[논문]표고 톱밥배지 영양원, 접종 및 배양조건에 따른 균사배양 및 자실체 특성 비교

정연석; 김민준; 장영선; 가강현

doi:10.4489/kjm.20230008

표고 톱밥배지 영양원, 접종 및 배양조건에 따른 균사배양 및 자실체 특성 비교
Comparison of Mycelium Cultivation and Fruiting Body Characteristics of Lentinula edodes According to the Sawdust Media Nutrients and Inoculation and Cultivation Conditions 원문보기

한국균학회지 = The Korean journal of mycology, v.51 no.2, 2023년, pp.69 - 80

정연석 (국립산림과학원 산림생명자원연구부 산림미생물연구과) , 김민준 (국립산림과학원 산림생명자원연구부 산림미생물연구과) , 장영선 (국립산림과학원 산림생명자원연구부 산림미생물연구과) , 가강현 (국립산림과학원 산림생명자원연구부 산림미생물연구과)

초록
AI-Helper

표고버섯의 배양조건은 버섯의 생산량과 품질에 많은 영향을 준다. 본 연구에서는 표고버섯의 배양조건을 변화시켜 생산성 및 품질을 개량하기 위한 연구를 수행하였다. 액체와 고체 두 가지 형태의 종균을 사용하였고, 톱밥배지의 영양원으로 옥수수가루를 사용하였다. 명배양 과정에서는 광조건을 청색광으로 변화시켜 연구를 수행하였다. 실험품종으로는 산백향을 이용하였다. 옥수수가루를 이용한 결과 배양 21일까지의 균사생장률이 최대 2.7배 빠른 것으로 나타났으며, 배지의 중량 감소율이 더 높은 것으로 나타났다. 액체종균을 사용한 실험군에서는 톱밥종균을 이용하였을 때 보다 자실체의 생산량이 1.2배 증가하는 것을 확인할 수 있었으며, 청색광을 이용한 실험군에서는 1.1배의 생산량이 증가하였다. 자실체의 무게와 갓 크기는 톱밥종균을 사용한 경우 더 높게 나타났으며, 버섯의 무게 및 대두께는 청색광을 이용한 조건에서 더 우월한 것으로 나타났다. 전자혀를 통한 맛 분석 결과 옥수수가루를 사용하였을 때 짠맛이 증가하였고, 청색광을 사용하면 시큼한 맛이 증가하는 것을 확인할 수 있었다.

Abstract ▼ AI-Helper

The cultivation conditions of shiitake mushrooms (Lentinula edodes) influence the production and quality of fruiting bodies. We conducted this study to improve the productivity and quality of shiitake mushrooms by modifying the cultivation conditions. Two types of spawns (sawdust and liquid spawn) were used, and corn flour was used as a nutritional source for the sawdust medium. A blue light-emitting diode (LED; 300 lux) was also used instead of a white LED during the incubation period. Sanbaekhyang was used as the experimental variety. When using corn flour, the mycelial growth rate increased 1.1 to 2.7 times the growth rate of the control up to 21 days of incubation, and the weight loss rate of the media was also higher. Mushroom productivity increased 1.2 times when the liquid spawn was used compared to when the sawdust spawn was used, and the blue LED also increased fruiting body production by 1.1 times compared to the white LED. Mushroom productivity increased when the liquid spawn was used, and the blue LED also increased fruiting body production. Fruiting body weight and the size of the cap were greater when sawdust spawn was used. The fruiting body weight and the stipe diameter were greater when the blue LED was used. Taste analysis showed that the saltiness increased when corn flour was used, and the sourness increased when the blue LED was used.

주제어

표/그림 (6)

표 Table 1. Substrate formula and experimental condition used in Lentinula edodes cultivation.
그림 Fig. 1. Comparison of weight loss rate (%) of sawdust media after 100 days under different cultivation conditions. a-d: The value indicates a significant diference (Tukey test p<0.05).
그림 Fig. 2. Initial mycelial growth on the surface of sawdust medium. (A) Sawdust inoculum, (B) Liquid medium. a-c: The value indicates a significant difference (Tukey test p<0.05).
표 Table 2. Comparison of morphological characteristics of fruiting bodies under different cultivation conditions.
그림 Fig. 3. Comparison of fruiting body production of 'Sanbaekhyang' on sawdust media under different cultivation conditions. a, b: The value indicates a significant diference (Tukey test p<0.05).
그림 Fig. 4. Intensity comparison score radar chart of rolled-dumplings with various levels of hot-air dried (50℃) 'Sanbaekhyang' using taste sensor of e-tongue. (A) Sawdust inoculum, (B) Liquid inoculum. PKS, auxiliary sensor; CPS, standard sensor.

