA expression level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 12. Effect of BEJ on IL-1β mRNA expression level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 13. Effect of BEJ on IL-6 mRNA expression level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 14. Effect of BEJ on TNF-α mRNA expression level in RAW 264.7 cells. *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 15. Effect of BEJ on HO-1 mRNA expression level in RAW 264.7 cells. ** : p<0.01 compared to control
그림 Fig. 16. Effect of BEJ on NQO1 mRNA expression level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control
그림 Fig. 17. Effect of BEJ on iNOS protein expression level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 18. Effect of BEJ on COX-2 protein expression level in RAW 264.7 cells. *** : p<0.001 compared to control, ++ : p<0.01 compared to normal
그림 Fig. 19. Effect of BEJ on IL-1β protein expression level in RAW 264.7 cells. *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 20. Effect of BEJ on IL-6 protein expression level in RAW 264.7 cells. ** : p<0.01, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 21. Effect of BEJ on TNF-α protein expression level in RAW 264.7 cells. * : p<0.05, *** : p<0.001 compared to control, +++ : p<0.001 compared to normal
그림 Fig. 22. Effect of BEJ on HO-1 protein expression level in RAW 264.7 cells. *** : p<0.001 compared to control
그림 Fig. 23. Effect of BEJ on NQO1 protein expression level in RAW 264.7 cells. *** : p<0.001 compared to control
그림 Fig. 24. Effect of BEJ on NRF2 protein expression level in RAW 264.7 cells. *** : p<0.001 compared to control

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표제어: PCR

동의어: Packet Collision Rate

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