참고문헌 (25)

Korea Forest Service. 2020 Production of forest products. Daejeon: Korea Forest Service; 2021.
Bak WC, Lee EY, Yoon KH, Lee WK, Yi CK, Hong KS. Growth and rotting characteristics？of Lentinula edodes isolated in Korea and their adaptability to bag-culture using oak sawdust.？Jour Korean For Soc 1994;83:12-9.
Statistics Korea. 2020 Agriculture, forestry & fishery census report. Forestry (Vol. 2, No. 2).？Daejeon: Statistics Korea; 2021.
National Institute of Forest Science. Forest science bulletin 21-47. Golden seed. Cultivation of？Lentinula edodes cultivar Sanbaekhyang using columnar shape sawdust bag. Suwon: National？Institute of Forest Science; 2021.
National Institute of Forest Science. Forest science bulletin 21-48. Golden seed. Cultivation？of Lentinula edodes cultivar Sanbaekhyang using cylindrical shape sawdust bag. Suwon:？National Institute of Forest Science; 2021.
National Institute of Forest Science. Forest science bulletin 21-49. Golden seed. Cultivation？of Lentinula edodes cultivar Sanbaekhyang using rectangular shape sawdust bag. Suwon:？National Institute of Forest Science; 2021.
National Institute of Forest Science. 2021 Research project report. Forestry income increase？and utilizing industrialization by using forest bioresources. Seoul: National Institute of Forest？Science; 2022.
Jang Y, Jeong YS, Ryoo R, Ka KH. Comparison of cultivation, mushroom yield, and fruiting？body characteristics of Lentinula edodes strains according to the inoculation method. Kor J？Mycol 2021;49:525-30.
Choi DS, Jung KJ. The optimum media size and composition for sawdust culture of Lentinula？edodes (Strain: JNM3022). J Mushrooms 2008;6:131-7.
Jang MJ, Lee YH, Lee HB, Liu JJ, Ju YC. Comparison in cultural characteristics on different？nutritions in bag cultivation of Lentinula edodes. J Mushrooms 2011;9:105-9.
Kim YH, Jhune CS, Park SC, You CH, Sung JM, Kong WS. The effect of environmental？condition to the mycelial browning of Lentinula edodes (Berkeley) Sing. During sawdust bag？cultivation. J Mushrooms 2009;7:115-21.
Korea Forest Seed&Variety Center. Guidelines for the conduct of DUS tests of Lentinula？edodes. Chungju: Korea Forest Seed&Variety Center; 2014. p. 22-39.
Zeng Z, Wang J, Wen X, Wang Y, Li X, Liu D, Geng F. Metabolomic analysis provides insights？into the mechanism of color and taste changes in Dictyophora indusiata fruiting bodies under？different drying processes. Food Res Int 2022;162:112090.

상세보기
Jeong YS, Jang YS, Rhim R, Ka KH. Utilization of various substrates for the cultivation of oak？mushrooms (Lentinula edodes). Kor J Mycol 2019;47:373-83.
Zhang YY, Fang YM, YU K, Li XX, Xia T. Molecular characterization and genetic structure of？Quercus acutissima germplasm in China using microsatellites. Mol Biol Rep 2013;40:4083-90.

상세보기
Yu H, Zhang D, Zhang L, Li Q, Song C, Shang X, Bao D, Tan Q, Chen H, Lv B. Corncob as a？substrate for the cultivation of Lentinula edodes. Waste Biomass Valorization 2022;13:929-39.
Zou Y, Du F, Hu Q, Yuan X, Dai D, Zhu M. Integration of Pleurotus tuoliensis cultivation and？biogas production for utilization of lignocellulosic biomass as well as its benefic evaluation.？Bioresour Technol 2020:124042.
Bach F, Helm CV, De Lima EA, Bellettini MB, Haminiuk CWI. Influence of cultivation？methods on the chemical and nutritional characteristics of Lentinula edodes. Emir J Food？Agric 2018;30:1006-13.
Hoa HT, Wang CL, Wang CH. The effects of different substrates on the growth, yield, and？nutritional composition of two oyster mushrooms (Pleurotus ostreatus and Pleurotus？cystidiosus). Mycobiology 2015;43:423-34.

원문보기 상세보기
Sano H, Narikiyo T, Yamazaki T, Shishido K. Sequence analysis and expression of a blue-light？photoreceptor gene, Le.phrA from the basidiomycetous mushroom Lentinula edodes. Biosci Biotechnol Biochem 2007;71:2206-13.

상세보기
Sakamoto Y, Tamai Y, Yajima T. Influence of light on the morphological changes that？take place during the development of the Flammulina velutipes fruit body. Mycoscience？2004;45:333-9.

상세보기
Kim JY, Kim DY, Park YJ, Jang MJ. Transcriptome analysis of the edible mushroom Lentinula？edodes in response to blue light. Plus One 2020;15:1-18.
Tang LH, Jian HH, Song CY, Bao DP, Shang XD, Wu DQ, Tan Q, Zhang XH. Transcriptome？analysis of candidate gene and signaling pathways associated with light-induced brown film？formation in Lentinula edodes. App Microbiol Biotechnol 2013;97:4977-89.
Go HY, Lee SH, Kim HY. The effect of hot-air dried Lentinula edodes on the quality and？oranoleptic properties of rolled-dumplings. Food Sci Anim Resour 2022;42:593-608.

원문보기 상세보기
Kong Y, Zhang LL, Zhao J, Zhang YY, Sung BG, Chen HT. Isolation and identification of the？umami peptides from shiitake mushroom by consecutive chromatography and LC-Q-TOF-MS.？Food Res Int 2019;121:463-70.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증